Novel organ-specific genetic factors for quantitative resistance to late blight in potato

• Published in: PloS one Vol. 14; no. 7; p. e0213818
• Main Authors: Juyo Rojas, Deissy Katherine, Soto Sedano, Johana Carolina, Ballvora, Agim, Léon, Jens, Mosquera Vásquez, Teresa
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.07.2019; Public Library of Science (PLoS)
• Subjects: Annotations; Biology and Life Sciences; Blight; Chromosome Mapping; Corn; Crop diseases; Crop science; Deoxyribonucleic acid; Disease control; Disease Resistance; DNA; DNA sequencing; Food security; Gene loci; Gene sequencing; Genes; Genetic aspects; Genetic factors; Genome-wide association studies; Genome-Wide Association Study; Genomes; Genomics; Genotype & phenotype; Genotyping; Kinases; Late blight; Leucine; Phenotypic variations; Phytophthora infestans - isolation & purification; Plant Diseases - genetics; Plant Diseases - parasitology; Plant Proteins - genetics; Polymorphism, Single Nucleotide; Potatoes; Proteins; Quantitative genetics; Quantitative Trait Loci; Research and Analysis Methods; Resistance factors; Single-nucleotide polymorphism; Solanum tuberosum - genetics; Colombia; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0213818

Potato, Solanum tuberosum, is one of the major consumed food in the world, being the basis of the diet of millions of people. The main limiting and destructive disease of potato is late blight, caused by Phytophtora infestans. Here, we present a multi-environmental analysis of the response to P. infestans using an association panel of 150 accessions of S. tuberosum Group Phureja, evaluated in two localities in Colombia. Disease resistance data were merged with a genotyping matrix of 83,862 SNPs obtained by 2b-restriction site-associated DNA and Genotyping by sequencing approaches into a Genome-wide association study. We are reporting 16 organ-specific QTL conferring resistance to late blight. These QTL explain between 13.7% and 50.9% of the phenotypic variance. Six and ten QTL were detected for resistance response in leaves and stem, respectively. In silico analysis revealed 15 candidate genes for resistance to late blight. Four of them have no functional genome annotation, while eleven candidate genes code for diverse proteins, including a leucine-rich repeat kinase.