Ouabain affects cell migration via Na,K-ATPase-p130cas and via nucleus-centrosome association

• Published in: PloS one Vol. 12; no. 8; p. e0183343
• Main Authors: Ou, Young, Pan, Chen Xuan, Zuo, Jeremy, van der Hoorn, Frans A
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 17.08.2017; Public Library of Science (PLoS)
• Subjects: Amino Acid Sequence; Binding sites; Biochemistry; Biology and Life Sciences; Cell adhesion & migration; Cell growth; Cell Line; Cell migration; Cell Movement - drug effects; Cell Nucleus - drug effects; Centrosome; Centrosome - drug effects; Crk-Associated Substrate Protein - chemistry; Crk-Associated Substrate Protein - drug effects; Crk-Associated Substrate Protein - metabolism; Dephosphorylation; Dosage and administration; Enzymes; Extracellular signal-regulated kinase; Humans; Inhibitors; Kinases; Medicine; Medicine and Health Sciences; Membrane proteins; Molecular biology; Monoclonal antibodies; Na+/K+-exchanging ATPase; Nuclei; Nuclei (cytology); Ouabain; Ouabain - pharmacology; Phosphorylation; Potassium; Proteins; Research and Analysis Methods; Rodents; Signal transduction; Signaling; siRNA; Sodium; Sodium-Potassium-Exchanging ATPase - drug effects; Sodium-Potassium-Exchanging ATPase - metabolism; Src protein; Steroid hormones; Steroids; Canada; Calgary Alberta Canada

Na,K-ATPase is a membrane protein that catalyzes ATP to maintain transmembrane sodium and potassium gradients. In addition, Na,K-ATPase also acts as a signal-transducing receptor for cardiotonic steroids such as ouabain and activates a number of signalling pathways. Several studies report that ouaba...