DNA Vaccine Delivered by a Needle-Free Injection Device Improves Potency of Priming for Antibody and CD8+ T-Cell Responses after rAd5 Boost in a Randomized Clinical Trial

• Published in: PloS one Vol. 8; no. 4; p. e59340
• Main Authors: Graham, Barney S., Enama, Mary E., Nason, Martha C., Gordon, Ingelise J., Peel, Sheila A., Ledgerwood, Julie E., Plummer, Sarah A., Mascola, John R., Bailer, Robert T., Roederer, Mario, Koup, Richard A., Nabel, Gary J.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 08.04.2013; Public Library of Science (PLoS)
• Subjects: Adenoviridae - genetics; Adenoviruses; Adolescent; Adult; Adults; Antibodies, Viral - immunology; Avian flu; Binding sites; Biology; Carbon dioxide; CD4 antigen; CD8 antigen; CD8-Positive T-Lymphocytes - immunology; Clinical trials; Delivery scheduling; Deoxyribonucleic acid; DNA; DNA vaccines; DNA, Recombinant - genetics; Dose-Response Relationship, Immunologic; Drug delivery systems; Enzyme-linked immunosorbent assay; Female; Hepatitis; HIV; HIV-1 - immunology; HIV-1 - metabolism; Human immunodeficiency virus; Humans; Immunity, Cellular - immunology; Immunity, Humoral - immunology; Immunization, Secondary - methods; Immunogenicity; Infectious diseases; Injection; Injections; Interferon; Lymphocytes T; Male; Medicine; Middle Aged; Peptide Fragments - metabolism; Priming; Safety; Safety regulations; Studies; Syringes; T cells; Vaccination; Vaccination - instrumentation; Vaccines; Vaccines, DNA - administration & dosage; Vaccines, DNA - adverse effects; Vaccines, DNA - genetics; Vaccines, DNA - immunology; Vice presidents (Organizations); West Nile virus; Young Adult; γ-Interferon; United States > US; Maryland

DNA vaccine immunogenicity has been limited by inefficient delivery. Needle-free delivery of DNA using a CO2-powered Biojector® device was compared to delivery by needle and syringe and evaluated for safety and immunogenicity. Forty adults, 18-50 years, were randomly assigned to intramuscular (IM) v...