In Vitro Characterization of the Impact of Different Substrates on Metabolite Production, Energy Extraction and Composition of Gut Microbiota from Lean and Obese Subjects

• Published in: PloS one Vol. 9; no. 11; p. e113864
• Main Authors: Aguirre, Marisol, Jonkers, Daisy M. A. E., Troost, Freddy J., Roeselers, Guus, Venema, Koen
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.11.2014; Public Library of Science (PLoS)
• Subjects: Abundance; Ammonia; Apples; Bacteria; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; Biology; Biology and Life Sciences; Carbohydrates; Chain branching; Chains; Chemical properties; Colon; Colon - microbiology; Dehydrogenases; Diabetes; Diet; Energy harvesting; Fatty acids; Fatty Acids, Volatile; Female; Fermentation; Food; Galactose - metabolism; Gastrointestinal Microbiome; Gastrointestinal surgery; Health promotion; Humans; Inoculum; Intestinal microflora; Lactic acid; Lactose; Lactulose; Lactulose - metabolism; Male; Metabolism; Metabolites; Microbiota; Microbiota (Symbiotic organisms); Nutrition research; Obesity; Obesity - microbiology; Oligosaccharides; Oligosaccharides - metabolism; Pectin; Phylogeny; Physiology; Prebiotics; Studies; Substrates; Sugar; Sugar beet; Sugar beets; Weight control; Netherlands

The aim of this study was to investigate the effect of galacto-oligosaccharides, lactulose, apple fiber and sugar beet pectin on the composition and activity of human colonic microbiota of lean and obese healthy subjects using an in vitro model of the proximal colon: TIM-2. Substrate fermentation wa...