Development and utilization of a surrogate SARS-CoV-2 viral neutralization assay to assess mRNA vaccine responses

• Published in: PloS one Vol. 17; no. 1; p. e0262657
• Main Authors: Wisnewski, Adam V., Liu, Jian, Lucas, Carolina, Klein, Jon, Iwasaki, Akiko, Cantley, Linda, Fazen, Louis, Campillo Luna, Julian, Slade, Martin, Redlich, Carrie A.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 18.01.2022; Public Library of Science (PLoS)
• Subjects: ACE2; Angiotensin; Angiotensin-converting enzyme 2; Angiotensin-Converting Enzyme 2 - chemistry; Angiotensin-Converting Enzyme 2 - immunology; Antibodies; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; Biology and life sciences; Biosafety; Competition; Consent; Coronaviruses; Correlation; COVID-19; COVID-19 - immunology; COVID-19 - prevention & control; COVID-19 vaccines; COVID-19 Vaccines - administration & dosage; COVID-19 Vaccines - immunology; Enzyme-Linked Immunosorbent Assay; Enzymes; Evaluation; Female; Health aspects; Humans; Immune response; Immune system; Immunity; Immunoassays; Infections; Internal medicine; Male; Medicine; Medicine and Health Sciences; mRNA; mRNA vaccines; mRNA Vaccines - administration & dosage; mRNA Vaccines - immunology; Neutralization; Neutralizing; Peptidyl-dipeptidase A; Phlebotomy; Proteins; Receptors; Regression analysis; Research and Analysis Methods; SARS-CoV-2 - immunology; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Spike protein; Testing; Vaccination; Vaccines; Vaccines, Synthetic - administration & dosage; Vaccines, Synthetic - immunology; Viral diseases; United States; United States > US; Connecticut

Tests for SARS-CoV-2 immunity are needed to help assess responses to vaccination, which can be heterogeneous and may wane over time. The plaque reduction neutralization test (PRNT) is considered the gold standard for measuring serum neutralizing antibodies but requires high level biosafety, live vir...