A Novobiocin Derivative, XN4, Inhibits the Proliferation of Chronic Myeloid Leukemia Cells by Inducing Oxidative DNA Damage

• Published in: PloS one Vol. 10; no. 4; p. e0123314
• Main Authors: Wu, Lixian, Chen, Xianling, Huang, Lisen, Tian, Jue, Ke, Fang, Xu, Jianhua, Chen, Yuanzhong, Zheng, Ming
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30.04.2015; Public Library of Science (PLoS)
• Subjects: Acetylcysteine; Antigens, CD34 - genetics; Antigens, CD34 - metabolism; Apoptosis; Apoptosis - drug effects; Apoptosis - genetics; Bone marrow; Bromine compounds; Cancer genetics; Caspase; Caspase-3; Cell cycle; Cell Cycle - drug effects; Cell Cycle - genetics; Cell death; Cell Line, Tumor; Cell proliferation; Cell Proliferation - drug effects; Cell Proliferation - genetics; Cell Survival - drug effects; Cell Survival - genetics; Chronic myeloid leukemia; Cytometry; Damage; Deoxyribonucleic acid; DNA; DNA damage; DNA Damage - drug effects; DNA Damage - genetics; Flow Cytometry; Humans; Immunoblotting; Intracellular signalling; K562 Cells; Leukemia; Membrane Proteins - genetics; Membrane Proteins - metabolism; mRNA; Myeloid leukemia; NAD(P)H oxidase; NADPH Oxidase 4; NADPH Oxidase 5; NADPH Oxidases - genetics; NADPH Oxidases - metabolism; Novobiocin; Novobiocin - analogs & derivatives; Novobiocin - pharmacology; NOX4 protein; Oxidases; Oxygen; Poly(ADP-ribose) polymerase; Reactive oxygen species; Reactive Oxygen Species - metabolism; Real-Time Polymerase Chain Reaction; RNA; Stem cells

XN4 might induce DNA damage and apoptotic cell death through reactive oxygen species (ROS). The inhibition of proliferation of K562 and K562/G01 cells was measured by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide). The mRNA levels of NADPH oxidase 1-5 (Nox1-5) genes were evaluate...