Proteomic Biomarkers for Ageing the Mosquito Aedes aegypti to Determine Risk of Pathogen Transmission

• Published in: PloS one Vol. 8; no. 3; p. e58656
• Main Authors: Hugo, Leon E., Monkman, James, Dave, Keyur A., Wockner, Leesa F., Birrell, Geoff W., Norris, Emma L., Kienzle, Vivian J., Sikulu, Maggy T., Ryan, Peter A., Gorman, Jeffery J., Kay, Brian H.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 11.03.2013; Public Library of Science (PLoS)
• Subjects: Abundance; Actin; Aedes - metabolism; Aedes aegypti; Age; Aging; Animals; Aquatic insects; Biological markers; Biology; Biomarkers; Biomarkers - metabolism; Culicidae; Dengue; Dengue fever; Dengue virus; Disease transmission; Drosophila melanogaster; Electron transfer; Epidemiology; Fat body; Female; Fever; Gel electrophoresis; Gene expression; Genetic vectors; Health risks; Homeostasis; Initiation factor eIF-5A; Insect control; Insect Vectors - metabolism; Insects; Laboratories; Malaria; Manganese; Mass spectrometry; Mass spectroscopy; Medical research; Medicine; Mosquitoes; Multivariate analysis; Muscle proteins; Oxidoreductase; Pathogens; Polypeptides; Post-translation; Protein expression; Proteins; Proteome; Proteomics; Senescence; Superoxide dismutase; Vector-borne diseases; Viral diseases; Viruses; Queensland Australia; Australia
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0058656

Biomarkers of the age of mosquitoes are required to determine the risk of transmission of various pathogens as each pathogen undergoes a period of extrinsic incubation in the mosquito host. Using the 2-D Difference Gel Electrophoresis (2-D DIGE) procedure, we investigated the abundance of up to 898...