Customized Internal Reference Controls for Improved Assessment of Circulating MicroRNAs in Disease

• Published in: PloS one Vol. 10; no. 5; p. e0127443
• Main Authors: Schlosser, Kenny, McIntyre, Lauralyn A., White, R. James, Stewart, Duncan J.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.05.2015; Public Library of Science (PLoS)
• Subjects: Algorithms; Biomarkers; Cancer; Comparators; Consent; Control methods; Diagnostic software; Diagnostic systems; Disease control; Feasibility studies; Gene expression; Hypertension; Hypertension, Pulmonary - blood; Hypertension, Pulmonary - genetics; Medical prognosis; Medicine; MicroRNA; MicroRNAs; MicroRNAs - blood; MicroRNAs - genetics; miRNA; Patients; Plasma; Pulmonary hypertension; Real-Time Polymerase Chain Reaction - methods; Real-Time Polymerase Chain Reaction - standards; Reference Standards; Reverse Transcriptase Polymerase Chain Reaction - methods; Reverse Transcriptase Polymerase Chain Reaction - standards; Sepsis; Septic shock; Shock, Septic - blood; Shock, Septic - genetics

Altered levels of circulating extracellular miRNA in plasma and serum have shown promise as non-invasive biomarkers of disease. However, unlike the assessment of cellular miRNA levels for which there are accepted housekeeping genes, analogous reference controls for normalization of circulating miRNA...