Suppression of Vimentin Phosphorylation by the Avian Reovirus p17 through Inhibition of CDK1 and Plk1 Impacting the G2/M Phase of the Cell Cycle

• Published in: PloS one Vol. 11; no. 9; p. e0162356
• Main Authors: Chiu, Hung-Chuan, Huang, Wei-Ru, Liao, Tsai-Ling, Wu, Hung-Yi, Munir, Muhammad, Shih, Wing-Ling, Liu, Hung-Jen
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07.09.2016; Public Library of Science (PLoS)
• Subjects: Agricultural biotechnology; Animals; Ataxia Telangiectasia Mutated Proteins - metabolism; Binding sites; Biology and Life Sciences; CDC2 Protein Kinase - metabolism; cdc25 Phosphatases - metabolism; Cell cycle; Cell Cycle Proteins - metabolism; Cell Division; Cell lines; Cell Proliferation; Checkpoint Kinase 1 - metabolism; Chick Embryo; CHK1 protein; Chlorocebus aethiops; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; Deactivation; Deoxyribonucleic acid; DNA; DNA damage; Down-Regulation; Foot & mouth disease; G2 Phase; Gene expression; Genomes; Goods and services tax; Immunoprecipitation; In vivo methods and tests; Inactivation; Inhibition; Intermediate filament proteins; Kinases; Models, Biological; Molecular biology; Nuclear Pore Complex Proteins - metabolism; Orthoreovirus, Avian - metabolism; p17 Protein; p53 Protein; Phosphatase; Phosphorylation; Phosphoserine - metabolism; Polo-Like Kinase 1; Proteasome Endopeptidase Complex - metabolism; Proteasomes; Protein Binding; Protein Interaction Domains and Motifs; Protein Serine-Threonine Kinases - metabolism; Proteins; Proteolysis; Proto-Oncogene Proteins - metabolism; Research and Analysis Methods; Signal Transduction; Signaling; Transfection; Tumor proteins; Tumor Suppressor Protein p53 - metabolism; Ubiquitin; Ubiquitin - metabolism; Up-Regulation; Vero Cells; Vimentin; Vimentin - metabolism; Viral infections; Viral Proteins - chemistry; Viral Proteins - metabolism; Virus Replication; Viruses; United States > US; Taiwan

The p17 protein of avian reovirus (ARV) causes cell cycle retardation in a variety of cell lines; however, the underlying mechanism(s) by which p17 regulates the cell cycle remains largely unknown. We demonstrate for the first time that p17 interacts with CDK1 and vimentin as revealed by reciprocal...