Production of fluorescent antibody-labeling proteins in plants using a viral vector and the application in the detection of Acidovorax citrulli and Bamboo mosaic virus

• Published in: PloS one Vol. 13; no. 2; p. e0192455
• Main Authors: Kuo, Song-Yi, Lin, Yuan-Chuen, Lai, Yi-Chin, Liao, Jia-Teh, Hsu, Yau-Heiu, Huang, Hsiou-Chen, Hu, Chung-Chi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 06.02.2018; Public Library of Science (PLoS)
• Subjects: Acidovorax citrulli; Antibodies; Antigens; Assaying; Bacteria; Bamboo; Biology and Life Sciences; Biotechnology; Blotting, Western; Burkholderia; Chenopodium - metabolism; Chenopodium - virology; Coat protein; Comamonadaceae - genetics; Comamonadaceae - isolation & purification; Disease transmission; Electron microscopy; Fluorescence; Fluorescent Antibody Technique - methods; Genetic aspects; Genetic Vectors; Immunization; Immunoglobulin G; Immunoglobulins; Labeling; Labelling; Leaves; Ligands; Medicine and Health Sciences; Methods; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Pathogens; Plant diseases; Plant extracts; Plant Leaves - metabolism; Plant Proteins - metabolism; Plants - metabolism; Polymerase Chain Reaction; Potexvirus - genetics; Potexvirus - isolation & purification; Potexviruses; Protein A; Proteins; Quinoa; Rabbits; Research and Analysis Methods; Staphylococcus aureus; Transmission electron microscopy; Vectors (Biology); Virus-like particles; Viruses; Workers; Taiwan

Serological methods are relatively convenient and simple for the detection of pathogens for front-line workers. On-site visualization of the test results plays a pivotal role in the process. However, an efficient, universal labeling agent for antibodies is needed for the development of efficient ser...