A Western Diet Ecological Module Identified from the ‘Humanized’ Mouse Microbiota Predicts Diet in Adults and Formula Feeding in Children

• Published in: PloS one Vol. 8; no. 12; p. e83689
• Main Authors: Siddharth, Jay, Holway, Nicholas, Parkinson, Scott J.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 31.12.2013; Public Library of Science (PLoS)
• Subjects: Abundance; Adult; Adults; Analysis; Animal models; Animals; Bacteria - classification; Bacteria - genetics; Bacteria - growth & development; Biology; Biomedical research; Bottle Feeding; Breast Feeding; Breastfeeding & lactation; Child, Preschool; Children; Children & youth; Chronic diseases; Chronic illnesses; Correlation; Datasets; Developing countries; Diet; Dietary intake; Disease; Ecological monitoring; Ecology; Fatty acids; Feces - microbiology; Feeding; Feeding Behavior; Female; Forecasts and trends; Gastrointestinal Tract - microbiology; Geographical distribution; Habits; Human populations; Humans; Infant; Infant, Newborn; Intestinal microflora; Male; Mathematical models; Medicine; Metabolism; Metagenome; Mice; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Molecular modelling; Nutrition research; Obesity; RNA, Ribosomal, 16S - genetics; Studies; Vitamins
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0083689

The interplay between diet and the microbiota has been implicated in the growing frequency of chronic diseases associated with the Western lifestyle. However, the complexity and variability of microbial ecology in humans and preclinical models has hampered identification of the molecular mechanisms underlying the association of the microbiota in this context. We sought to address two key questions. Can the microbial ecology of preclinical models predict human populations? And can we identify underlying principles that surpass the plasticity of microbial ecology in humans? To do this, we focused our study on diet; perhaps the most influential factor determining the composition of the gut microbiota. Beginning with a study in 'humanized' mice we identified an interactive module of 9 genera allied with Western diet intake. This module was applied to a controlled dietary study in humans. The abundance of the Western ecological module correctly predicted the dietary intake of 19/21 top and 21/21 of the bottom quartile samples inclusive of all 5 Western and 'low-fat' diet subjects, respectively. In 98 volunteers the abundance of the Western module correlated appropriately with dietary intake of saturated fatty acids, fat-soluble vitamins and fiber. Furthermore, it correlated with the geographical location and dietary habits of healthy adults from the Western, developing and third world. The module was also coupled to dietary intake in children (and piglets) correlating with formula (vs breast) feeding and associated with a precipitous development of the ecological module in young children. Our study provides a conceptual platform to translate microbial ecology from preclinical models to humans and identifies an ecological network module underlying the association of the gut microbiota with Western dietary habits.