Serum microRNA signatures as "liquid biopsies" for interrogating hepatotoxic mechanisms and liver pathogenesis in human

• Published in: PloS one Vol. 12; no. 5; p. e0177928
• Main Authors: Krauskopf, Julian, de Kok, Theo M, Schomaker, Shelli J, Gosink, Mark, Burt, Deborah A, Chandler, Patricia, Warner, Roscoe L, Johnson, Kent J, Caiment, Florian, Kleinjans, Jos C, Aubrecht, Jiri
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 17.05.2017; Public Library of Science (PLoS)
• Subjects: 3-Aminobutyric acid; Acetaminophen; Adenylation; Adhesion; Adult; Age; Alanine; Alcohols; Alkaline phosphatase; Analgesics; Analysis; Antigen presentation; Arches; Aspartate aminotransferase; Beta cells; Bilirubin; Biological activity; Biology and life sciences; Biomarkers; Biomarkers - blood; Biopsy; Blood; Blood circulation; Brain; Brain injury; Cell death; Chemical and Drug Induced Liver Injury - genetics; Chemotaxis; Developmental stages; Diabetes; Diabetes mellitus; Diabetes Mellitus, Type 2 - genetics; Disease; Drug overdose; Family medical history; Female; Females; Gene expression; Gene Expression Profiling - methods; Gene Expression Regulation; Gene Regulatory Networks; Genetic Predisposition to Disease; Glucose; Health care; Heart; Heart diseases; Hepatitis; Hepatitis B - genetics; Hepatotoxicity; High-Throughput Nucleotide Sequencing - methods; Humans; Infections; Infectious diseases; Inhibition; Injuries; Insulin; Interferon; Kinases; Liver; Liver cirrhosis; Liver Cirrhosis - genetics; Male; Males; Medicine and Health Sciences; Metabolic disorders; Metabolism; Mice; MicroRNA; MicroRNAs; MicroRNAs - blood; Multiplexing; Nitrogen; Nucleotide sequence; Nucleotides; Overdose; Oxidative stress; Pathogenesis; Pathology; Physical Sciences; Pneumonia; Product safety; Proteins; R&D; Research & development; Research and analysis methods; Ribonucleic acid; RNA; Safety; Safety research; Signatures; Toxicity; Viruses; Ann Arbor Michigan; United States > US; Connecticut; Michigan

MicroRNAs (miRNAs) released into the peripheral circulation upon cellular injury have shown a promise as a new class of tissue-specific biomarkers. We were first to demonstrate that next-generation sequencing analysis of serum from human subjects with acetaminophen-induced liver injury revealed a sp...