Sustained Release of BMP-2 in Bioprinted Alginate for Osteogenicity in Mice and Rats

• Published in: PloS one Vol. 8; no. 8; p. e72610
• Main Authors: Poldervaart, Michelle T., Wang, Huanan, van der Stok, Johan, Weinans, Harrie, Leeuwenburgh, Sander C. G., Öner, F. Cumhur, Dhert, Wouter J. A., Alblas, Jacqueline
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.08.2013; Public Library of Science (PLoS)
• Subjects: 3D printing; Alginates - pharmacology; Alginic acid; Animals; Biocompatibility; Biodegradation; Biological activity; Biological properties; Biology; Biomechanics; Biomedical materials; Bioprinting - methods; Bone growth; Bone morphogenetic protein 2; Bone Morphogenetic Protein 2 - metabolism; Bone morphogenetic proteins; Calcium; Calcium phosphate; Calcium phosphates; Cell Differentiation - drug effects; Comparative analysis; Controlled release; Delayed-Action Preparations; Differentiation; Drug dosages; Engineering; Female; Fractures; Gelatin; Gelatin - pharmacology; Glucuronic Acid - pharmacology; Goats; Growth factors; Hexuronic Acids - pharmacology; Humans; Hydrogels; Hydroxyapatites - pharmacology; Implantation; Kinetics; Materials Science; Medicine; Mice, Nude; Microparticles; Microspheres; Multipotent Stem Cells - cytology; Multipotent Stem Cells - drug effects; Multipotent Stem Cells - metabolism; Osteocalcin - metabolism; Osteogenesis; Osteogenesis - drug effects; Phosphates; Polymers; Printing; Product introduction; Proteins; Rats; Rats, Wistar; Scaffolds; Stromal cells; Stromal Cells - cytology; Stromal Cells - drug effects; Stromal Cells - metabolism; Subcutaneous Tissue - metabolism; Surgical implants; Sustained release; Three dimensional printing; Tissue engineering; Tissue Scaffolds - chemistry; Vehicles; Netherlands

The design of bioactive three-dimensional (3D) scaffolds is a major focus in bone tissue engineering. Incorporation of growth factors into bioprinted scaffolds offers many new possibilities regarding both biological and architectural properties of the scaffolds. This study investigates whether the s...