Establishment of Hairy Root Cultures by Agrobacterium Rhizogenes Mediated Transformation of Isatis Tinctoria L. for the Efficient Production of Flavonoids and Evaluation of Antioxidant Activities

• Published in: PloS one Vol. 10; no. 3; p. e0119022
• Main Authors: Gai, Qing-Yan, Jiao, Jiao, Luo, Meng, Wei, Zuo-Fu, Zu, Yuan-Gang, Ma, Wei, Fu, Yu-Jie
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 18.03.2015; Public Library of Science (PLoS)
• Subjects: Agrobacterium - genetics; Agrobacterium - metabolism; Analysis; Antioxidants; Antioxidants (Nutrients); Antioxidants - chemistry; Antioxidants - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; beta-Glucosidase - genetics; beta-Glucosidase - metabolism; Biphenyl Compounds - antagonists & inhibitors; Biphenyl Compounds - metabolism; Carrier Proteins - genetics; Carrier Proteins - metabolism; Chinese medicine; Collaboration; Culture Media - chemistry; Culture Media - pharmacology; Design optimization; Factor Analysis, Statistical; Flavonoids; Flavonoids - biosynthesis; Flavonoids - chemistry; Food; Forestry; Gene Expression; Genetic transformation; Hairy root; Isatis - genetics; Isatis - metabolism; Isatis - microbiology; Isatis tinctoria; Isoflavones; Kaempferol; Liquiritigenin; Metabolites; Old fields; Pharmaceuticals; Phytochemicals; Picrates - antagonists & inhibitors; Picrates - metabolism; Plant ecology; Plant Roots - genetics; Plant Roots - metabolism; Plant Roots - microbiology; Plants, Genetically Modified; Quercetin; Roots; Rutin; Seeds; Tandem Mass Spectrometry; Tissue Culture Techniques - methods; Traditional Chinese medicine; Transformation; Transformation, Genetic; Beijing China; China

In this work, Isatis tinctoria hairy root cultures (ITHRCs) were established as an alternative source for flavonoids (FL) production. I. tinctoria hairy root line V was found to be the most efficient line and was further confirmed by the PCR amplification of rolB, rolC and aux1 genes. Culture parame...