An Overrepresentation of High Frequencies in the Mouse Inferior Colliculus Supports the Processing of Ultrasonic Vocalizations

• Published in: PloS one Vol. 10; no. 8; p. e0133251
• Main Authors: Garcia-Lazaro, Jose A., Shepard, Kathryn N., Miranda, Jason A., Liu, Robert C., Lesica, Nicholas A.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 05.08.2015; Public Library of Science (PLoS)
• Subjects: Anesthesia; Animal models; Animal vocalizations; Animals; Audiometry; Auditory nerve; Auditory pathway; Auditory Pathways - physiology; Auditory Perception - physiology; Auditory system; Biology; Brain stem; Cochlea; Cochlear Nerve - physiology; Cochlear nuclei; Cochlear Nucleus - physiology; Colliculus; Communication; Evoked Potentials, Auditory, Brain Stem - physiology; High frequencies; Inferior Colliculi - physiology; Inferior colliculus; Information processing; Mesencephalon; Mice; Neurology; Neurosciences; Phenotyping; Sensitivity; Specialization; Ultrasonic Waves; Vocalization behavior; Vocalization, Animal - physiology; United States; Atlanta Georgia; Georgia; United Kingdom > UK; United States > US
• DOI: 10.1371/journal.pone.0133251
• ISSN: 1932-6203

Mice are of paramount importance in biomedical research and their vocalizations are a subject of interest for researchers across a wide range of health-related disciplines due to their increasingly important value as a phenotyping tool in models of neural, speech and language disorders. However, the mechanisms underlying the auditory processing of vocalizations in mice are not well understood. The mouse audiogram shows a peak in sensitivity at frequencies between 15-25 kHz, but weaker sensitivity for the higher ultrasonic frequencies at which they typically vocalize. To investigate the auditory processing of vocalizations in mice, we measured evoked potential, single-unit, and multi-unit responses to tones and vocalizations at three different stages along the auditory pathway: the auditory nerve and the cochlear nucleus in the periphery, and the inferior colliculus in the midbrain. Auditory brainstem response measurements suggested stronger responses in the midbrain relative to the periphery for frequencies higher than 32 kHz. This result was confirmed by single- and multi-unit recordings showing that high ultrasonic frequency tones and vocalizations elicited responses from only a small fraction of cells in the periphery, while a much larger fraction of cells responded in the inferior colliculus. These results suggest that the processing of communication calls in mice is supported by a specialization of the auditory system for high frequencies that emerges at central stations of the auditory pathway.