Self-digitization chip for single-cell genotyping of cancer-related mutations

• Published in: PloS one Vol. 13; no. 5; p. e0196801
• Main Authors: Thompson, Alison M., Smith, Jordan L., Monroe, Luke D., Kreutz, Jason E., Schneider, Thomas, Fujimoto, Bryant S., Chiu, Daniel T., Radich, Jerald P., Paguirigan, Amy L.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 02.05.2018; Public Library of Science (PLoS)
• Subjects: Acute myelocytic leukemia; Acute myeloid leukemia; Alleles; Biology and Life Sciences; Biomedical microelectromechanical systems; Cancer; Digitization; DNA sequencing; Genetic aspects; Genetic variability; Genetic variation; Genotyping; Genotyping Techniques; Health aspects; Heterogeneity; Leukemia; Methods; Microfluidics; Mutation; Mutation - genetics; Myeloid leukemia; Neoplasms - genetics; Oligonucleotide Array Sequence Analysis; Physical Sciences; Quality assessment; Quality control; Research and Analysis Methods; Tumors; Variability; Zygosity

Cancer is a heterogeneous disease, and patient-level genetic assessments can guide therapy choice and impact prognosis. However, little is known about the impact of genetic variability within a tumor, intratumoral heterogeneity (ITH), on disease progression or outcome. Current approaches using bulk...