Giemsa-stained wet mount based method for reticulocyte quantification: a viable alternative in resource limited or malaria endemic settings

• Published in: PloS one Vol. 8; no. 4; p. e60303
• Main Authors: Lee, Wenn-Chyau, Russell, Bruce, Lau, Yee-Ling, Fong, Mun-Yik, Chu, Cindy, Sriprawat, Kanlaya, Suwanarusk, Rossarin, Nosten, Francois, Renia, Laurent
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 02.04.2013; Public Library of Science (PLoS)
• Subjects: Analysis; Automation; Azure Stains; Biology; Blood; Cytometry; Diagnosis; Erythrocytes; Flow cytometry; Hematology; Humans; Immunology; Infections; Laboratories; Malaria; Malaria - blood; Malaria - diagnosis; Measurement; Medical examination; Medicine; Methods; Methylene blue; Methylene Blue - analogs & derivatives; Microbiology; Microscopy; Parasitology; Plasmodium vivax; Reticulocyte Count - methods; Reticulocytes; Reticulocytes - parasitology; Stains; Stains & staining; Vector-borne diseases; United States; Kuala Lumpur Malaysia; Malaysia; Singapore; Thailand

The quantity of circulating reticulocytes is an important indicator of erythropoietic activity in response to a wide range of haematological pathologies. While most modern laboratories use flow cytometry to quantify reticulocytes, most field laboratories still rely on 'subvital' staining....