Semantic Assembly and Annotation of Draft RNAseq Transcripts without a Reference Genome

• Published in: PloS one Vol. 10; no. 9; p. e0138006
• Main Authors: Ptitsyn, Andrey, Temanni, Ramzi, Bouchard, Christelle, Anderson, Peter A V
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.09.2015; Public Library of Science (PLoS)
• Subjects: Algorithms; Annotations; Assembly; Bioinformatics; Biology; Clustering; Computer applications; Cyanea capillata; Data processing; Evolution; Fragmentation; Fragments; Freeware; Gene expression; Gene families; Gene mapping; Gene sequencing; Genes; Genome; Genomes; Genomics; Homology; Nervous system; Neurons; Nucleotide sequence; Organisms; Parallel processing; Physiology; Proteins; RNA - genetics; RNA sequencing; Scaffolding; Scyphozoa; Seeds; Sequence Analysis, RNA - methods; Transcriptome; Workflow; Workflow software; United States > US; Sarasota Florida; Qatar

Transcriptomes are one of the first sources of high-throughput genomic data that have benefitted from the introduction of Next-Gen Sequencing. As sequencing technology becomes more accessible, transcriptome sequencing is applicable to multiple organisms for which genome sequences are unavailable. Cu...