Immunomodulatory Protein from Ganoderma microsporum Induces Pro-Death Autophagy through Akt-mTOR-p70S6K Pathway Inhibition in Multidrug Resistant Lung Cancer Cells

• Published in: PloS one Vol. 10; no. 5; p. e0125774
• Main Authors: Chiu, Ling-Yen, Hu, Ming-E, Yang, Tsung-Ying, Hsin, I-Lun, Ko, Jiunn-Liang, Tsai, Kan-Jen, Sheu, Gwo-Tarng
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 06.05.2015; Public Library of Science (PLoS)
• Subjects: AKT protein; Amino Acid Chloromethyl Ketones - pharmacology; Animals; Antineoplastic Agents - therapeutic use; Apoptosis; Apoptosis - drug effects; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism; Autophagy; Autophagy - drug effects; Autophagy-Related Protein 5; Basidiomycota; Calcium; Calcium (intracellular); Cancer; Cancer therapies; Carcinoma, Non-Small-Cell Lung - drug therapy; Caspase; Cell death; Cell Line, Tumor; Chemoresistance; Chemotherapy; Complications and side effects; Cytotoxicity; Drug resistance; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Drug therapy; Endoplasmic reticulum; Fungal Proteins - therapeutic use; Ganoderma; Genetic aspects; Glycoproteins; Health aspects; Homeostasis; Humans; Immunomodulation; Immunomodulators; Inhibition; Intracellular; Kinases; Lung cancer; Lung diseases; Lung Neoplasms - drug therapy; Male; Medicine; Medicine, Chinese Traditional; Mice; Mice, Inbred NOD; Microtubule-Associated Proteins - genetics; Mortality; Multidrug resistance; Multidrug resistant organisms; Non-small cell lung cancer; Non-small cell lung carcinoma; Organelles; P-Glycoprotein; Phagocytosis; Phosphorylation; Physiological aspects; Properties; Proteins; Proto-Oncogene Proteins c-akt - antagonists & inhibitors; Ribosomal Protein S6 Kinases, 70-kDa - antagonists & inhibitors; RNA Interference; RNA, Small Interfering; Rodents; Stem cells; Taxoids - therapeutic use; Thapsigargin; Thapsigargin - therapeutic use; TOR protein; TOR Serine-Threonine Kinases - antagonists & inhibitors; Toxicity; Tumor necrosis factor-TNF; Tumors; Tunicamycin; Tunicamycin - therapeutic use; Vincristine; Vincristine - therapeutic use; Xenograft Model Antitumor Assays; Xenografts; Taiwan

Chemoresistance in cancer therapy is an unfavorable prognostic factor in non-small cell lung cancer (NSCLC). Elevation of intracellular calcium level in multidrug resistant (MDR) sublines leads to sensitization of MDR sublines to cell death. We demonstrated that a fungal protein from Ganoderma micro...