Metabolic Conversion of Ceramides in HeLa Cells - A Cholesteryl Phosphocholine Delivery Approach

• Published in: PloS one Vol. 10; no. 11; p. e0143385
• Main Authors: Kjellberg, Matti A., Lönnfors, Max, Slotte, J. Peter, Mattjus, Peter
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 24.11.2015; Public Library of Science (PLoS)
• Subjects: Alcohol; Apoptosis; Biochemistry; Cell growth; Cell Survival - drug effects; Ceramides; Ceramides - chemistry; Ceramides - metabolism; Ceramides - pharmacology; Chains; Cholesterol Esters - chemistry; Conversion; Diastereomers; Dosage; Drug resistance; Engineering; Enzymes; HeLa Cells; Humans; Kinases; Lipids; Membranes; Metabolism; Phosphocholine; Phosphorylcholine - chemistry; Plant lipids; Precursors; Sphingomyelin; United States > US; Finland

Ceramides can be delivered to cultured cells without solvents in the form of complexes with cholesteryl phosphocholine. We have analysed the delivery of three different radiolabeled D-erythro-ceramides (C6-Cer, C10-Cer and C16-Cer) to HeLa cells, and followed their metabolism as well as the cell via...