A portable, low-cost device for precise control of specimen temperature under stereomicroscopes
To facilitate precise and convenient control of biological sample temperature, we developed a low-cost device that can be used independently or with any stereomicroscope. The purpose of the device is to control the thermal environment during experimental intervals in which a specimen must be manipul...
Saved in:
| Published in | PloS one Vol. 15; no. 3; p. e0230241 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Public Library of Science
11.03.2020
Public Library of Science (PLoS) |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | To facilitate precise and convenient control of biological sample temperature, we developed a low-cost device that can be used independently or with any stereomicroscope. The purpose of the device is to control the thermal environment during experimental intervals in which a specimen must be manipulated outside of an incubator, e.g. for dissection or slide-mounting in preparation for imaging. Sample temperatures can be both cooled to below and heated to above room temperatures, and stably maintained at a precision of +/- 0.1˚C. To demonstrate the utility of this device, we report improved characterization of the penetrance of a short-acting temperature-sensitive allele in C. elegans embryos, and identification of the upper temperature threshold for embryonic viability for six Caenorhabditis species. By controlling the temperature environment even as a specimen is manipulated, this device offers consistency and flexibility, reduces environmental noise, and enables precision timing in experiments requiring temperature shifts. |
|---|---|
| AbstractList | To facilitate precise and convenient control of biological sample temperature, we developed a low-cost device that can be used independently or with any stereomicroscope. The purpose of the device is to control the thermal environment during experimental intervals in which a specimen must be manipulated outside of an incubator, e.g. for dissection or slide-mounting in preparation for imaging. Sample temperatures can be both cooled to below and heated to above room temperatures, and stably maintained at a precision of +/- 0.1˚C. To demonstrate the utility of this device, we report improved characterization of the penetrance of a short-acting temperature-sensitive allele in C. elegans embryos, and identification of the upper temperature threshold for embryonic viability for six Caenorhabditis species. By controlling the temperature environment even as a specimen is manipulated, this device offers consistency and flexibility, reduces environmental noise, and enables precision timing in experiments requiring temperature shifts. To facilitate precise and convenient control of biological sample temperature, we developed a low-cost device that can be used independently or with any stereomicroscope. The purpose of the device is to control the thermal environment during experimental intervals in which a specimen must be manipulated outside of an incubator, e.g. for dissection or slide-mounting in preparation for imaging. Sample temperatures can be both cooled to below and heated to above room temperatures, and stably maintained at a precision of +/- 0.1˚C. To demonstrate the utility of this device, we report improved characterization of the penetrance of a short-acting temperature-sensitive allele in C . elegans embryos, and identification of the upper temperature threshold for embryonic viability for six Caenorhabditis species. By controlling the temperature environment even as a specimen is manipulated, this device offers consistency and flexibility, reduces environmental noise, and enables precision timing in experiments requiring temperature shifts. To facilitate precise and convenient control of biological sample temperature, we developed a low-cost device that can be used independently or with any stereomicroscope. The purpose of the device is to control the thermal environment during experimental intervals in which a specimen must be manipulated outside of an incubator, e.g. for dissection or slide-mounting in preparation for imaging. Sample temperatures can be both cooled to below and heated to above room temperatures, and stably maintained at a precision of +/- 0.1°C. To demonstrate the utility of this device, we report improved characterization of the penetrance of a short-acting temperature-sensitive allele in C. elegans embryos, and identification of the upper temperature threshold for embryonic viability for six Caenorhabditis species. By controlling the temperature environment even as a specimen is manipulated, this device offers consistency and flexibility, reduces environmental noise, and enables precision timing in experiments requiring temperature shifts. |
| Audience | Academic |
| Author | Paaby, Annalise B Testa, Nicholas D Lu, Hang Zhan, Mei Kaul, Samiksha Le, Kim N |
| AuthorAffiliation | 1 School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, United States of America 2 Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States of America Centre National de la Recherche Scientifique & University of Nice Sophia-Antipolis, FRANCE 3 School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, United States of America |
| AuthorAffiliation_xml | – name: Centre National de la Recherche Scientifique & University of Nice Sophia-Antipolis, FRANCE – name: 3 School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, United States of America – name: 1 School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, United States of America – name: 2 Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States of America |
| Author_xml | – sequence: 1 givenname: Nicholas D surname: Testa fullname: Testa, Nicholas D organization: School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, United States of America – sequence: 2 givenname: Samiksha orcidid: 0000-0003-4420-9896 surname: Kaul fullname: Kaul, Samiksha organization: School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, United States of America – sequence: 3 givenname: Kim N surname: Le fullname: Le, Kim N organization: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States of America – sequence: 4 givenname: Mei surname: Zhan fullname: Zhan, Mei organization: Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States of America – sequence: 5 givenname: Hang surname: Lu fullname: Lu, Hang organization: School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, United States of America – sequence: 6 givenname: Annalise B orcidid: 0000-0003-1422-047X surname: Paaby fullname: Paaby, Annalise B organization: School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, United States of America |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32160236$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNk1uL1DAUx4usuBf9BqIFQRTsmEubNC_CsHgZWFjw9hrS9HSmQ9rUJF3125s63WUq-yB9aDn9nX_OOfmf8-Sktz0kyVOMVphy_HZvR9crsxpieIUIRSTHD5IzLCjJGEH05Oj7NDn3fo9QQUvGHiWnlGAWU9hZItfpYF1QlYE3qbE_M219SGu4aTWkjXXp4EC3HlJt--CsSW2T-iGGOujTAN0AToXRQTr2NbjUB3Bgu1Y767UdwD9OHjbKeHgyvy-Sbx_ef738lF1df9xcrq8yzQQJWZ5XqFZNLKmoRUmEEFyXLC-ninEjKOZNRVXFKSqhJA2rlMICGOeKck7ynF4kzw-6g7FezrPxklAe-2clJ5HYHIjaqr0cXNsp91ta1cq_Aeu2UrnQagNSCcRrTYBXCuV5IUSDUc1LDEXBRZ3zqPVuPm2sOqg1xNkosxBd_unbndzaG8kRK0pcRIFXs4CzP0bwQXat12CM6sGOh7o5zVk5dfbiH_T-7mZqq2IDbd_YeK6eROWaYY4RjmaI1OoeKj41xDuLRmraGF8kvF4kTC6AX2GrRu_l5svn_2evvy_Zl0fsDpQJO2_NGFrb-yWYH8DJUd5BczdkjOS0B7fTkNMeyHkPYtqz4wu6S7o1Pv0D750DUw |
| CitedBy_id | crossref_primary_10_1093_genetics_iyad191 |
| Cites_doi | 10.1098/rsif.2018.0304 10.1186/1741-7007-10-59 10.1186/1471-2148-11-157 10.1534/genetics.115.183392 10.1016/j.celrep.2015.01.006 10.1534/g3.115.021451 10.1016/0092-8674(94)90238-0 10.1007/978-0-387-21706-2 10.1371/journal.pgen.1004293 10.2307/1535428 10.1534/genetics.117.300179 10.1038/35040562 10.1242/jeb.075408 10.1101/gr.228902 10.1046/j.1525-142X.2003.03003.x 10.1016/j.ydbio.2012.11.034 10.1098/rsbl.2012.0334 10.1016/bs.mcb.2016.05.004 10.1111/j.1558-5646.2010.01110.x 10.1371/journal.pone.0016644 10.32614/CRAN.package.survminer 10.1016/j.devcel.2014.05.009 10.1016/B978-0-12-544172-8.00014-1 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2020 Public Library of Science 2020 Testa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2020 Testa et al 2020 Testa et al |
| Copyright_xml | – notice: COPYRIGHT 2020 Public Library of Science – notice: 2020 Testa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2020 Testa et al 2020 Testa et al |
| DBID | NPM AAYXX CITATION IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PIMPY PQEST PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 5PM DOA |
| DOI | 10.1371/journal.pone.0230241 |
| DatabaseName | PubMed CrossRef Gale_Opposing Viewpoints In Context Gale In Context: Science ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts ProQuest Nursing and Allied Health Journals Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection ProQuest_Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection ProQuest Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni Edition) ProQuest Central UK/Ireland Advanced Technologies & Aerospace Database (1962 - current) Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection AUTh Library subscriptions: ProQuest Central Technology Collection ProQuest Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Materials Science Collection ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection (Proquest) (PQ_SDU_P3) ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection Biological Sciences Agricultural Science Database Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection Publicly Available Content Database ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | PubMed CrossRef Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Biological Science Collection ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic Technology Collection Technology Research Database Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | Agricultural Science Database PubMed |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) Engineering |
| DocumentTitleAlternate | Temperature control device |
| EISSN | 1932-6203 |
| Editor | Braendle, Christian |
| Editor_xml | – sequence: 1 givenname: Christian surname: Braendle fullname: Braendle, Christian |
| EndPage | e0230241 |
| ExternalDocumentID | 2376206872 oai_doaj_org_article_a907dc2e7ba044599f10d781e5579d47 A617101193 10_1371_journal_pone_0230241 32160236 |
| Genre | Journal Article |
| GeographicLocations | France Atlanta Georgia United States--US Georgia |
| GeographicLocations_xml | – name: France – name: Atlanta Georgia – name: United States--US – name: Georgia |
| GrantInformation_xml | – fundername: NIGMS NIH HHS grantid: R35 GM119744 – fundername: ; grantid: R35 GM119744 |
| GroupedDBID | --- 123 29O 2WC 3V. 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ ABDBF ABIVO ABJCF ABUWG ACGFO ACIHN ACIWK ACPRK ADBBV ADRAZ AEAQA AENEX AFKRA AFPKN AFRAH AHMBA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BBORY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESTFP ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IHR IHW INH INR IOV IPNFZ IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ NPM O5R O5S OK1 P2P P62 PATMY PDBOC PIMPY PQQKQ PROAC PSQYO PTHSS PV9 PYCSY RIG RNS RPM RZL SV3 TR2 UKHRP WOQ WOW ~02 ~KM AAYXX CITATION 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO FR3 GNUQQ H94 K9. KL. M7N P64 PQEST PQUKI PRINS RC3 7X8 5PM - 02 AAPBV ABPTK ADACO BBAFP KM |
| ID | FETCH-LOGICAL-c692t-44b0daf2365d9829997c864805381f9317fb3ab7308e82f6baa19e677a3772443 |
| IEDL.DBID | RPM |
| ISSN | 1932-6203 |
| IngestDate | Fri Nov 26 17:13:19 EST 2021 Fri Jul 05 11:54:08 EDT 2024 Tue Sep 17 21:26:52 EDT 2024 Wed Jul 24 18:29:27 EDT 2024 Thu Oct 10 16:41:19 EDT 2024 Thu Feb 22 23:36:07 EST 2024 Fri Feb 02 04:15:15 EST 2024 Thu Aug 01 20:08:41 EDT 2024 Thu Aug 01 20:29:32 EDT 2024 Tue Aug 20 22:12:48 EDT 2024 Wed Aug 07 14:00:28 EDT 2024 Sat Sep 28 08:28:58 EDT 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Language | English |
| License | This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Creative Commons Attribution License |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c692t-44b0daf2365d9829997c864805381f9317fb3ab7308e82f6baa19e677a3772443 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist. |
| ORCID | 0000-0003-4420-9896 0000-0003-1422-047X |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065815/ |
| PMID | 32160236 |
| PQID | 2376206872 |
| PQPubID | 1436336 |
| PageCount | e0230241 |
| ParticipantIDs | plos_journals_2376206872 doaj_primary_oai_doaj_org_article_a907dc2e7ba044599f10d781e5579d47 pubmedcentral_primary_oai_pubmedcentral_nih_gov_7065815 proquest_miscellaneous_2376734684 proquest_journals_2376206872 gale_infotracmisc_A617101193 gale_infotracacademiconefile_A617101193 gale_incontextgauss_ISR_A617101193 gale_incontextgauss_IOV_A617101193 gale_healthsolutions_A617101193 crossref_primary_10_1371_journal_pone_0230241 pubmed_primary_32160236 |
| PublicationCentury | 2000 |
| PublicationDate | 2020-03-11 |
| PublicationDateYYYYMMDD | 2020-03-11 |
| PublicationDate_xml | – month: 03 year: 2020 text: 2020-03-11 day: 11 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: San Francisco – name: San Francisco, CA USA |
| PublicationTitle | PloS one |
| PublicationTitleAlternate | PLoS One |
| PublicationYear | 2020 |
| Publisher | Public Library of Science Public Library of Science (PLoS) |
| Publisher_xml | – name: Public Library of Science – name: Public Library of Science (PLoS) |
| References | T Davies (pone.0230241.ref018) 2017; 137 CA Mok (pone.0230241.ref020) 2017; 207 GW Stegeman (pone.0230241.ref027) 2013; 216 CC Mello (pone.0230241.ref016) 1994; 77 KG Hales (pone.0230241.ref004) 2015; 201 T Davies (pone.0230241.ref030) 2014; 30 D GuhaThakurta (pone.0230241.ref007) 2002; 12 AB Paaby (pone.0230241.ref022) 2018 SG Kuntz (pone.0230241.ref003) 2014; 10 FR Lillie (pone.0230241.ref001) 1897; 1 J Lowry (pone.0230241.ref019) 2015; 5 JL Richards (pone.0230241.ref029) 2013; 374 KN Le (pone.0230241.ref009) 2019 MA Felix (pone.0230241.ref023) 2012; 10 JL Anderson (pone.0230241.ref025) 2011; 11 A Prasad (pone.0230241.ref026) 2011; 65 pone.0230241.ref014 pone.0230241.ref013 J Chong (pone.0230241.ref002) 2018; 15 C Matsuba (pone.0230241.ref024) 2013; 9 T Stiernagle (pone.0230241.ref005) 2006 R Core Team (pone.0230241.ref012) 2017 MR Wallenfang (pone.0230241.ref017) 2000; 408 D Slos (pone.0230241.ref028) 2017; 2 K Kemphues (pone.0230241.ref008) 2005 SM O'Rourke (pone.0230241.ref010) 2011; 6 HF Nijhout (pone.0230241.ref021) 2003; 5 J Hardin (pone.0230241.ref011) 2011; 106 WN Venables (pone.0230241.ref015) 2002 ML Begasse (pone.0230241.ref006) 2015; 10 |
| References_xml | – volume: 15 issue: 144 year: 2018 ident: pone.0230241.ref002 article-title: Temporal development of Drosophila embryos is highly robust across a wide temperature range publication-title: Journal of the Royal Society, Interface doi: 10.1098/rsif.2018.0304 contributor: fullname: J Chong – volume: 2 issue: 4 year: 2017 ident: pone.0230241.ref028 article-title: Caenorhabditis monodelphis sp. n.: defining the stem morphology and genomics of the genus Caenorhabditis publication-title: BMC Zoology contributor: fullname: D Slos – volume: 10 start-page: 59 year: 2012 ident: pone.0230241.ref023 article-title: Population dynamics and habitat sharing of natural populations of Caenorhabditis elegans and C. briggsae publication-title: BMC biology doi: 10.1186/1741-7007-10-59 contributor: fullname: MA Felix – volume: 11 start-page: 157 year: 2011 ident: pone.0230241.ref025 article-title: Does thermoregulatory behavior maximize reproductive fitness of natural isolates of Caenorhabditis elegans? publication-title: BMC evolutionary biology doi: 10.1186/1471-2148-11-157 contributor: fullname: JL Anderson – volume: 201 start-page: 815 issue: 3 year: 2015 ident: pone.0230241.ref004 article-title: Genetics on the Fly: A Primer on the Drosophila Model System publication-title: Genetics doi: 10.1534/genetics.115.183392 contributor: fullname: KG Hales – year: 2019 ident: pone.0230241.ref009 article-title: HeALTH: An Automated Platform for Long-term Longitudinal Studies of Whole Organisms under Precise Environmental Control. publication-title: bioRxiv contributor: fullname: KN Le – volume: 10 start-page: 647 issue: 5 year: 2015 ident: pone.0230241.ref006 article-title: Temperature Dependence of Cell Division Timing Accounts for a Shift in the Thermal Limits of C. elegans and C. briggsae publication-title: Cell reports doi: 10.1016/j.celrep.2015.01.006 contributor: fullname: ML Begasse – volume: 5 start-page: 2241 issue: 11 year: 2015 ident: pone.0230241.ref019 article-title: High-Throughput Cloning of Temperature-Sensitive Caenorhabditis elegans Mutants with Adult Syncytial Germline Membrane Architecture Defects. publication-title: G3 (Bethesda, Md) doi: 10.1534/g3.115.021451 contributor: fullname: J Lowry – volume-title: R: A language and environment for statistical computing year: 2017 ident: pone.0230241.ref012 contributor: fullname: R Core Team – volume: 77 start-page: 95 issue: 1 year: 1994 ident: pone.0230241.ref016 article-title: The maternal genes apx-1 and glp-1 and establishment of dorsal-ventral polarity in the early C. elegans embryo publication-title: Cell doi: 10.1016/0092-8674(94)90238-0 contributor: fullname: CC Mello – volume-title: Modern Applied Statistics with S year: 2002 ident: pone.0230241.ref015 doi: 10.1007/978-0-387-21706-2 contributor: fullname: WN Venables – volume-title: Evolutionary Developmental Biology: A Reference Guide year: 2018 ident: pone.0230241.ref022 contributor: fullname: AB Paaby – volume: 10 start-page: e1004293 issue: 4 year: 2014 ident: pone.0230241.ref003 article-title: Drosophila embryogenesis scales uniformly across temperature in developmentally diverse species publication-title: PLoS genetics doi: 10.1371/journal.pgen.1004293 contributor: fullname: SG Kuntz – volume: 1 start-page: 179 year: 1897 ident: pone.0230241.ref001 article-title: On the effect of temperature on the development of animals publication-title: Zoological Bulletin doi: 10.2307/1535428 contributor: fullname: FR Lillie – volume: 207 start-page: 447 issue: 2 year: 2017 ident: pone.0230241.ref020 article-title: MIP-MAP: High-Throughput Mapping of Caenorhabditis elegans Temperature-Sensitive Mutants via Molecular Inversion Probes publication-title: Genetics doi: 10.1534/genetics.117.300179 contributor: fullname: CA Mok – volume: 408 start-page: 89 issue: 6808 year: 2000 ident: pone.0230241.ref017 article-title: Polarization of the anterior-posterior axis of C. elegans is a microtubule-directed process publication-title: Nature doi: 10.1038/35040562 contributor: fullname: MR Wallenfang – volume: 216 start-page: 850 issue: 5 year: 2013 ident: pone.0230241.ref027 article-title: Temperature-dependent behaviours are genetically variable in the nematode Caenorhabditis briggsae publication-title: The Journal of experimental biology doi: 10.1242/jeb.075408 contributor: fullname: GW Stegeman – volume: 12 start-page: 701 issue: 5 year: 2002 ident: pone.0230241.ref007 article-title: Identification of a novel cis-regulatory element involved in the heat shock response in Caenorhabditis elegans using microarray gene expression and computational methods publication-title: Genome research doi: 10.1101/gr.228902 contributor: fullname: D GuhaThakurta – start-page: 1 year: 2005 ident: pone.0230241.ref008 article-title: Essential genes publication-title: WormBook: the online review of C elegans biology contributor: fullname: K Kemphues – ident: pone.0230241.ref013 – volume: 5 start-page: 9 issue: 1 year: 2003 ident: pone.0230241.ref021 article-title: Development and evolution of adaptive polyphenisms publication-title: Evolution & development doi: 10.1046/j.1525-142X.2003.03003.x contributor: fullname: HF Nijhout – volume: 374 start-page: 12 issue: 1 year: 2013 ident: pone.0230241.ref029 article-title: A quantitative model of normal Caenorhabditis elegans embryogenesis and its disruption after stress publication-title: Developmental biology doi: 10.1016/j.ydbio.2012.11.034 contributor: fullname: JL Richards – volume: 9 start-page: 20120334 issue: 1 year: 2013 ident: pone.0230241.ref024 article-title: Temperature, stress and spontaneous mutation in Caenorhabditis briggsae and Caenorhabditis elegans publication-title: Biology letters doi: 10.1098/rsbl.2012.0334 contributor: fullname: C Matsuba – volume: 137 start-page: 283 year: 2017 ident: pone.0230241.ref018 article-title: Using fast-acting temperature-sensitive mutants to study cell division in Caenorhabditis elegans publication-title: Methods in cell biology doi: 10.1016/bs.mcb.2016.05.004 contributor: fullname: T Davies – volume: 65 start-page: 52 issue: 1 year: 2011 ident: pone.0230241.ref026 article-title: Temperature-dependent fecundity associates with latitude in Caenorhabditis briggsae publication-title: Evolution; international journal of organic evolution doi: 10.1111/j.1558-5646.2010.01110.x contributor: fullname: A Prasad – volume: 6 start-page: e16644 issue: 3 year: 2011 ident: pone.0230241.ref010 article-title: A survey of new temperature-sensitive, embryonic-lethal mutations in C. elegans: 24 alleles of thirteen genes. publication-title: PloS one doi: 10.1371/journal.pone.0016644 contributor: fullname: SM O'Rourke – ident: pone.0230241.ref014 doi: 10.32614/CRAN.package.survminer – start-page: 1 year: 2006 ident: pone.0230241.ref005 article-title: Maintenance of C. elegans publication-title: WormBook: the online review of C elegans biology contributor: fullname: T Stiernagle – volume: 30 start-page: 209 issue: 2 year: 2014 ident: pone.0230241.ref030 article-title: High-resolution temporal analysis reveals a functional timeline for the molecular regulation of cytokinesis publication-title: Developmental cell doi: 10.1016/j.devcel.2014.05.009 contributor: fullname: T Davies – volume: 106 start-page: 377 year: 2011 ident: pone.0230241.ref011 article-title: Imaging embryonic morphogenesis in C. elegans publication-title: Methods in cell biology doi: 10.1016/B978-0-12-544172-8.00014-1 contributor: fullname: J Hardin |
| SSID | ssj0053866 |
| Score | 2.3756747 |
| Snippet | To facilitate precise and convenient control of biological sample temperature, we developed a low-cost device that can be used independently or with any... |
| SourceID | plos doaj pubmedcentral proquest gale crossref pubmed |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database |
| StartPage | e0230241 |
| SubjectTerms | Analysis Background noise Biological samples Biology and Life Sciences Biomedical engineering Characterization Copper Data collection Embryonic development Embryos Engineering Engineering and Technology Experiments Gene expression Heat LCDs Liquid crystal displays Low cost Microscopes Noise pollution Noise reduction Physical Sciences Portable equipment Power supply Research and Analysis Methods Room temperature Surgery Temperature requirements Testing Thermal environments Thermometers Viability |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELbQnrggyquBAgYhARJp40f8OC6IqiABElDUm2UnNiCtkqjZVf9-PbF31aBKcOAaT6JkHp7P8cxnhF5YBnlUi5K2vCl5U_lSB8JL1cR0bkVgVkCj8KfP4uSUfzyrz64c9QU1YYkeOCnuyMbVW9tQL52tOK91fFLVSkV8XUvd8tRHTurtYirNwTGKhciNckySo2yXw6Hv_CGgbsrJLBFNfP27WXkxrPrxOsj5Z-XklVR0fBvdyhgSL9O776EbvruD9nKUjvhVppJ-fReZJZ7wtVv5N3jVX5RNP65x62F6wBGu4gHILUaPc8U67gOG3kug_MfAWpUplzG0mp1jIFXw0MYMFJj94Md76PT4_fd3J2U-UqFshKbrknNXtTZQJupWq5iKtGyU4Ao0RoKOYCI4Zl0Me-UVDcJZS7QXUloWYTjn7D5adFGJ-whbTeLqtlWOhRCTGldW1tZrbqVwxFpXoHKrXzMk5gwzbZ_JuOJIijJgD5PtUaC3YISdLPBeTxeiN5jsDeZv3lCgp2BCk5pId9FrlhGoEaC3YwV6PkkA90UHxTU_7WYczYcvP_5B6NvXmdDLLBT66AyNzQ0N8ZuAU2smeTCTjBHczIb3weG2WhkNVCrRSihJ451bJ7x--NluGB4KBXOd7zdJRjIuFC_Qg-SzO80ySgQcHFAgOfPmmernI93vXxP1OGyKK1I__B-2eoRuUvh5AcWR5AAt1ucb_zgivLV7MgXzJSWDTQk priority: 102 providerName: Directory of Open Access Journals – databaseName: ProQuest Public Health Database dbid: 8C1 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Lb9QwELZgucAB0fJooIBBSICE23Xs-HFCS0VVkAAJKOrNshOnIK3isNkVfx9P4g0NqhDX9STKztv2zDcIPbMM4qgWJK94SXg590TXlBNVxnBuRc2sgEbhDx_FySl_f1acpQO3LpVVbn1i76irUMIZ-SFUb-RzoWT-uv1JYGoU3K6mERpX0TUaFRNGN6ijscQj2rIQqV2OSXqYpHPQhsYfQO6dczoJRz1q_-ibZ-0ydJclnn_XT14ISMe30M2USeLFIPoddMU3u-jGBXzBXbSTLLfDLxK89MvbyCxwn3O7pX-Fl-EXKUO3xpUHl4FjCotbALzoPE5V7DjUGPoxYQwABiSrBMOMof1shQFowUNrM8BihtZ3d9Dp8duvRyckjVkgpdD5mnDu5pWtcyaKSqsYnrQsleAK-EdrHROM2jHroitQXuW1cNZS7YWUlsXUnHN2F82ayNI9hK2mccdbKcfqOgY6rqwsrNfcSuGotS5DZMtt0w5oGqa_UpNxFzKwzYB0TJJOht6ASEZawMLufwirc5NMy9i4v6_K3Etn55wXOuravJKK-qKQuuIyQ49BoGZoLB0t2ixi8kYB8o5l6GlPAXgYDRTcnNtN15l3n779B9GXzxOi54moDlE1SpuaHOJ_ApytCeX-hDJadTlZ3gP123KlM3_0Pz65VcnLl5-My_BSKKJrfNgMNJJxoXiG7g0aPHKW5VTAMIEMyYluT1g_XWl-fO_hyOGiXNHi_r8_6wG6nsNRBZRC0n00W682_mHM59buUW-0vwFMeUli priority: 102 providerName: ProQuest – databaseName: Scholars Portal Journals: Open Access dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3di9QwEA_H-uKLeH5d9dQoggp2adI0Hw8iq3icwimoK_cW0jY9haVdt7uo_70zbVqsrOCDr51JofORmTQzvyHkkUsxjhoZ81IUsSgSH5uKiVgXEM6drFInsVH47J08XYq359n5ARlmtgYBtnuPdjhParlZzX98-_kCHP55N7VBsWHRfN3Ufo45NcdO9ktcpAJt_kyM9wrg3VKGBrq_rZwEqA7Hf9ytZ-tV0-5LRf-sqPwtRJ1cJVdCbkkXvTEckgNfXyOHwXtb-iRATD-9TuyCdp-cr_wzumq-x0XTbmnpcdugkMbSNYJetJ6GSnbaVBR7MnEUAEU0qwDFTLEFbUMRbMFjezNCY4L82htkefL606vTOIxaiAtp-DYWIk9KV_FUZqXREKKMKrQUGiXGKgNJRpWnLoftQHvNK5k7x4yXSrkU0nMh0ptkVoMQjwh1hsGpt9R5WlUQ7IR2KnPeCKdkzpzLIxIP8rXrHlHDdtdqCk4ivaAs6sMGfUTkJSph5EU87O5Bs7mwwb2sgzN-WXCvcpcIkRmwt6RUmvksU6YUKiL3UYW2by4dvdouIIFjCHuXRuRhx4GYGDUW3Vy4XdvaN-8__wPTxw8TpseBqWrAGAoXGh3gmxBra8J5POEEzy4m5CM0uEEqrcUKJp5IrTisHIxwP_nBSMaXYiFd7Ztdz6PAL7SIyK3eZkfJppxJHCgQETWx5onop5T665cOkhwvyzXLbv8PXd0hlzn-1MCiSXZMZtvNzt-FzG-b3-uc-RcvMled priority: 102 providerName: Scholars Portal |
| Title | A portable, low-cost device for precise control of specimen temperature under stereomicroscopes |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/32160236 https://www.proquest.com/docview/2376206872 https://search.proquest.com/docview/2376734684 https://pubmed.ncbi.nlm.nih.gov/PMC7065815 https://doaj.org/article/a907dc2e7ba044599f10d781e5579d47 http://dx.doi.org/10.1371/journal.pone.0230241 |
| Volume | 15 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3fb9MwELa2IqG9TIxfK4xiEBIgkbaOHdt57KqWgdQxDYb6FjmJMyZ1SdS04o2_nbvEqQjaA-LFD_ElSu7u852Tuy-EvDEc42goPT8ViSeSsfXCjAlPJxDOjcy4kdgovDiXZ1fi8zJY7pGg7YWpi_aT-GaYr26H-c2PurayvE1GbZ3Y6GIxxU9zmgWjfbKvOG-36M3yCwCW0vXIccVGziTDssjtEBNuCFkH5D73mUTy9E44qln7d2tzr1wV1V2J59_1k38EpPkDcugySTpp7viI7Nn8ITlyWK3oO0co_f4RiSa0zrLjlf1AV8VPLymqDU0tLhIUklZaIsVFZamrW6dFRrEDE4n_KXJXOeJlig1na4rUChabmZEIsyht9ZhczWffpmee-7GCl8jQ33hCxOPUZPD4QRpqCEihSrQUGpXHshBSiizmJgbwa6v9TMbGsNBKpQyHZFwI_oT0ctDnMaEmZLDHTXXMswxCm9BGBcaGwigZM2PiPvFa_UZlw58R1R_RFOw7GkVFaJrImaZPTtEIO1lkv64PFOvryPlAZGBHnya-VbEZCxGE4F3jVGlmg0CFqVB98hJNGDWtpDsMRxNI1xiS3PE-eV1LIANGjiU212ZbVdGnL9__QejrZUforRPKCnCGxLi2BngmZNbqSJ50JAHHSWf6GB2u1UoVYb2SP5Za-XBm64R3T7_aTeNFsWwut8W2kVFcSC365GnjszvNtgjoE9Xx5o7quzOAx5qA3OHv2X-f-Zwc-PjeAusi2QnpbdZb-wKSu008AEgvFYx6ynCcfxyQe6ez84vLQf26BMaF0Dj-mg1q4P8GrpxVNQ |
| link.rule.ids | 230,315,733,786,790,870,891,2115,2236,12083,12250,12792,21416,24346,27955,27956,31752,31753,33299,33300,33406,33407,33777,33778,43343,43612,43633,43838,53825,53827,74100,74369,74390,74657 |
| linkProvider | National Library of Medicine |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Lb9QwELZgewAOiJZHUwo1CAmQSBvHjh8ntEWtttAuqLRVb5aTOKXSKgmbXfH38STO0qAKcY0nUTLv2DPfIPTGUIijiodxzrKQZZENVUFYKDMXzg0vqOHQKHwy5ZNz9vkyufQbbo0vq-x9Yuuo8yqDPfI9qN6IIy5F_LH-GcLUKDhd9SM07qI1gNyUI7S2fzD9dtr7YmfNnPuGOSrInpfPbl2Vdhey75iRQUBqcftX3nlUz6rmttTz7wrKGyHp8BF66HNJPO6Ev47u2HIDPbiBMLiB1r3tNvidB5h-_xjpMW6z7nRmP-BZ9SvMqmaBcwtOA7skFtcAedFY7OvYcVVg6MiEQQAYsKw8EDOGBrQ5BqgFC83NAIxZ1bZ5gs4PD84-TUI_aCHMuIoXIWNplJsipjzJlXQBSolMciaBf6RQLsUoUmpS5wyklXHBU2OIslwIQ11yzhh9ikalY-kmwkYRJ4hcprQoXKhj0ojEWMWM4CkxJg1Q2HNb1x2ehm4P1YT7D-nYpkE62ksnQPsgkhUtoGG3F6r5lfbGpY37w8-z2IrURIwlymlblAtJbJIIlTMRoB0QqO5aS1c2rccufSMAekcD9LqlAESMEkpursyyafTR14v_IPp-OiB664mKyqlGZnybg_smQNoaUG4PKJ1dZ4PlTVC_niuN_mMB7s5eJW9ffrVahodCGV1pq2VHIyjjkgXoWafBK87SmHAYJxAgMdDtAeuHK-X1jxaQHI7KJUm2_v1aO-je5OzkWB8fTb88R_dj2LiAwkiyjUaL-dK-cNndIn3pTfg3BY1N9g |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1bb9MwFLagSAgeEBuXBQYzCAmQyBrHji9PqFzKxmUgYGhvlpPYA6lKQtOKv49P4oYFTYjX-qRqj8_lc3zOdxB6ZCjkUcXjtGRFzIrExsoRFsvCp3PDHTUcGoU_HPGDY_b2JDsJ9U9tKKvcxMQuUJd1Ae_Ip1C9kSZcinTqQlnEp1fz583PGCZIwU1rGKdxEV0CkA1jHOT8zSYqe7_mPLTOUUGmYaf2m7qy-4DDU0ZGqalj8B_i9KRZ1O15IPTvWsozyWl-HV0LqBLPejPYQhdstY2unuEa3EZbwYtb_CRQTT-9gfQMd_g7X9hneFH_iou6XeHSQvjAHs7iBsgvWotDRTuuHYbeTBgJgIHVKlAyY2hFW2IgXbDQ5gwUmXVj25voeP7668uDOIxciAuu0lXMWJ6UxqWUZ6WSPlUpUUjOJOiPOOXBhsupyX1YkFamjufGEGW5EIZ6mM4YvYUmlVfpDsJGEX_6LWVOnfNJj0kjMmMVM4LnxJg8QvFG27rpmTV0d70m_ImkV5uG3dFhdyL0ArZkkAVe7O6Denmqg5tp48_6ZZFakZuEsUx5u0tKIYnNMqFKJiK0Bxuq-ybTwbv1zAM5AvR3NEIPOwngxqjAyk7Num314cdv_yH05fNI6HEQcrU3jcKEhgf_n4BzayS5O5L0Hl6MlnfA_DZaafUfX_BPbkzy_OUHwzJ8KRTUVbZe9zKCMi5ZhG73FjxolqaEw2CBCImRbY9UP16pfnzvqMnh0lyS7M6_f9Yeuux9V78_PHp3F11J4Q0GVEiSXTRZLdf2nod5q_x-57-_ASyaULw |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+portable%2C+low-cost+device+for+precise+control+of+specimen+temperature+under+stereomicroscopes&rft.jtitle=PloS+one&rft.au=Nicholas+D+Testa&rft.au=Samiksha+Kaul&rft.au=Kim+N+Le&rft.au=Mei+Zhan&rft.date=2020-03-11&rft.pub=Public+Library+of+Science+%28PLoS%29&rft.eissn=1932-6203&rft.volume=15&rft.issue=3&rft.spage=e0230241&rft_id=info:doi/10.1371%2Fjournal.pone.0230241&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_a907dc2e7ba044599f10d781e5579d47 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon |