Context-dependent expression of a conditionally-inducible form of active Akt

• Published in: PloS one Vol. 13; no. 6; p. e0197899
• Main Authors: Park, Soyeon, Burke, Robert E., Kareva, Tatyana, Kholodilov, Nikolai, Aimé, Pascaline, Franke, Thomas F., Levy, Oren, Greene, Lloyd A.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.06.2018; Public Library of Science (PLoS)
• Subjects: AKT protein; AKT1 protein; Animal models; Animals; Antibiotics; Apoptosis; Biodegradation; Biological activity; Biology; Biology and Life Sciences; Brain; Brain - cytology; Brain - metabolism; Cell culture; Cell Death; Cell Proliferation; Cellular signal transduction; Deprivation; Dihydrofolate reductase; Dopamine receptors; E coli; Enzyme Induction - genetics; FOXO4 protein; Gene Expression; Growth factors; HEK293 Cells; Humans; Kinases; Ligands; Medicine and Health Sciences; Mesencephalon; Mice; Mitosis; Movement disorders; MPP; Mutation; Neostriatum; Neurodegenerative diseases; Neurology; Neurons; Neurons - cytology; Neurons - metabolism; Neuroprotection; Neurosciences; Parkinson's disease; Pathology; Phosphorylation; Protein Domains; Protein Engineering; Protein kinases; Proteins; Proto-Oncogene Proteins c-akt - biosynthesis; Proto-Oncogene Proteins c-akt - chemistry; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; Rats; Reagents; Research and Analysis Methods; Substantia nigra; Substrates; Transcription Factors - metabolism; Trimethoprim; New York; United States > US

Akt kinases are key signaling components in proliferation-competent and post-mitotic cells. Here, we sought to create a conditionally-inducible form of active Akt for both in vitro and in vivo applications. We fused a ligand-responsive Destabilizing Domain (DD) derived from E. coli dihydrofolate red...