A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against Plasmodium vivax antigens in a multiplexed bead-based assay using Luminex technology (Bio-Plex 200 or MAGPIX)

• Published in: PloS one Vol. 15; no. 12; p. e0238010
• Main Authors: Mazhari, Ramin, Brewster, Jessica, Fong, Rich, Bourke, Caitlin, Liu, Zoe S. J., Takashima, Eizo, Tsuboi, Takafumi, Tham, Wai-Hong, Harbers, Matthias, Chitnis, Chetan, Healer, Julie, Ome-Kaius, Maria, Sattabongkot, Jetsumon, Kazura, James, Robinson, Leanne J., King, Christopher, Mueller, Ivo, Longley, Rhea J.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 04.12.2020; Public Library of Science (PLoS)
• Subjects: Antibodies; Antigens; Antigens, Protozoan - immunology; Beads; Biology; Biology and Life Sciences; Cell Separation - methods; Child; Child, Preschool; Cohort analysis; Comparative analysis; COVID-19; Diagnosis; Engineering and Technology; Ethics; Female; Funding; Health aspects; Humans; Identification and classification; IgG antibody; Immunoassay; Immunoglobulin G; Immunoglobulin G - immunology; Immunologic tests; Immunomagnetic Separation - methods; Laboratories; Magnetic materials; Magnetic Phenomena; Malaria; Malaria - immunology; Malaria, Vivax - immunology; Male; Measuring instruments; Medical research; Medicine and Health Sciences; Methods; Microspheres; Multiplexing; Papua New Guinea - epidemiology; Parasites; Pediatrics; Plasma; Plasmodium vivax; Plasmodium vivax - immunology; Population; Proteins; Protozoan Proteins - immunology; Research and Analysis Methods; Severe acute respiratory syndrome coronavirus 2; Supervision; Technology; Vector-borne diseases; Vectors (Biology); Papua New Guinea; Thailand; United States > US; Japan; Victoria Australia; Ohio; France; Australia; Solomon Islands

Multiplexed bead-based assays that use Luminex ® xMAP ® technology have become popular for measuring antibodies against proteins of interest in many fields, including malaria and more recently SARS-CoV-2/COVID-19. There are currently two formats that are widely used: non-magnetic beads or magnetic b...