Gene Mapping via Bulked Segregant RNA-Seq (BSR-Seq)

• Published in: PloS one Vol. 7; no. 5; p. e36406
• Main Authors: Liu, Sanzhen, Yeh, Cheng-Ting, Tang, Ho Man, Nettleton, Dan, Schnable, Patrick S.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07.05.2012; Public Library of Science (PLoS)
• Subjects: Alleles; Analysis; Apoptosis; Arabidopsis; Arabidopsis thaliana; Bayes Theorem; Bayesian analysis; Binding sites; Bioinformatics; Biology; Biosynthesis; Chromosome Mapping; Cloning; Cloning, Molecular; Corn; Deoxyribonucleic acid; DNA; DNA methylation; Empirical analysis; Epigenetics; Fatty acids; Fatty Acids - biosynthesis; Fatty Acids - genetics; Gene expression; Gene Expression Profiling; Gene mapping; Gene mutation; Genes; Genetic aspects; Genetic markers; Genomes; Genomics; Genotype & phenotype; GL3 gene; High-Throughput Nucleotide Sequencing; Identification; Loci; Mapping; Markers; Mutants; Mutation; Oryza; Plant Proteins - genetics; Polymorphism, Single Nucleotide; Ribonucleic acid; RNA; RNA sequencing; Seeds - genetics; Single-nucleotide polymorphism; Transcription (Genetics); Transcription factors; Transcription Factors - genetics; Transposons; Trends; Zea mays - genetics; United States > US; Iowa

Bulked segregant analysis (BSA) is an efficient method to rapidly and efficiently map genes responsible for mutant phenotypes. BSA requires access to quantitative genetic markers that are polymorphic in the mapping population. We have developed a modification of BSA (BSR-Seq) that makes use of RNA-S...