Deciphering the prokaryotic community and metabolisms in South African deep-mine biofilms through antibody microarrays and graph theory

• Published in: PloS one Vol. 9; no. 12; p. e114180
• Main Authors: Blanco, Yolanda, Rivas, Luis A, García-Moyano, Antonio, Aguirre, Jacobo, Cruz-Gil, Patricia, Palacín, Arantxa, van Heerden, Esta, Parro, Víctor
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.12.2014; Public Library of Science (PLoS)
• Subjects: Anaerobic microorganisms; Antibodies; Antibodies, Archaeal - metabolism; Antibodies, Bacterial - metabolism; Antibody microarrays; Antigens; Archaea - genetics; Archaea - immunology; Archaea - metabolism; Archaea - physiology; Bacteria - genetics; Bacteria - immunology; Bacteria - metabolism; Bacterial Physiological Phenomena; Biofilms; Biofilms - growth & development; Biology and Life Sciences; Biomarkers; Communities; Computer Graphics; Cross-reactivity; Deoxyribonucleic acid; DNA; DNA microarrays; Earth Sciences; Ecology and Environmental Sciences; Environmental monitoring; Evolution; Fermentation; Fractures; Galleries; Gene sequencing; Genomes; Geobiology; Gold; Gold mines & mining; Graph theory; Immunoassay; Immunoglobulins; Immunology; Intersections; Medical research; Methanogenesis; Methanogenic bacteria; Microbial activity; Microorganisms; Mines; Mining; Monitoring methods; Nitrate reduction; Open systems; Oxidation; Oxidizing agents; Oxygen; Oxygen - metabolism; Phylogeny; Protein Array Analysis; Proteins; RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Seepage; Sequence Analysis, DNA; South Africa; Storage conditions; Studies; Sulfate; Sulfates; Sulfur; South Africa

In the South African deep mines, a variety of biofilms growing in mine corridor walls as water seeps from intersections or from fractures represents excellent proxies for deep-subsurface environments. However, they may be greatly affected by the oxygen inputs through the galleries of mining activiti...