Analysis of mitochondrial function and localisation during human embryonic stem cell differentiation in vitro

• Published in: PloS one Vol. 7; no. 12; p. e52214
• Main Authors: Prowse, Andrew B J, Chong, Fenny, Elliott, David A, Elefanty, Andrew G, Stanley, Edouard G, Gray, Peter P, Munro, Trent P, Osborne, Geoffrey W
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.12.2012; Public Library of Science (PLoS)
• Subjects: Ataxia; Bioengineering; Biology; Biosynthesis; Cardiomyocytes; Cell differentiation; Cell Differentiation - physiology; Cell Line; Contraction; Deoxyribonucleic acid; Differentiation (biology); DNA; Embryonic stem cells; Embryonic Stem Cells - cytology; Embryonic Stem Cells - metabolism; Energy requirements; Flow Cytometry; Fluorescence; Fluorescent Antibody Technique; Gene expression; Genomes; Genotype & phenotype; Green fluorescent protein; Humans; Immunology; Karyotype; Kinases; Laboratories; Localization; Mesendoderm; Metabolism; Mitochondria; Mitochondria - metabolism; Mitochondrial DNA; Nanotechnology; Oxidative Phosphorylation; Phosphorylation; Progeny; Proteins; Stem cell transplantation; Stem cells; Tissues; Australia

Human embryonic stem cell (hESC) derivatives show promise as viable cell therapy options for multiple disorders in different tissues. Recent advances in stem cell biology have lead to the reliable production and detailed molecular characterisation of a range of cell-types. However, the role of mitoc...