AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach
Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-dependent cloning methods for reasons of efficiency and performance. Here, we describe AQUA (advanced quick assembly), a simple and versatile seamless assembly cloning approach. We demonstrate the applicability...
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| Published in | PloS one Vol. 10; no. 9; p. e0137652 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Public Library of Science
11.09.2015
Public Library of Science (PLoS) |
| Subjects | |
| Online Access | Get full text |
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| Abstract | Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-dependent cloning methods for reasons of efficiency and performance. Here, we describe AQUA (advanced quick assembly), a simple and versatile seamless assembly cloning approach. We demonstrate the applicability and versatility of AQUA Cloning in selected proof-of-principle applications including targeted insertion-, deletion- and site-directed point-mutagenesis, and combinatorial cloning. Furthermore, we show the one pot de novo assembly of multiple DNA fragments into a single circular plasmid encoding a complex light- and chemically-regulated Boolean A NIMPLY B logic operation. AQUA Cloning harnesses intrinsic in vivo processing of linear DNA fragments with short regions of homology of 16 to 32 bp mediated by Escherichia coli. It does not require any kits, enzymes or preparations of reagents and is the simplest assembly cloning protocol to date. |
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| AbstractList | Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-dependent cloning methods for reasons of efficiency and performance. Here, we describe AQUA (
a
dvanced
qu
ick
a
ssembly), a simple and versatile seamless assembly cloning approach. We demonstrate the applicability and versatility of AQUA Cloning in selected proof-of-principle applications including targeted insertion-, deletion- and site-directed point-mutagenesis, and combinatorial cloning. Furthermore, we show the one pot
de novo
assembly of multiple DNA fragments into a single circular plasmid encoding a complex light- and chemically-regulated Boolean A NIMPLY B logic operation. AQUA Cloning harnesses intrinsic
in vivo
processing of linear DNA fragments with short regions of homology of 16 to 32 bp mediated by
Escherichia coli
. It does not require any kits, enzymes or preparations of reagents and is the simplest assembly cloning protocol to date. Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-dependent cloning methods for reasons of efficiency and performance. Here, we describe AQUA (advanced quick assembly), a simple and versatile seamless assembly cloning approach. We demonstrate the applicability and versatility of AQUA Cloning in selected proof-of-principle applications including targeted insertion-, deletion- and site-directed point-mutagenesis, and combinatorial cloning. Furthermore, we show the one pot de novo assembly of multiple DNA fragments into a single circular plasmid encoding a complex light- and chemically-regulated Boolean A NIMPLY B logic operation. AQUA Cloning harnesses intrinsic in vivo processing of linear DNA fragments with short regions of homology of 16 to 32 bp mediated by Escherichia coli. It does not require any kits, enzymes or preparations of reagents and is the simplest assembly cloning protocol to date. |
| Audience | Academic |
| Author | Gonschorek, Patrick Zurbriggen, Matias D Beyer, Hannes M Meier, Matthias Samodelov, Sophia L Weber, Wilfried |
| AuthorAffiliation | 1 Faculty of Biology, University of Freiburg, Freiburg, Germany 3 BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany 4 IMTEK, Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany Indian Institute of Science, INDIA 2 Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany |
| AuthorAffiliation_xml | – name: 2 Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany – name: 3 BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany – name: 1 Faculty of Biology, University of Freiburg, Freiburg, Germany – name: 4 IMTEK, Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany – name: Indian Institute of Science, INDIA |
| Author_xml | – sequence: 1 givenname: Hannes M surname: Beyer fullname: Beyer, Hannes M organization: Faculty of Biology, University of Freiburg, Freiburg, Germany; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany – sequence: 2 givenname: Patrick surname: Gonschorek fullname: Gonschorek, Patrick organization: Faculty of Biology, University of Freiburg, Freiburg, Germany – sequence: 3 givenname: Sophia L surname: Samodelov fullname: Samodelov, Sophia L organization: Faculty of Biology, University of Freiburg, Freiburg, Germany; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany – sequence: 4 givenname: Matthias surname: Meier fullname: Meier, Matthias organization: BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany; IMTEK, Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany – sequence: 5 givenname: Wilfried surname: Weber fullname: Weber, Wilfried organization: Faculty of Biology, University of Freiburg, Freiburg, Germany; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany; BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany – sequence: 6 givenname: Matias D surname: Zurbriggen fullname: Zurbriggen, Matias D organization: Faculty of Biology, University of Freiburg, Freiburg, Germany; BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26360249$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1038/nature07616 10.1016/j.mimet.2013.11.013 10.2144/000113705 10.1631/jzus.B1100180 10.1038/nchembio.926 10.1021/sb300027y 10.1093/nar/gku1189 10.1093/nar/gkt915 10.1016/S0378-1119(01)00824-1 10.1093/nar/gkq179 10.1021/sb4001992 10.1104/pp.113.217661 10.1101/gad.189035.112 10.1038/nrg3094 10.1038/nprot.2010.181 10.1038/nprot.2014.145 10.1093/nar/18.20.6069 10.1038/nmeth.1318 10.1016/j.jbbm.2005.12.008 10.1093/nar/gkr1288 10.1073/pnas.95.4.1460 10.1093/nar/gkm041 10.1038/nrm3767 10.1038/nature11149 10.1093/nar/gkt340 10.1007/s12033-012-9597-5 10.1038/nrg2775 10.1093/nar/27.19.e26 10.2144/000113418 10.1093/nar/gnf112 10.1016/S0960-9822(02)00450-5 10.1038/srep02052 10.1371/journal.pone.0119221 |
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| Copyright | COPYRIGHT 2015 Public Library of Science 2015 Beyer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2015 Beyer et al 2015 Beyer et al |
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| Notes | Competing Interests: The authors have declared that no competing interests exist. Conceived and designed the experiments: HMB MM WW MDZ. Performed the experiments: HMB PG SLS. Analyzed the data: HMB PG MDZ. Contributed reagents/materials/analysis tools: MM WW MDZ. Wrote the paper: HMB MDZ. |
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| References | R Bin Yang (ref12) 2013; 55 D Tillett (ref10) 1999; 27 S Schlatter (ref19) 2002; 282 J Quan (ref7) 2011; 6 B Mitta (ref16) 2002; 30 S Chen (ref26) 2012; 1 TM Joska (ref13) 2014; 100 S Wend (ref18) 2013; 3 A Casini (ref34) 2014; 42 B Munteanu (ref20) 2012; 13 C Aslanidis (ref31) 1990; 18 AS Khalil (ref25) 2010; 11 F Van den Ent (ref5) 2006; 67 R Heim (ref22) 1996; 6 GM Church (ref2) 2014; 15 DG Gibson (ref3) 2009; 6 Y Xia (ref21) 2014; 43 C Villalobos (ref24) 2012; 8 Y Zhang (ref9) 2012; 40 DR Burrill (ref27) 2012; 26 A V Bryksin (ref30) 2010; 48 SC Sleight (ref6) 2010; 38 K Müller (ref17) 2013; 41 W Weber (ref1) 2012; 13 A Sarrion-Perdigones (ref33) 2013; 162 J SantaLucia (ref15) 1998; 95 AP Jacobus (ref14) 2015; 10 A Matsumoto (ref11) 2011; 51 M Tigges (ref28) 2009; 457 C Engler (ref32) 2013 JP Torella (ref23) 2014; 9 S De Kok (ref4) 2014; 3 S Ausländer (ref29) 2012; 487 J Bitinaite (ref8) 2007; 35 |
| References_xml | – volume: 457 start-page: 309 year: 2009 ident: ref28 article-title: A tunable synthetic mammalian oscillator publication-title: Nature doi: 10.1038/nature07616 contributor: fullname: M Tigges – volume: 100 start-page: 46 year: 2014 ident: ref13 article-title: A universal cloning method based on yeast homologous recombination that is simple, efficient, and versatile publication-title: J Microbiol Methods doi: 10.1016/j.mimet.2013.11.013 contributor: fullname: TM Joska – volume: 51 start-page: 55 year: 2011 ident: ref11 article-title: Self-assembly cloning: A rapid construction method for recombinant molecules from multiple fragments publication-title: Biotechniques doi: 10.2144/000113705 contributor: fullname: A Matsumoto – volume: 13 start-page: 244 year: 2012 ident: ref20 article-title: Improvement of PCR reaction conditions for site-directed mutagenesis of big plasmids publication-title: J Zhejiang Univ Sci B doi: 10.1631/jzus.B1100180 contributor: fullname: B Munteanu – volume: 8 start-page: 477 year: 2012 ident: ref24 article-title: A combinatorial TIR1/AFB–Aux/IAA co-receptor system for differential sensing of auxin publication-title: Nat Chem Biol doi: 10.1038/nchembio.926 contributor: fullname: C Villalobos – volume: 1 start-page: 284 year: 2012 ident: ref26 article-title: Automated Design of Genetic Toggle Switches with Predetermined Bistability publication-title: ACS Synth Biol doi: 10.1021/sb300027y contributor: fullname: S Chen – volume: 43 start-page: e12 year: 2014 ident: ref21 article-title: New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase for site-directed mutagenesis publication-title: Nucleic Acids Res doi: 10.1093/nar/gku1189 contributor: fullname: Y Xia – volume: 42 start-page: e7 year: 2014 ident: ref34 article-title: One-pot DNA construction for synthetic biology: the Modular Overlap-Directed Assembly with Linkers (MODAL) strategy publication-title: Nucleic Acids Res doi: 10.1093/nar/gkt915 contributor: fullname: A Casini – volume: 282 start-page: 19 year: 2002 ident: ref19 article-title: SAMY, a novel mammalian reporter gene derived from Bacillus stearothermophilus alpha-amylase publication-title: Gene doi: 10.1016/S0378-1119(01)00824-1 contributor: fullname: S Schlatter – volume: 38 start-page: 2624 year: 2010 ident: ref6 article-title: In-Fusion BioBrick assembly and re-engineering publication-title: Nucleic Acids Res doi: 10.1093/nar/gkq179 contributor: fullname: SC Sleight – volume: 3 start-page: 97 year: 2014 ident: ref4 article-title: Rapid and reliable DNA assembly via ligase cycling reaction publication-title: ACS Synth Biol doi: 10.1021/sb4001992 contributor: fullname: S De Kok – volume: 162 start-page: 1618 year: 2013 ident: ref33 article-title: GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology publication-title: Plant Physiol doi: 10.1104/pp.113.217661 contributor: fullname: A Sarrion-Perdigones – volume: 26 start-page: 1486 year: 2012 ident: ref27 article-title: Synthetic memory circuits for tracking human cell fate publication-title: Genes Dev doi: 10.1101/gad.189035.112 contributor: fullname: DR Burrill – volume: 13 start-page: 21 year: 2012 ident: ref1 article-title: Emerging biomedical applications of synthetic biology publication-title: Nat Rev Genet doi: 10.1038/nrg3094 contributor: fullname: W Weber – volume: 6 start-page: 242 year: 2011 ident: ref7 article-title: Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries publication-title: Nat Protoc doi: 10.1038/nprot.2010.181 contributor: fullname: J Quan – volume: 9 start-page: 2075 year: 2014 ident: ref23 article-title: Unique nucleotide sequence-guided assembly of repetitive DNA parts for synthetic biology applications publication-title: Nat Protoc doi: 10.1038/nprot.2014.145 contributor: fullname: JP Torella – volume: 18 start-page: 6069 year: 1990 ident: ref31 article-title: Ligation-independent cloning of PCR products (LIC-PCR) publication-title: Nucleic Acids Res doi: 10.1093/nar/18.20.6069 contributor: fullname: C Aslanidis – volume: 6 start-page: 12 year: 2009 ident: ref3 article-title: Enzymatic assembly of DNA molecules up to several hundred kilobases publication-title: Nat Methods doi: 10.1038/nmeth.1318 contributor: fullname: DG Gibson – volume: 67 start-page: 67 year: 2006 ident: ref5 article-title: RF cloning: a restriction-free method for inserting target genes into plasmids publication-title: J Biochem Biophys Methods doi: 10.1016/j.jbbm.2005.12.008 contributor: fullname: F Van den Ent – volume: 40 start-page: e55 year: 2012 ident: ref9 article-title: SLiCE: a novel bacterial cell extract-based DNA cloning method publication-title: Nucleic Acids Res doi: 10.1093/nar/gkr1288 contributor: fullname: Y Zhang – volume: 95 start-page: 1460 year: 1998 ident: ref15 article-title: A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.95.4.1460 contributor: fullname: J SantaLucia – volume: 35 start-page: 1992 year: 2007 ident: ref8 article-title: USER friendly DNA engineering and cloning method by uracil excision publication-title: Nucleic Acids Res doi: 10.1093/nar/gkm041 contributor: fullname: J Bitinaite – volume: 15 start-page: 289 year: 2014 ident: ref2 article-title: Realizing the potential of synthetic biology publication-title: Nat Rev Mol Cell Biol doi: 10.1038/nrm3767 contributor: fullname: GM Church – volume: 487 start-page: 123 year: 2012 ident: ref29 article-title: Programmable single-cell mammalian biocomputers publication-title: Nature doi: 10.1038/nature11149 contributor: fullname: S Ausländer – volume: 41 start-page: e124 year: 2013 ident: ref17 article-title: Multi-chromatic control of mammalian gene expression and signaling publication-title: Nucleic Acids Res doi: 10.1093/nar/gkt340 contributor: fullname: K Müller – volume: 55 start-page: 10 year: 2013 ident: ref12 article-title: A novel T-type overhangs improve the enzyme-free cloning of PCR products publication-title: Mol Biotechnol doi: 10.1007/s12033-012-9597-5 contributor: fullname: R Bin Yang – volume: 11 start-page: 367 year: 2010 ident: ref25 article-title: Synthetic biology: applications come of age publication-title: Nat Rev Genet doi: 10.1038/nrg2775 contributor: fullname: AS Khalil – volume: 27 start-page: e26 year: 1999 ident: ref10 article-title: Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites publication-title: Nucleic Acids Res doi: 10.1093/nar/27.19.e26 contributor: fullname: D Tillett – year: 2013 ident: ref32 article-title: A Golden Gate Modular Cloning Toolbox for Plants publication-title: ACS Synth Biol contributor: fullname: C Engler – volume: 48 start-page: 463 year: 2010 ident: ref30 article-title: Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids publication-title: Biotechniques doi: 10.2144/000113418 contributor: fullname: A V Bryksin – volume: 30 start-page: e113 year: 2002 ident: ref16 article-title: Advanced modular self-inactivating lentiviral expression vectors for multigene interventions in mammalian cells and in vivo transduction publication-title: Nucleic Acids Res doi: 10.1093/nar/gnf112 contributor: fullname: B Mitta – volume: 6 start-page: 178 year: 1996 ident: ref22 article-title: Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer publication-title: Curr Biol doi: 10.1016/S0960-9822(02)00450-5 contributor: fullname: R Heim – volume: 3 start-page: 2052 year: 2013 ident: ref18 article-title: A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics publication-title: Sci Rep doi: 10.1038/srep02052 contributor: fullname: S Wend – volume: 10 start-page: e0119221 year: 2015 ident: ref14 article-title: Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli publication-title: PLoS One doi: 10.1371/journal.pone.0119221 contributor: fullname: AP Jacobus |
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| SubjectTerms | Analysis Assembly Boolean algebra Clonal deletion Cloning Cloning, Molecular - methods Combinatorial analysis Deoxyribonucleic acid Distribution DNA DNA polymerase E coli Enzymes Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Fragments Gene deletion Gene Expression Genetic aspects Genetic engineering Harnesses Homology Insertion Mutagenesis Mutagenesis, Site-Directed Plasmids Plasmids - genetics Reagents Studies Synthetic biology |
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| Title | AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach |
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