Partial validation of a TaqMan quantitative polymerase chain reaction for the detection of the three genotypes of Infectious spleen and kidney necrosis virus

• Published in: PloS one Vol. 18; no. 2; p. e0281292
• Main Authors: Koda, Samantha A., Subramaniam, Kuttichantran, Hick, Paul M., Hall, Evelyn, Waltzek, Thomas B., Becker, Joy A.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 03.02.2023; Public Library of Science (PLoS)
• Subjects: Analysis; Animal diseases; Animal health; Animals; Aquaculture; Aquaculture industry; Assaying; Biology and Life Sciences; Cloning; Confidence limits; Correlation coefficient; Correlation coefficients; Deoxyribonucleic acid; Dilution; Disease; DNA; DNA Virus Infections - diagnosis; DNA Virus Infections - epidemiology; DNA Virus Infections - veterinary; DNA viruses; Earth Sciences; Epidemics; Evaluation; Fish; Fish diseases; Fish Diseases - epidemiology; Fishes; Food industry; Freshwater fish; Genetic aspects; Genomes; Genotype; Genotype & phenotype; Genotypes; Health aspects; Infections; International economic relations; Iridoviridae - genetics; Kidneys; Laboratories; Management; Marine fish; Mathematical analysis; Medical diagnosis; Medicine and Health Sciences; Mortality; Necrosis; Ornamental fish; Pathogens; Perciformes - genetics; Plasmids; Polymerase Chain Reaction; Real-Time Polymerase Chain Reaction; Research and Analysis Methods; Sea Bream - genetics; Sensitivity analysis; Spleen; Stranding; Surveillance; Viruses; United States; Australia; Red Sea

Megalocytiviruses (MCVs) are double-stranded DNA viruses known to infect important freshwater and marine fish species in the aquaculture, food, and ornamental fish industries worldwide. Infectious spleen and kidney necrosis virus (ISKNV) is the type species within the genus Megalocytivirus that caus...