Frequent arousal from hibernation linked to severity of infection and mortality in bats with white-nose syndrome

• Published in: PloS one Vol. 7; no. 6; p. e38920
• Main Authors: Reeder, DeeAnn M, Frank, Craig L, Turner, Gregory G, Meteyer, Carol U, Kurta, Allen, Britzke, Eric R, Vodzak, Megan E, Darling, Scott R, Stihler, Craig W, Hicks, Alan C, Jacob, Roymon, Grieneisen, Laura E, Brownlee, Sarah A, Muller, Laura K, Blehert, David S
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 20.06.2012; Public Library of Science (PLoS)
• Subjects: Animal behavior; Animal diseases; Animals; Arousal; Ascomycota - pathogenicity; Bats; Biology; Body fat; Body temperature; Body temperatures; Chiroptera; Chiroptera - microbiology; Chiroptera - physiology; Deoxyribonucleic acid; DNA; Ecology; Energy; Female; Fungi; Geology; Geomyces destructans; Hibernation; Hibernation - physiology; Immune system; Infections; Infectious diseases; Laboratories; Lasiurus borealis; Male; Mammals; Metabolism; Microclimate; Molossidae; Mortality; Mycoses; Myotis lucifugus; Nose - microbiology; Pest control; Skin; Skin - microbiology; Skin temperature; Studies; Temperature; Temperature data; Torpor; Trends; Vespertilionidae; Veterinary Science; White-nose syndrome; Winter; Vermont; West Virginia; New York; United States > US; Wisconsin; Pennsylvania; Michigan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0038920

White-nose syndrome (WNS), an emerging infectious disease that has killed over 5.5 million hibernating bats, is named for the causative agent, a white fungus (Geomyces destructans (Gd)) that invades the skin of torpid bats. During hibernation, arousals to warm (euthermic) body temperatures are normal but deplete fat stores. Temperature-sensitive dataloggers were attached to the backs of 504 free-ranging little brown bats (Myotis lucifugus) in hibernacula located throughout the northeastern USA. Dataloggers were retrieved at the end of the hibernation season and complete profiles of skin temperature data were available from 83 bats, which were categorized as: (1) unaffected, (2) WNS-affected but alive at time of datalogger removal, or (3) WNS-affected but found dead at time of datalogger removal. Histological confirmation of WNS severity (as indexed by degree of fungal infection) as well as confirmation of presence/absence of DNA from Gd by PCR was determined for 26 animals. We demonstrated that WNS-affected bats aroused to euthermic body temperatures more frequently than unaffected bats, likely contributing to subsequent mortality. Within the subset of WNS-affected bats that were found dead at the time of datalogger removal, the number of arousal bouts since datalogger attachment significantly predicted date of death. Additionally, the severity of cutaneous Gd infection correlated with the number of arousal episodes from torpor during hibernation. Thus, increased frequency of arousal from torpor likely contributes to WNS-associated mortality, but the question of how Gd infection induces increased arousals remains unanswered.