A novel RP-HPLC method for quantification of cholinesterase activity in human blood: An application for assessing organophosphate and carbamate insecticide exposure

• Published in: PloS one Vol. 17; no. 12; p. e0279287
• Main Authors: Sinha, Sukesh Narayan, Ungarala, Ramakrishna, Kumar, Dileshwar, Sangaraju, Rajendra, Kumar, Sarita
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 30.12.2022; Public Library of Science (PLoS)
• Subjects: Acetates; Acetic acid; Acetylcholinesterase; Biology and Life Sciences; Blood; Blood levels; Carbamates; Cholinesterase; Cholinesterase Inhibitors; Chromatography; Chromatography, High Pressure Liquid; Enzymatic activity; Enzyme activity; Enzymes; Evaluation; Health aspects; High performance liquid chromatography; Humans; Insecticides; Insecticides - toxicity; Liquid chromatography; Medicine and Health Sciences; Mental health; Methods; Naphthol; Nervous system; Organophosphates; Parameter sensitivity; Pesticides; Physical Sciences; Poisoning; Reproducibility of Results; Research and Analysis Methods; Sensitivity; Spraying; Substrates; Ultraviolet radiation; India

Several methods have been reported to estimate Acetylcholinesterase (AChE) enzyme activity in blood samples. The Ellman assay is the most important among all but with several shortcomings, and there is a need to develop a method which is accurate, sensitive and quick for analyzing AChE. Therefore, w...