Frequent occurrence of T cell–mediated late reactions revealed by atopy patch testing with hypoallergenic rBet v 1 fragments

• Published in: Journal of allergy and clinical immunology Vol. 137; no. 2; pp. 601 - 609.e8
• Main Authors: Campana, Raffaela, Moritz, Katharina, Marth, Katharina, Neubauer, Angela, Huber, Hans, Henning, Rainer, Blatt, Katharina, Hoermann, Gregor, Brodie, Tess M., Kaider, Alexandra, Valent, Peter, Sallusto, Federica, Wöhrl, Stefan, Valenta, Rudolf
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2016; Elsevier Limited
• Subjects: Adult; allergen; Allergens - immunology; Allergies; Allergy; Allergy and Immunology; Antigens, Plant - immunology; Asthma; atopy patch testing; Betula - adverse effects; birch pollen allergy; CCR4; Clinical trials; cutaneous lymphocyte antigen; Cytokines; Cytokines - biosynthesis; Dermatitis, Atopic - diagnosis; Dermatitis, Atopic - immunology; Dermatitis, Atopic - metabolism; Female; Histamine Release; Humans; Hypersensitivity, Delayed - diagnosis; Hypersensitivity, Delayed - immunology; Hypersensitivity, Delayed - metabolism; Hypersensitivity, Immediate - diagnosis; Hypersensitivity, Immediate - immunology; Hypersensitivity, Immediate - metabolism; Inflammation; late-phase reaction; Lymphocyte Activation - immunology; Lymphocytes; Male; Patch Tests; Pollen - immunology; Quality of life; rBet v 1; rBet v 1 fragments; recombinant hypoallergens; Rhinitis, Allergic, Seasonal - diagnosis; Rhinitis, Allergic, Seasonal - immunology; specific immunotherapy; Studies; T-Cell Antigen Receptor Specificity - immunology; T-cell proliferation; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; Young Adult; Allergy; allergen; AD; APT; late-phase reaction; rBet v 1; FACS; MFI; rBet v 1 fragments; cutaneous lymphocyte antigen; SPT; atopy patch testing; CLA; recombinant hypoallergens; birch pollen allergy; specific immunotherapy; CCR4; T-cell proliferation; SIT; Atopic dermatitis; Mean fluorescence intensity; Skin prick test; Fluorescence-activated cell sorting; Allergen-specific immunotherapy; Atopy patch test

Late allergic reactions are common in the course of allergen-specific immunotherapy and even occur with allergy vaccines with reduced IgE reactivity. We sought to study atopy patch test (APT) reactions and T-cell responses to the recombinant birch pollen allergen Bet v 1 and recombinant hypoallergen...