Expression Profiling in Bemisia tabaci under Insecticide Treatment: Indicating the Necessity for Custom Reference Gene Selection

• Published in: PloS one Vol. 9; no. 1; p. e87514
• Main Authors: Liang, Pei, Guo, Yajie, Zhou, Xuguo, Gao, Xiwu
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 31.01.2014; Public Library of Science (PLoS)
• Subjects: Abiotic factors; Agriculture; Agrochemicals; Analysis; Animals; Aphidoidea; Bactrocera dorsalis; Bemisia tabaci; Bioassays; Biology; Cytochrome; Ecosystem components; Elongation; Gene expression; Gene Expression Profiling; Gene Expression Regulation - drug effects; Genes; Glutathione; Glutathione transferase; Glyceraldehyde-3-phosphate dehydrogenase; Hemiptera - genetics; Hemiptera - metabolism; Insect Proteins - biosynthesis; Insecticides; Insecticides - pharmacology; Myzus persicae; Pesticides; Pests; Phosphates; Rankings; Solanum melongena; Solenopsis invicta; Studies; Tubulin; Beijing China; China

Finding a suitable reference gene is the key for qRT-PCR analysis. However, none of the reference gene discovered thus far can be utilized universally under various biotic and abiotic experimental conditions. In this study, we further examine the stability of candidate reference genes under a single...