Inhibition of Tankyrases Induces Axin Stabilization and Blocks Wnt Signalling in Breast Cancer Cells

• Published in: PloS one Vol. 7; no. 11; p. e48670
• Main Authors: Bao, Renyue, Christova, Tania, Song, Siyuan, Angers, Stephane, Yan, Xiaojun, Attisano, Liliana
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07.11.2012; Public Library of Science (PLoS)
• Subjects: Adenomatous polyposis coli; Adenosine diphosphate; ADP-ribosylation; Animal sciences; Animals; Attenuation; Axin Protein - metabolism; Biochemistry; Biology; Breast cancer; Breast Neoplasms - enzymology; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cancer; Cancer cells; Cell growth; Cell Line, Tumor; Cell migration; Cell Movement - drug effects; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - enzymology; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Combinatorial analysis; Culture Media, Conditioned; Deprivation; Enzymes; Genetic aspects; HEK293 Cells; Heterocyclic Compounds, 3-Ring - pharmacology; Humans; Inhibition; Kinases; MCF-7 Cells; Medicine; Mice; Mutation; Poly(ADP-ribose) polymerase; Proteins; Ribosylation; RNA Interference; Signaling; siRNA; Stabilization; Studies; Tankyrases - antagonists & inhibitors; Transcription; Wnt protein; Wnt Signaling Pathway - drug effects; β-Catenin

Constitutive Wnt signalling is characterized by excessive levels of β-catenin protein and is a frequent occurrence in cancer. APC and Axin are key components of the β-catenin destruction complex that acts to promote β-catenin degradation. The levels of Axin are in turn controlled by tankyrases, memb...