Use of a High-Density Protein Microarray to Identify Autoantibodies in Subjects with Type 2 Diabetes Mellitus and an HLA Background Associated with Reduced Insulin Secretion

• Published in: PloS one Vol. 10; no. 11; p. e0143551
• Main Authors: Chang, Douglas C., Piaggi, Paolo, Hanson, Robert L., Knowler, William C., Bucci, John, Thio, Guene, Hohenadel, Maximilian G., Bogardus, Clifton, Krakoff, Jonathan
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 25.11.2015; Public Library of Science (PLoS)
• Subjects: Adaptive systems; Adolescent; Adult; Analysis; Antibodies; Antigens; Autoantibodies; Autoantibodies - immunology; Autoantigens; Autoimmune diseases; Bioindicators; Biomarkers; Cohort Studies; Diabetes; Diabetes mellitus; Diabetes Mellitus, Type 2 - diagnosis; Diabetes Mellitus, Type 2 - etiology; Diabetes Mellitus, Type 2 - metabolism; Drb1 protein; Drug development; Epidemiology; Female; Glucose; Glucose tolerance; Haplotypes; Histocompatibility antigen HLA; HLA antigens; HLA Antigens - genetics; HLA Antigens - metabolism; Humans; Immune system; Immunoglobulins; Immunological tolerance; Insulin; Insulin - metabolism; Insulin Secretion; Kidney diseases; Male; Metabolism; Middle Aged; Obesity; Pathogenesis; Polypeptides; PPAR gamma - metabolism; Protein Array Analysis; Protein arrays; Proteins; Sensitivity and Specificity; Type 2 diabetes; Ubiquitin-Conjugating Enzymes - metabolism; Young Adult; United States; United States > US; Arizona; California

New biomarkers for type 2 diabetes mellitus (T2DM) may aid diagnosis, drug development or clinical treatment. Evidence is increasing for the adaptive immune system's role in T2DM and suggests the presence of unidentified autoantibodies. While high-density protein microarrays have emerged as a u...