Complexation of C6-Ceramide with Cholesteryl Phosphocholine – A Potent Solvent-Free Ceramide Delivery Formulation for Cells in Culture

• Published in: PloS one Vol. 8; no. 4; p. e61290
• Main Authors: Sukumaran, Pramod, Lönnfors, Max, Långvik, Otto, Pulli, Ilari, Törnquist, Kid, Slotte, J. Peter
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.04.2013; Public Library of Science (PLoS)
• Subjects: Animals; Apoptosis; Apoptosis - drug effects; Bioavailability; Biochemistry; Biological activity; Biological effects; Biology; Calcium (mitochondrial); Calcium - metabolism; Calcium homeostasis; Calcium influx; Cell culture; Cell growth; Cell membranes; Cell proliferation; Cell Proliferation - drug effects; Cells, Cultured; Ceramide; Ceramides; Ceramides - metabolism; Ceramides - pharmacology; Chains; Chemistry; Cholesterol; Comparative analysis; Cytosol - drug effects; Cytosol - metabolism; Diastereomers; Dimethyl sulfoxide; Dimethyl Sulfoxide - pharmacology; Glycerol; Homeostasis; Homeostasis - drug effects; Humans; Hydrophobicity; Kinases; Lipids; Membranes; Mitochondria; Mitochondria - drug effects; Mitochondria - metabolism; Phosphocholine; Phosphorylcholine - chemistry; Phosphorylcholine - metabolism; Plant lipids; Proteins; Rats; Solvents; Solvents - chemistry; Studies; Thyroid; Thyroid gland; Tritium - metabolism; Finland

Ceramides are potent bioactive molecules in cells. However, they are very hydrophobic molecules, and difficult to deliver efficiently to cells. We have made fluid bilayers from a short-chain D-erythro-ceramide (C6-Cer) and cholesteryl phosphocholine (CholPC), and have used this as a formulation to d...