Attenuation of progressive hearing loss in DBA/2J mice by reagents that affect epigenetic modifications is associated with up-regulation of the zinc importer Zip4

• Published in: PloS one Vol. 10; no. 4; p. e0124301
• Main Authors: Mutai, Hideki, Miya, Fuyuki, Fujii, Masato, Tsunoda, Tatsuhiko, Matsunaga, Tatsuo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.04.2015; Public Library of Science (PLoS)
• Subjects: Acids; Age; Analysis; Animals; Attenuation; Auditory system; Brain research; Brain stem; Catechin - analogs & derivatives; Catechin - pharmacology; Cation Transport Proteins - genetics; Cation Transport Proteins - metabolism; Cochlea; Cochlea - drug effects; Cochlea - metabolism; Cyanocobalamin; Deoxyribonucleic acid; DNA; DNA methylation; DNA methyltransferase; DNA microarrays; DNA probes; Down-Regulation - drug effects; Environmental factors; Epigenesis, Genetic; Epigenetic inheritance; Epigenetics; Epithelium; Evoked Potentials, Auditory, Brain Stem - drug effects; Folic acid; Gene expression; Hearing loss; Hearing Loss - chemically induced; Hearing Loss - metabolism; Hearing Loss - pathology; Hearing protection; Histone deacetylase; Immunohistochemistry; Investigations; Laboratories; Medical research; Methionine; Methionine - toxicity; Mice; Mice, Inbred DBA; Microscopy, Fluorescence; Oligonucleotide Array Sequence Analysis; Organ of Corti; Organ of Corti - metabolism; Polymerase chain reaction; Proteins; Reagents; Real-Time Polymerase Chain Reaction; Serum levels; Spiral ganglion; Spiral Ganglion - metabolism; Stem cells; Transferases; Up-regulation; Up-Regulation - drug effects; Valproic acid; Valproic Acid - toxicity; Vitamin B12; Zinc; Japan

Various factors that are important for proper hearing have been identified, including serum levels of zinc. Here we investigated whether epigenetic regulatory pathways, which can be modified by environmental factors, could modulate hearing. RT-PCR detected expression of genes encoding DNA methyltran...