Miniaturization and optimization of 384-well compatible RNA sequencing library preparation

• Published in: PloS one Vol. 14; no. 1; p. e0206194
• Main Authors: Mayday, Madeline Y., Khan, Lillian M., Chow, Eric D., Zinter, Matt S., DeRisi, Joseph L.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 10.01.2019; Public Library of Science (PLoS)
• Subjects: Acoustics; Automation; Automation, Laboratory; Biochemistry; Biology and life sciences; Biophysics; Business metrics; Cell culture; Chemical reduction; Consortia; Cost Savings; Critical care; Deoxyribonucleic acid; DNA; DNA methylation; DNA sequencing; Feasibility Studies; Gene sequencing; Genomes; Genomics; High-Throughput Nucleotide Sequencing - economics; High-Throughput Nucleotide Sequencing - instrumentation; High-Throughput Nucleotide Sequencing - methods; Human papillomavirus; Human wastes; Laboratories; Libraries; Library collections; Medicine; Medicine and Health Sciences; Metagenomics - economics; Metagenomics - instrumentation; Metagenomics - methods; Methods; Microchemistry - economics; Microchemistry - instrumentation; Microchemistry - methods; Microorganisms; Miniaturization; Next-generation sequencing; Optimization; Pathogens; Pediatrics; Reagents; Research and analysis methods; Ribonucleic acid; RNA; Sequence Analysis, RNA - economics; Sequence Analysis, RNA - instrumentation; Sequence Analysis, RNA - methods; Tips; United States > US; San Francisco California; California

Preparation of high-quality sequencing libraries is a costly and time-consuming component of metagenomic next generation sequencing (mNGS). While the overall cost of sequencing has dropped significantly over recent years, the reagents needed to prepare sequencing samples are likely to become the dom...