The Molecular Engineering of an Anti-Idiotypic Antibody for Pharmacokinetic Analysis of a Fully Human Anti-Infective

• Published in: PloS one Vol. 10; no. 12; p. e0145381
• Main Authors: Lim, She Yah, Chan, Conrad E. Z., Lisowska, Malgorzata M., Hanson, Brendon J., MacAry, Paul A.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 23.12.2015; Public Library of Science (PLoS)
• Subjects: Animals; Antibodies, Anti-Idiotypic - chemistry; Antibodies, Anti-Idiotypic - immunology; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - pharmacokinetics; Antibodies, Viral - chemistry; Antibodies, Viral - pharmacology; Antibody Affinity; Antibody Specificity; Antigens; Binding; Biomedical engineering; Biosafety; Cell Surface Display Techniques; Cercopithecus aethiops; Dengue; Dengue fever; Dengue Virus - immunology; Drug Evaluation, Preclinical - methods; Engineering; Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulin Fab Fragments - immunology; Immunoglobulins; Immunology; Laboratories; Life sciences; Methods; Mice; Monoclonal antibodies; Panning; Pathogens; Phages; Pharmacokinetics; Pharmacological research; Pharmacology; Polyethylene glycol; Proteins; Reagents; Vector-borne diseases; Vero Cells; Viral diseases; Viruses; United States > US; Singapore

Anti-idiotype monoclonal antibodies represent a class of reagents that are potentially optimal for analyzing the pharmacokinetics of fully human, anti-infective antibodies that have been developed as therapeutic candidates. This is particularly important where direct pathogen binding assays are comp...