Use of Sequenom sample ID Plus® SNP genotyping in identification of FFPE tumor samples

• Published in: PloS one Vol. 9; no. 2; p. e88163
• Main Authors: Miller, Jessica K, Buchner, Nicholas, Timms, Lee, Tam, Shirley, Luo, Xuemei, Brown, Andrew M K, Pasternack, Danielle, Bristow, Robert G, Fraser, Michael, Boutros, Paul C, McPherson, John D
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.02.2014; Public Library of Science (PLoS)
• Subjects: Amplification; Analysis; Base pairs; Biology; Biophysics; Biotechnology industries; Deoxyribonucleic acid; DNA; DNA sequencing; DNA, Neoplasm - genetics; DNA, Neoplasm - isolation & purification; Fixatives; Forensic science; Formaldehyde; Gene sequencing; Genetic Markers; Genetic testing; Genomes; Genotyping; Genotyping Techniques; Humans; Identification; Identification methods; Male; Medical research; Medicine; Microsatellite Repeats; Multiplexing; Paraffin; Paraffin Embedding; Polymerase chain reaction; Polymorphism, Single Nucleotide; Prostate cancer; Prostatic Neoplasms - diagnosis; Prostatic Neoplasms - genetics; Quality Control; Quality management; Quality standards; Sampling methods; Short tandem repeats; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Tissues; Tumors

Short tandem repeat (STR) analysis, such as the AmpFlSTR® Identifiler® Plus kit, is a standard, PCR-based human genotyping method used in the field of forensics. Misidentification of cell line and tissue DNA can be costly if not detected early; therefore it is necessary to have quality control measu...