Molecular characterization of multidrug resistant hospital isolates using the antimicrobial resistance determinant microarray
Molecular methods that enable the detection of antimicrobial resistance determinants are critical surveillance tools that are necessary to aid in curbing the spread of antibiotic resistance. In this study, we describe the use of the Antimicrobial Resistance Determinant Microarray (ARDM) that targets...
Saved in:
| Published in | PloS one Vol. 8; no. 7; p. e69507 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Public Library of Science
25.07.2013
Public Library of Science (PLoS) |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Molecular methods that enable the detection of antimicrobial resistance determinants are critical surveillance tools that are necessary to aid in curbing the spread of antibiotic resistance. In this study, we describe the use of the Antimicrobial Resistance Determinant Microarray (ARDM) that targets 239 unique genes that confer resistance to 12 classes of antimicrobial compounds, quaternary amines and streptothricin for the determination of multidrug resistance (MDR) gene profiles. Fourteen reference MDR strains, which either were genome, sequenced or possessed well characterized drug resistance profiles were used to optimize detection algorithms and threshold criteria to ensure the microarray's effectiveness for unbiased characterization of antimicrobial resistance determinants in MDR strains. The subsequent testing of Acinetobacter baumannii, Escherichia coli and Klebsiella pneumoniae hospital isolates revealed the presence of several antibiotic resistance genes [e.g. belonging to TEM, SHV, OXA and CTX-M classes (and OXA and CTX-M subfamilies) of β-lactamases] and their assemblages which were confirmed by PCR and DNA sequence analysis. When combined with results from the reference strains, ~25% of the ARDM content was confirmed as effective for representing allelic content from both Gram-positive and -negative species. Taken together, the ARDM identified MDR assemblages containing six to 18 unique resistance genes in each strain tested, demonstrating its utility as a powerful tool for molecular epidemiological investigations of antimicrobial resistance in clinically relevant bacterial pathogens. |
|---|---|
| AbstractList | Molecular methods that enable the detection of antimicrobial resistance determinants are critical surveillance tools that are necessary to aid in curbing the spread of antibiotic resistance. In this study, we describe the use of the Antimicrobial Resistance Determinant Microarray (ARDM) that targets 239 unique genes that confer resistance to 12 classes of antimicrobial compounds, quaternary amines and streptothricin for the determination of multidrug resistance (MDR) gene profiles. Fourteen reference MDR strains, which either were genome, sequenced or possessed well characterized drug resistance profiles were used to optimize detection algorithms and threshold criteria to ensure the microarray's effectiveness for unbiased characterization of antimicrobial resistance determinants in MDR strains. The subsequent testing of Acinetobacter baumannii, Escherichia coli and Klebsiella pneumoniae hospital isolates revealed the presence of several antibiotic resistance genes [e.g. belonging to TEM, SHV, OXA and CTX-M classes (and OXA and CTX-M subfamilies) of β-lactamases] and their assemblages which were confirmed by PCR and DNA sequence analysis. When combined with results from the reference strains, ∼25% of the ARDM content was confirmed as effective for representing allelic content from both Gram-positive and –negative species. Taken together, the ARDM identified MDR assemblages containing six to 18 unique resistance genes in each strain tested, demonstrating its utility as a powerful tool for molecular epidemiological investigations of antimicrobial resistance in clinically relevant bacterial pathogens. Molecular methods that enable the detection of antimicrobial resistance determinants are critical surveillance tools that are necessary to aid in curbing the spread of antibiotic resistance. In this study, we describe the use of the Antimicrobial Resistance Determinant Microarray (ARDM) that targets 239 unique genes that confer resistance to 12 classes of antimicrobial compounds, quaternary amines and streptothricin for the determination of multidrug resistance (MDR) gene profiles. Fourteen reference MDR strains, which either were genome, sequenced or possessed well characterized drug resistance profiles were used to optimize detection algorithms and threshold criteria to ensure the microarray's effectiveness for unbiased characterization of antimicrobial resistance determinants in MDR strains. The subsequent testing of Acinetobacter baumannii, Escherichia coli and Klebsiella pneumoniae hospital isolates revealed the presence of several antibiotic resistance genes [e.g. belonging to TEM, SHV, OXA and CTX-M classes (and OXA and CTX-M subfamilies) of β-lactamases] and their assemblages which were confirmed by PCR and DNA sequence analysis. When combined with results from the reference strains, ~25% of the ARDM content was confirmed as effective for representing allelic content from both Gram-positive and -negative species. Taken together, the ARDM identified MDR assemblages containing six to 18 unique resistance genes in each strain tested, demonstrating its utility as a powerful tool for molecular epidemiological investigations of antimicrobial resistance in clinically relevant bacterial pathogens. Molecular methods that enable the detection of antimicrobial resistance determinants are critical surveillance tools that are necessary to aid in curbing the spread of antibiotic resistance. In this study, we describe the use of the Antimicrobial Resistance Determinant Microarray (ARDM) that targets 239 unique genes that confer resistance to 12 classes of antimicrobial compounds, quaternary amines and streptothricin for the determination of multidrug resistance (MDR) gene profiles. Fourteen reference MDR strains, which either were genome, sequenced or possessed well characterized drug resistance profiles were used to optimize detection algorithms and threshold criteria to ensure the microarray's effectiveness for unbiased characterization of antimicrobial resistance determinants in MDR strains. The subsequent testing of Acinetobacter baumannii , Escherichia coli and Klebsiella pneumoniae hospital isolates revealed the presence of several antibiotic resistance genes [e.g. belonging to TEM, SHV, OXA and CTX-M classes (and OXA and CTX-M subfamilies) of β-lactamases] and their assemblages which were confirmed by PCR and DNA sequence analysis. When combined with results from the reference strains, ∼25% of the ARDM content was confirmed as effective for representing allelic content from both Gram-positive and –negative species. Taken together, the ARDM identified MDR assemblages containing six to 18 unique resistance genes in each strain tested, demonstrating its utility as a powerful tool for molecular epidemiological investigations of antimicrobial resistance in clinically relevant bacterial pathogens. Molecular methods that enable the detection of antimicrobial resistance determinants are critical surveillance tools that are necessary to aid in curbing the spread of antibiotic resistance. In this study, we describe the use of the Antimicrobial Resistance Determinant Microarray (ARDM) that targets 239 unique genes that confer resistance to 12 classes of antimicrobial compounds, quaternary amines and streptothricin for the determination of multidrug resistance (MDR) gene profiles. Fourteen reference MDR strains, which either were genome, sequenced or possessed well characterized drug resistance profiles were used to optimize detection algorithms and threshold criteria to ensure the microarray's effectiveness for unbiased characterization of antimicrobial resistance determinants in MDR strains. The subsequent testing of Acinetobacter baumannii, Escherichia coli and Klebsiella pneumoniae hospital isolates revealed the presence of several antibiotic resistance genes [e.g. belonging to TEM, SHV, OXA and CTX-M classes (and OXA and CTX-M subfamilies) of [beta]-lactamases] and their assemblages which were confirmed by PCR and DNA sequence analysis. When combined with results from the reference strains, ~25% of the ARDM content was confirmed as effective for representing allelic content from both Gram-positive and -negative species. Taken together, the ARDM identified MDR assemblages containing six to 18 unique resistance genes in each strain tested, demonstrating its utility as a powerful tool for molecular epidemiological investigations of antimicrobial resistance in clinically relevant bacterial pathogens. |
| Audience | Academic |
| Author | Pimentel, Guillermo Nicklasson, Matilda House, Brent L Barrows, Brian R Taitt, Chris Rowe Wasfy, Momtaz Abdel-Maksoud, Mohamed Leski, Tomasz A Vora, Gary J |
| AuthorAffiliation | 1 Center for Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, D.C., United States of America 2 National Research Council Postdoctoral Associate at US Naval Research Laboratory, Washington, D.C., United States of America University of Malaya, Malaysia 3 US Naval Medical Research Unit No. 3, Cairo, Egypt |
| AuthorAffiliation_xml | – name: University of Malaya, Malaysia – name: 3 US Naval Medical Research Unit No. 3, Cairo, Egypt – name: 1 Center for Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, D.C., United States of America – name: 2 National Research Council Postdoctoral Associate at US Naval Research Laboratory, Washington, D.C., United States of America |
| Author_xml | – sequence: 1 givenname: Tomasz A surname: Leski fullname: Leski, Tomasz A organization: Center for Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, DC, United States of America – sequence: 2 givenname: Gary J surname: Vora fullname: Vora, Gary J – sequence: 3 givenname: Brian R surname: Barrows fullname: Barrows, Brian R – sequence: 4 givenname: Guillermo surname: Pimentel fullname: Pimentel, Guillermo – sequence: 5 givenname: Brent L surname: House fullname: House, Brent L – sequence: 6 givenname: Matilda surname: Nicklasson fullname: Nicklasson, Matilda – sequence: 7 givenname: Momtaz surname: Wasfy fullname: Wasfy, Momtaz – sequence: 8 givenname: Mohamed surname: Abdel-Maksoud fullname: Abdel-Maksoud, Mohamed – sequence: 9 givenname: Chris Rowe surname: Taitt fullname: Taitt, Chris Rowe |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23936031$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNk1uL1DAUx4usuBf9BqIFQfRhxqRp0_RFWBYvAysL3l7DaXoykyHTjEkqruB3NzPTWWZkH6QPDSe__z_JuZxnJ73rMcueUjKlrKZvlm7wPdjpOoWnhPCmIvWD7Iw2rJjwgrCTg_Vpdh7CkpCKCc4fZacFaxgnjJ5lfz45i2qw4HO1AA8qoje_IRrX507nq8FG0_lhnnsMJkToY75wYW0i2NwEZyFiyIdg-nkeF5infbMyyrvWJGCvUZh3mIxXpt8YbAHwHm4fZw812IBPxv9F9u39u69XHyfXNx9mV5fXE8WbIk4KBK5LTkWhW1EJLpoOy0LxUhOhEdta61YpWrQcGBZM6ZpA2WlCGihJxQW7yJ7vfNfWBTlmLkhaFrWgTFQ0EbMd0TlYyrU3K_C30oGR24Dzcwk-GmVRll1dEoWFproqeVO36XJI20owaEVaJK-342lDu8JOYR892CPT453eLOTc_ZSsToWhVTJ4NRp492PAEOXKBIXWQo9u2N6bUE4aXib0xT_o_a8bqTmkB5heu3Su2pjKy7IWoqGEbrym91Dp6zCVLLWZNil-JHh9JEhMxF9xDkMIcvbl8_-zN9-P2ZcH7ALBxkXqtWHTleEYLHdgaqgQPOq7JFMiN1Oyz4bcTIkcpyTJnh0W6E60Hwv2FzUPEss |
| CitedBy_id | crossref_primary_10_1016_j_ab_2019_113405 crossref_primary_10_1038_s41598_017_18972_w crossref_primary_10_1038_srep27930 crossref_primary_10_1128_msphere_01020_21 crossref_primary_10_1371_journal_pone_0231829 crossref_primary_10_1038_s41598_017_18506_4 crossref_primary_10_3389_frabi_2023_1126468 crossref_primary_10_1016_j_csbj_2022_12_041 crossref_primary_10_1016_j_jgar_2015_05_006 crossref_primary_10_1128_AAC_01897_13 crossref_primary_10_1371_journal_pone_0178880 crossref_primary_10_1016_j_meegid_2017_09_019 crossref_primary_10_3390_ijms21165928 crossref_primary_10_3390_ijms21051669 crossref_primary_10_1016_j_micpath_2023_106066 |
| Cites_doi | 10.1248/bpb.28.1505 10.1089/mdr.2010.0063 10.1111/j.1348-0421.2011.00323.x 10.1128/JB.06749-11 10.1093/jac/dkq095 10.1093/jac/dkp256 10.1002/elan.200603603 10.1016/j.aca.2010.09.007 10.1128/JCM.06115-11 10.1093/jac/dkp453 10.1093/jac/dkp431 10.1128/JB.184.6.1712-1721.2002 10.1128/AAC.46.6.2054-2055.2002 10.1128/JCM.43.9.4862-4864.2005 10.1093/jac/dkp255 10.3855/jidc.386 10.1128/JCM.43.5.2291-2302.2005 10.1016/j.ijantimicag.2004.01.010 10.1016/j.ajic.2011.04.009 10.1093/jac/dki074 10.1016/j.ijantimicag.2007.11.017 10.1186/1471-2458-11-S2-S8 10.1016/j.actatropica.2009.04.004 10.4315/0362-028X-72.3.624 10.1128/JB.187.7.2426-2438.2005 10.1111/j.1348-0421.2009.00168.x 10.1093/jac/dkg101 10.1016/S0140-6736(00)04403-2 10.1186/1471-2334-9-84 10.1128/JCM.01943-07 10.1111/j.1574-6976.2011.00273.x 10.1128/JCM.02607-10 10.1111/j.1348-0421.2012.00429.x 10.1128/JCM.01771-09 10.1016/j.ijantimicag.2006.01.007 10.1089/mdr.2009.0082 10.1186/1476-0711-7-12 10.1089/mdr.2006.9998 10.1038/nm1347 10.1016/j.ijantimicag.2012.08.015 10.1016/j.ijantimicag.2012.05.019 10.1126/science.1080613 10.1016/S0924-8579(07)70870-1 10.1128/AAC.39.1.185 10.1186/1756-0500-2-76 10.1101/gad.1510307 10.1089/mdr.2012.0008 10.3109/00365540903493715 10.1128/AAC.44.9.2382-2388.2000 10.1093/jac/dkq049 10.1292/jvms.09-0538 10.1089/mdr.1995.1.195 10.1128/AAC.01574-05 10.1128/JCM.41.8.3542-3547.2003 10.1016/j.vetmic.2008.11.021 10.1371/journal.pgen.0020007 10.1159/000321242 10.1016/j.mib.2010.09.006 10.1111/j.1863-2378.2010.01358.x |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2013 Public Library of Science 2013. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2013 |
| Copyright_xml | – notice: COPYRIGHT 2013 Public Library of Science – notice: 2013. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2013 |
| DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PIMPY PQEST PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 5PM DOA |
| DOI | 10.1371/journal.pone.0069507 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Gale In Context: Opposing Viewpoints Gale In Context: Science ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest Central UK/Ireland Advanced Technologies & Aerospace Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection ProQuest Databases Technology Collection ProQuest Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Materials Science Collection ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection Biological Sciences Agriculture Science Database Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection Publicly Available Content Database ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Biological Science Collection ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic Technology Collection Technology Research Database Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | Agricultural Science Database MEDLINE |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) Medicine Biology |
| DocumentTitleAlternate | Antimicrobial Resistance Determinant Microarray |
| EISSN | 1932-6203 |
| Editor | Sekaran, Shamala Devi |
| Editor_xml | – sequence: 1 givenname: Shamala Devi surname: Sekaran fullname: Sekaran, Shamala Devi |
| EndPage | e69507 |
| ExternalDocumentID | 1427813851 oai_doaj_org_article_4d740ce2f1f54697ba6fe1b583ab8e1b 3054328551 A478891014 10_1371_journal_pone_0069507 23936031 |
| Genre | Research Support, U.S. Gov't, Non-P.H.S Journal Article |
| GeographicLocations | Egypt Washington DC United States--US Maryland |
| GeographicLocations_xml | – name: Egypt – name: Maryland – name: United States--US – name: Washington DC |
| GroupedDBID | --- 123 29O 2WC 3V. 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ ABDBF ABIVO ABJCF ABUWG ACGFO ACIHN ACIWK ACPRK ADBBV ADRAZ AEAQA AENEX AFKRA AFPKN AFRAH AHMBA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BBORY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CGR CS3 CUY CVF D1I D1J D1K DIK DU5 E3Z EAP EAS EBD ECM EIF EMOBN ESTFP ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IHR IHW INH INR IOV IPNFZ IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ NPM O5R O5S OK1 P2P P62 PATMY PDBOC PIMPY PQQKQ PROAC PSQYO PTHSS PV9 PYCSY RIG RNS RPM RZL SV3 TR2 UKHRP WOQ WOW ~02 ~KM AAYXX CITATION 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO FR3 GNUQQ H94 K9. KL. M7N P64 PQEST PQUKI PRINS RC3 7X8 5PM - 02 AAPBV ABPTK ADACO BBAFP KM |
| ID | FETCH-LOGICAL-c692t-2ea6f46182fb858689de42c64f08feeb7ffbcc12b6a3e23cf70a4df009a405683 |
| IEDL.DBID | RPM |
| ISSN | 1932-6203 |
| IngestDate | Fri Nov 26 17:14:11 EST 2021 Fri Jul 05 11:48:56 EDT 2024 Tue Sep 17 21:26:17 EDT 2024 Wed Jul 24 14:57:37 EDT 2024 Fri Sep 13 08:08:33 EDT 2024 Thu Feb 22 23:41:04 EST 2024 Fri Feb 02 04:04:38 EST 2024 Thu Aug 01 19:46:05 EDT 2024 Thu Aug 01 19:33:54 EDT 2024 Tue Aug 20 22:14:26 EDT 2024 Fri Aug 23 02:25:07 EDT 2024 Sat Sep 28 07:55:15 EDT 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 7 |
| Language | English |
| License | This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Creative Commons CC0 public domain |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c692t-2ea6f46182fb858689de42c64f08feeb7ffbcc12b6a3e23cf70a4df009a405683 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist. Current address: US Naval Medical Research Center-Frederick, Fort Detrick, Maryland, United States of America Current address: Agricultural Research Service, US Department of Agriculture, Beltsville, Maryland, United States of America Current address: University of Gothenburg, Göteborg, Sweden Conceived and designed the experiments: TAL GJV CRT GP. Performed the experiments: TAL GJV CRT BRB BLH MN MW MAM. Analyzed the data: TAL GJV CRT GP. Contributed reagents/materials/analysis tools: TAL GJV CRT BRB BLH MN MW MAM. Wrote the paper: TAL GJV CRT. Designed the software used in analysis: TAL. |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3723915/ |
| PMID | 23936031 |
| PQID | 1427813851 |
| PQPubID | 1436336 |
| PageCount | e69507 |
| ParticipantIDs | plos_journals_1427813851 doaj_primary_oai_doaj_org_article_4d740ce2f1f54697ba6fe1b583ab8e1b pubmedcentral_primary_oai_pubmedcentral_nih_gov_3723915 proquest_miscellaneous_1420160964 proquest_journals_1427813851 gale_infotracmisc_A478891014 gale_infotracacademiconefile_A478891014 gale_incontextgauss_ISR_A478891014 gale_incontextgauss_IOV_A478891014 gale_healthsolutions_A478891014 crossref_primary_10_1371_journal_pone_0069507 pubmed_primary_23936031 |
| PublicationCentury | 2000 |
| PublicationDate | 2013-07-25 |
| PublicationDateYYYYMMDD | 2013-07-25 |
| PublicationDate_xml | – month: 07 year: 2013 text: 2013-07-25 day: 25 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: San Francisco – name: San Francisco, USA |
| PublicationTitle | PloS one |
| PublicationTitleAlternate | PLoS One |
| PublicationYear | 2013 |
| Publisher | Public Library of Science Public Library of Science (PLoS) |
| Publisher_xml | – name: Public Library of Science – name: Public Library of Science (PLoS) |
| References | 16369542 - Nat Med. 2006 Jan;12(1):83-8 20920882 - Curr Opin Microbiol. 2010 Oct;13(5):558-64 23040010 - Int J Antimicrob Agents. 2013 Jan;41(1):90-1 20375036 - J Antimicrob Chemother. 2010 Jun;65(6):1162-70 12019141 - Antimicrob Agents Chemother. 2002 Jun;46(6):2054-5 11872723 - J Bacteriol. 2002 Mar;184(6):1712-21 22416992 - Microb Drug Resist. 2012 Aug;18(4):364-71 21083822 - Zoonoses Public Health. 2010 Nov;57 Suppl 1:94-9 16145160 - J Clin Microbiol. 2005 Sep;43(9):4862-4 16079502 - Biol Pharm Bull. 2005 Aug;28(8):1505-8 21454989 - Med Princ Pract. 2011;20(3):213-9 10952583 - Antimicrob Agents Chemother. 2000 Sep;44(9):2382-8 20121649 - Scand J Infect Dis. 2010 Apr;42(4):275-80 19996141 - J Antimicrob Chemother. 2010 Feb;65(2):249-51 20008447 - J Antimicrob Chemother. 2010 Feb;65(2):361-2 16723563 - Antimicrob Agents Chemother. 2006 Jun;50(6):2038-41 17536935 - Microb Drug Resist. 2007 Spring;13(1):62-8 16415984 - PLoS Genet. 2006 Jan;2(1):e7 15288309 - Int J Antimicrob Agents. 2004 Aug;24(2):119-24 22500933 - Microbiol Immunol. 2012 Apr;56(4):254-61 19675016 - J Antimicrob Chemother. 2009 Sep;64 Suppl 1:i29-36 9158775 - Microb Drug Resist. 1995 Fall;1(3):195-202 20145377 - J Vet Med Sci. 2010 Jun;72(6):727-34 18243668 - Int J Antimicrob Agents. 2008 May;31(5):440-51 19343954 - J Food Prot. 2009 Mar;72(3):624-30 21325547 - J Clin Microbiol. 2011 Apr;49(4):1608-13 21835504 - Am J Infect Control. 2011 Nov;39(9):e61-5 20200038 - J Antimicrob Chemother. 2010 May;65(5):872-5 22374953 - J Bacteriol. 2012 Mar;194(6):1619-20 12615864 - J Antimicrob Chemother. 2003 Mar;51(3):625-30 7695304 - Antimicrob Agents Chemother. 1995 Jan;39(1):185-91 20130382 - J Infect Dev Ctries. 2010 Jan;4(1):58-60 15761065 - J Antimicrob Chemother. 2005 May;55(5):768-72 22322349 - J Clin Microbiol. 2012 May;50(5):1632-9 19375408 - Acta Trop. 2009 Aug;111(2):144-9 21128836 - Microb Drug Resist. 2011 Mar;17(1):67-73 19675017 - J Antimicrob Chemother. 2009 Sep;64 Suppl 1:i3-10 19497111 - BMC Infect Dis. 2009;9:84 12663927 - Science. 2003 Mar 28;299(5615):2071-4 20181912 - J Clin Microbiol. 2010 Apr;48(4):1323-32 15774886 - J Bacteriol. 2005 Apr;187(7):2426-38 19916789 - Microb Drug Resist. 2010 Mar;16(1):9-19 16713187 - Int J Antimicrob Agents. 2006 Jun;27(6):545-8 21338385 - Microbiol Immunol. 2011 May;55(5):318-27 12904353 - J Clin Microbiol. 2003 Aug;41(8):3542-7 18513394 - Ann Clin Microbiol Antimicrob. 2008;7:12 15872258 - J Clin Microbiol. 2005 May;43(5):2291-302 19419535 - BMC Res Notes. 2009 May 06;2:76 21517914 - FEMS Microbiol Rev. 2011 Sep;35(5):790-819 19903259 - Microbiol Immunol. 2009 Nov;53(11):595-602 20577157 - MMWR Morb Mortal Wkly Rep. 2010 Jun 25;59(24):750 20951861 - Anal Chim Acta. 2010 Oct 29;679(1-2):85-90 19128900 - Vet Microbiol. 2009 May 12;136(3-4):397-402 11418146 - Lancet. 2001 Apr 21;357(9264):1225-40 22817914 - Int J Antimicrob Agents. 2012 Sep;40(3):282-4 18094139 - J Clin Microbiol. 2008 Feb;46(2):707-12 21388568 - BMC Public Health. 2011;11 Suppl 2:S8 17344419 - Genes Dev. 2007 Mar 1;21(5):601-14 ref14 AM Ahmed (ref41) 2011; 55 M Al-Agamy (ref40) 2006; 13 L Poirel (ref24) 2013; 41 AM Ahmed (ref43) 2009; 136 HW Stokes (ref12) 2011; 35 K Bush (ref1) 2010; 13 J Wojciechowski (ref16) 2010; 679 L Poirel (ref25) 2003; 41 DV Zurawski (ref13) 2012; 194 SK Bouchillon (ref59) 2004; 24 D Szabo (ref33) 2008; 7 S Abdel Ghani (ref37) 2010; 4 K Hansson (ref54) 2002; 184 M Kuroda (ref67) 2001; 357 IT Paulsen (ref69) 2003; 299 H Fang (ref17) 2008; 46 K Veldman (ref47) 2010; 65 ND Hanson (ref4) 2010; 5 N Fam (ref35) 2011; 17 Y Ishida (ref46) 2010; 72 C Levesque (ref52) 1995; 39 B Sallen (ref53) 1995; 1 A El Kholy (ref21) 2003; 51 AM Ahmed (ref45) 2009; 111 W Meyer (ref20) 2011; 11 NA Naiemi (ref31) 2005; 43 MH Mohamed Al-Agamy (ref58) 2006; 27 T Naas (ref8) 2011; 49 V Perreten (ref11) 2005; 43 PE Fournier (ref55) 2006; 2 ref30 K Ojo (ref50) 2002; 46 MG Smith (ref61) 2007; 21 ref32 M Cassone (ref7) 2006; 50 AM Hammad (ref44) 2009; 72 G Kikuvi (ref49) 1983; 13 JK Rasheed (ref63) 2000; 44 T Leski (ref19) 2012; 40 AM Ahmed (ref42) 2012; 56 S Anantham (ref48) 2012; 18 NG Khalaf (ref28) 2009; 9 M Salem (ref57) 2010; 4 M Batchelor (ref9) 2008; 31 L Poirel (ref36) 2007; 29, Suppl 2 SH Ahmed (ref27) 2009; 2 V Post (ref56) 2010; 65 W Ogawa (ref64) 2005; 28 JG Frye (ref10) 2010; 16 F Pasteran (ref26) 2010; 48 PM Hawkey (ref2) 2009; 64 Suppl 1 P Garneau (ref6) 2010; 57 Suppl 1 M Al-Agamy (ref39) 2006; 27 DM Livermore (ref3) 2009; 64 Suppl 1 J Tham (ref60) 2010; 42 T Saied (ref23) 2011; 39 M Al-Agamy (ref29) 2012; 6 P Bogaerts (ref18) 2010; 65 G Al Hashem (ref38) 2011; 20 CG Carvalhaes (ref65) 2010; 65 A Robicsek (ref51) 2006; 12 SR Gill (ref68) 2005; 187 DM Tracz (ref62) 2005; 55 (ref66) 2010; 59 C Lascols (ref5) 2012; 50 N Fam (ref34) 2008; 3 A Hussein (ref22) 2009; 53 KM Roth (ref15) 2006; 18 |
| References_xml | – volume: 28 start-page: 1505 year: 2005 ident: ref64 article-title: Multidrug resistance in Klebsiella pneumoniae MGH78578 and cloning of genes responsible for the resistance publication-title: Biol Pharm Bull doi: 10.1248/bpb.28.1505 contributor: fullname: W Ogawa – volume: 17 start-page: 67 year: 2011 ident: ref35 article-title: CTX-M-15-producing Escherichia coli clinical isolates in Cairo (Egypt), including isolates of clonal complex ST10 and clones ST131, ST73, and ST405 in both community and hospital settings publication-title: Microb Drug Resist doi: 10.1089/mdr.2010.0063 contributor: fullname: N Fam – volume: 55 start-page: 318 year: 2011 ident: ref41 article-title: Molecular characterization of antimicrobial resistance in Gram-negative bacteria isolated from bovine mastitis in Egypt publication-title: Microbiol Immunol doi: 10.1111/j.1348-0421.2011.00323.x contributor: fullname: AM Ahmed – volume: 4 start-page: 398 year: 2010 ident: ref57 article-title: Distribution of classes 1 and 2 integrons among multi drug resistant E. coli isolated from hospitalized patients with urinary tract infection in Cairo, Egypt publication-title: Aust J Basic Appl Sci contributor: fullname: M Salem – volume: 194 start-page: 1619 year: 2012 ident: ref13 article-title: Genome sequences of four divergent multidrug-resistant Acinetobacter baumannii strains isolated from patients with sepsis or osteomyelitis publication-title: J Bacteriol doi: 10.1128/JB.06749-11 contributor: fullname: DV Zurawski – volume: 65 start-page: 1162 year: 2010 ident: ref56 article-title: Evolution of AbaR-type genomic resistance islands in multiply antibiotic-resistant Acinetobacter baumannii publication-title: J Antimicrob Chemother doi: 10.1093/jac/dkq095 contributor: fullname: V Post – volume: 64 Suppl 1 start-page: i3 year: 2009 ident: ref2 article-title: The changing epidemiology of resistance publication-title: J Antimicrob Chemother doi: 10.1093/jac/dkp256 contributor: fullname: PM Hawkey – volume: 18 start-page: 1982 year: 2006 ident: ref15 article-title: Electrochemical detection of short DNA oligomer hybridization using the CombiMatrix ElectraSense microarray reader publication-title: Electroanalysis doi: 10.1002/elan.200603603 contributor: fullname: KM Roth – volume: 679 start-page: 85 year: 2010 ident: ref16 article-title: Enhancement of deoxyribonucleic acid microarray performance using post-hybridization signal amplification publication-title: Anal Chim Acta doi: 10.1016/j.aca.2010.09.007 contributor: fullname: J Wojciechowski – volume: 6 start-page: 20 year: 2012 ident: ref29 article-title: Genetic basis of cefotaxime resistant isolates of Klebsiella pneumoniae from Cairo publication-title: African J Microbiol Res contributor: fullname: M Al-Agamy – volume: 50 start-page: 1632 year: 2012 ident: ref5 article-title: Using nucleic acid microarrays to perform molecular epidemiology and detect novel β-lactamases: A snapshot of ESBLs throughout the world publication-title: J Clin Microbiol doi: 10.1128/JCM.06115-11 contributor: fullname: C Lascols – volume: 65 start-page: 361 year: 2010 ident: ref18 article-title: Emergence of clonally related Klebsiella pneumoniae isolates of sequence type 258 producing KPC-2 carbapenemase in Belgium publication-title: J Antimicrob Chemother doi: 10.1093/jac/dkp453 contributor: fullname: P Bogaerts – volume: 65 start-page: 249 year: 2010 ident: ref65 article-title: Cloverleaf test (modified Hodge test) for detecting carbapenemase production in Klebsiella pneumoniae: be aware of false positive results publication-title: J Antimicrob Chemother doi: 10.1093/jac/dkp431 contributor: fullname: CG Carvalhaes – volume: 184 start-page: 1712 year: 2002 ident: ref54 article-title: IntI2 integron integrase in Tn7 publication-title: J Bacteriol doi: 10.1128/JB.184.6.1712-1721.2002 contributor: fullname: K Hansson – volume: 46 start-page: 2054 year: 2002 ident: ref50 article-title: Identification of a complete dfrA14 gene cassette integrated at a secondary site in a resistance plasmid of uropathogenic Escherichia coli from Nigeria publication-title: Antimicrob Agents Chemother doi: 10.1128/AAC.46.6.2054-2055.2002 contributor: fullname: K Ojo – volume: 3 start-page: 84 year: 2008 ident: ref34 article-title: CTX-M-15 extended-spectrum β-lactamases detected from intensive care unit of an Egyptian medical research institute publication-title: Res J Med Med Sci contributor: fullname: N Fam – volume: 43 start-page: 4862 year: 2005 ident: ref31 article-title: Widespread transfer of resistance genes between bacterial species in an intensive care unit: implications for hospital epidemiology publication-title: J Clin Microbiol doi: 10.1128/JCM.43.9.4862-4864.2005 contributor: fullname: NA Naiemi – ident: ref30 – volume: 64 Suppl 1 start-page: i29 year: 2009 ident: ref3 article-title: Has the era of untreatable infections arrived? publication-title: J Antimicrob Chemother doi: 10.1093/jac/dkp255 contributor: fullname: DM Livermore – volume: 4 start-page: 058 year: 2010 ident: ref37 article-title: First report of CTX-M-14-producing clinical isolates of Salmonella serovar Typhimurium from Egypt publication-title: J Infect Dev Ctries doi: 10.3855/jidc.386 contributor: fullname: S Abdel Ghani – volume: 43 start-page: 2291 year: 2005 ident: ref11 article-title: Microarray-based detection of 90 antibiotic resistance genes of Gram-positive bacteria publication-title: J Clin Microbiol doi: 10.1128/JCM.43.5.2291-2302.2005 contributor: fullname: V Perreten – volume: 24 start-page: 119 year: 2004 ident: ref59 article-title: Determining incidence of extended spectrum beta-lactamase producing Enterobacteriaceae, vancomycin-resistant Enterococcus faecium and methicillin-resistant Staphylococcus aureus in 38 centres from 17 countries: The PEARLS study 2001–2002 publication-title: Int J Antimicrob Agents doi: 10.1016/j.ijantimicag.2004.01.010 contributor: fullname: SK Bouchillon – volume: 39 start-page: e61 year: 2011 ident: ref23 article-title: Antimicrobial resistance in pathogens causing nosocomial bloodstream infections in university hospitals in Egypt publication-title: Am J Infect Control doi: 10.1016/j.ajic.2011.04.009 contributor: fullname: T Saied – volume: 55 start-page: 768 year: 2005 ident: ref62 article-title: Increase in ampC promoter strength due to mutations and deletion of the attenuator in a clinical isolate of cefoxitin-resistant Escherichia coli as determined by RT-PCR publication-title: J Antimicrob Chemother doi: 10.1093/jac/dki074 contributor: fullname: DM Tracz – volume: 31 start-page: 440 year: 2008 ident: ref9 article-title: Development of a miniaturised microarray-based assay for the rapid identification of antimicrobial resistance genes in Gram-negative bacteria publication-title: Int J Antimicrob Agents doi: 10.1016/j.ijantimicag.2007.11.017 contributor: fullname: M Batchelor – volume: 13 start-page: 17 year: 2006 ident: ref40 article-title: Detection of chloramphenicol resistance genes in broad spectrum β-lactamase-producing Escherichia coli strains publication-title: N Egypt J Microbiol contributor: fullname: M Al-Agamy – volume: 11 start-page: S8 year: 2011 ident: ref20 article-title: Antimicrobial resistance surveillance in the AFHSC-GEIS network publication-title: BMC Public Health doi: 10.1186/1471-2458-11-S2-S8 contributor: fullname: W Meyer – volume: 111 start-page: 144 year: 2009 ident: ref45 article-title: Genetic basis of multidrug resistance in Salmonella enterica serovars Enteritidis and Typhimurium isolated from diarrheic calves in Egypt publication-title: Acta Tropica doi: 10.1016/j.actatropica.2009.04.004 contributor: fullname: AM Ahmed – volume: 72 start-page: 624 year: 2009 ident: ref44 article-title: Prevalence and molecular characterization of ampicillin-resistant Enterobacteriaceae isolated from traditional Egyptian Domiati cheese publication-title: J Food Protect doi: 10.4315/0362-028X-72.3.624 contributor: fullname: AM Hammad – volume: 187 start-page: 2426 year: 2005 ident: ref68 article-title: Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain publication-title: J Bacteriol doi: 10.1128/JB.187.7.2426-2438.2005 contributor: fullname: SR Gill – volume: 53 start-page: 595 year: 2009 ident: ref22 article-title: Characterization of integrons and antimicrobial resistance genes in clinical isolates of Gram-negative bacteria from Palestinian hospitals publication-title: Microbiol Immunol doi: 10.1111/j.1348-0421.2009.00168.x contributor: fullname: A Hussein – volume: 51 start-page: 625 year: 2003 ident: ref21 article-title: Antimicrobial resistance in Cairo, Egypt 1999–2000: A survey of five hospitals publication-title: J Antimicrob Chemother doi: 10.1093/jac/dkg101 contributor: fullname: A El Kholy – volume: 357 start-page: 1225 year: 2001 ident: ref67 article-title: Whole genome sequencing of methicillin-resistant Staphylococcus aureus publication-title: Lancet doi: 10.1016/S0140-6736(00)04403-2 contributor: fullname: M Kuroda – volume: 5 start-page: 333 year: 2010 ident: ref4 article-title: Molecular diagnostics could help in coping with hidden β-lactamases publication-title: Microbe contributor: fullname: ND Hanson – volume: 9 start-page: 84 year: 2009 ident: ref28 article-title: Characterization of CTX-M ESBLs in Enterobacter cloacae, Escherichia coli and Klebsiella pneumoniae clinical isolates from Cairo, Egypt publication-title: BMC Infect Dis doi: 10.1186/1471-2334-9-84 contributor: fullname: NG Khalaf – volume: 46 start-page: 707 year: 2008 ident: ref17 article-title: Molecular epidemiology of extended-spectrum β-lactamases among Escherichia coli isolates collected in a Swedish hospital and its associated health care facilities from 2001 to 2006 publication-title: J Clin Microbiol doi: 10.1128/JCM.01943-07 contributor: fullname: H Fang – volume: 35 start-page: 790 year: 2011 ident: ref12 article-title: Gene flow, mobile genetic elements and the recruitment of antibiotic resistance genes into Gram-negative pathogens publication-title: FEMS Microbiol Rev doi: 10.1111/j.1574-6976.2011.00273.x contributor: fullname: HW Stokes – volume: 49 start-page: 1608 year: 2011 ident: ref8 article-title: Evaluation of a DNA microarray (Check-MDR CT102) for rapid detection of TEM, SHV, and CTX-M extended-spectrum β-lactamases and of KPC, OXA-48, VIM, IMP, and NDM-1 carbapenemases publication-title: J Clin Microbiol doi: 10.1128/JCM.02607-10 contributor: fullname: T Naas – volume: 56 start-page: 254 year: 2012 ident: ref42 article-title: Genetic analysis of multiple antimicrobial resistance in Salmonella isolated from diseased broilers in Egypt publication-title: Microbiol Immunol doi: 10.1111/j.1348-0421.2012.00429.x contributor: fullname: AM Ahmed – volume: 48 start-page: 1323 year: 2010 ident: ref26 article-title: Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of Enterobacteriaceae by incorporating boronic acid publication-title: J Clin Microbiol doi: 10.1128/JCM.01771-09 contributor: fullname: F Pasteran – volume: 27 start-page: 545 year: 2006 ident: ref39 article-title: First description of CTX-M β-lactamase-producing clinical Escherichia coli isolates from Egypt publication-title: Int J Antimicrob Agents doi: 10.1016/j.ijantimicag.2006.01.007 contributor: fullname: M Al-Agamy – volume: 16 start-page: 9 year: 2010 ident: ref10 article-title: Development of a DNA microarray to detect antimicrobial resistance genes identified in the National Center for Biotechnology Information database publication-title: Microb Drug Resist doi: 10.1089/mdr.2009.0082 contributor: fullname: JG Frye – volume: 7 start-page: 12 year: 2008 ident: ref33 article-title: Imported PER-1 producing Pseudomonas aeruginosa, PER-1 producing Acinetobacter baumannii and VIM-2-producing Pseudomonas aeruginosa strains in Hungary publication-title: Ann Clin Microbiol Antimicrob doi: 10.1186/1476-0711-7-12 contributor: fullname: D Szabo – volume: 13 start-page: 62 year: 1983 ident: ref49 article-title: Streptomycin and chloramphenicol resistance genes in Escherichia coli isolates from cattle, pigs, and chicken in Kenya publication-title: Microb Drug Resist doi: 10.1089/mdr.2006.9998 contributor: fullname: G Kikuvi – volume: 27 start-page: 545 year: 2006 ident: ref58 article-title: First description of CTX-M β-lactamase-producing clinical Escherichia coli isolates from Egypt publication-title: Int J Antimicrob Agents doi: 10.1016/j.ijantimicag.2006.01.007 contributor: fullname: MH Mohamed Al-Agamy – ident: ref32 – volume: 12 start-page: 83 year: 2006 ident: ref51 article-title: Fluoroquinolone-modifying enzyme: A new adaptation of a common aminoglyocide acetyltransferase publication-title: Nat Med doi: 10.1038/nm1347 contributor: fullname: A Robicsek – volume: 41 start-page: 90 year: 2013 ident: ref24 article-title: Occurrence of OXA-48 and VIM-1 carbapenemase-producing Enterobacteriaceae in Egypt publication-title: Int J Antimicrob Agents doi: 10.1016/j.ijantimicag.2012.08.015 contributor: fullname: L Poirel – volume: 40 start-page: 282 year: 2012 ident: ref19 article-title: Multidrug resistance determinants from NDM-1 producing Klebsiella pneumoniae, United States of America publication-title: Int J Antimicrob Agents doi: 10.1016/j.ijantimicag.2012.05.019 contributor: fullname: T Leski – volume: 299 start-page: 2071 year: 2003 ident: ref69 article-title: Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis publication-title: Science doi: 10.1126/science.1080613 contributor: fullname: IT Paulsen – volume: 29, Suppl 2 start-page: S276 year: 2007 ident: ref36 article-title: P1029 Explosive emergence of CTX-M-15 extended-spectrum β-lactamase in Enterobacteriaceae in Kuwait publication-title: Int J Antimicrob Agents doi: 10.1016/S0924-8579(07)70870-1 contributor: fullname: L Poirel – volume: 39 start-page: 185 year: 1995 ident: ref52 article-title: PCR mapping of integrons reveals several novel combinations of resistance genes publication-title: Antimicrob Agents Chemother doi: 10.1128/AAC.39.1.185 contributor: fullname: C Levesque – volume: 2 start-page: 76 year: 2009 ident: ref27 article-title: Nosocomial blood stream infection in intensive care units at Assiut University Hospitals (Upper Egypt) with special reference to extended spectrum β-lactamase producing organisms publication-title: BMC Res Notes doi: 10.1186/1756-0500-2-76 contributor: fullname: SH Ahmed – volume: 21 start-page: 601 year: 2007 ident: ref61 article-title: New insights into Acinetobacter baumannii pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis publication-title: Genes Dev doi: 10.1101/gad.1510307 contributor: fullname: MG Smith – volume: 18 start-page: 364 year: 2012 ident: ref48 article-title: pCERC1, a small, globally disseminated plasmid carrying the dfrA14 cassette in the strA gene of the sul2-strA-strB gene cluster publication-title: Microb Drug Resist doi: 10.1089/mdr.2012.0008 contributor: fullname: S Anantham – volume: 42 start-page: 275 year: 2010 ident: ref60 article-title: Extended-spectrum β-lactamase-producing Escherichia coli in patients with travellers' diarrhoea publication-title: Scand J Infect Dis doi: 10.3109/00365540903493715 contributor: fullname: J Tham – volume: 44 start-page: 2382 year: 2000 ident: ref63 article-title: Characterization of the extended-spectrum β-lactamase reference strain, Klebsiella pneumoniae K6 (ATCC 700603), which produces the novel enzyme SHV-18 publication-title: Antimicrob Agents Chemother doi: 10.1128/AAC.44.9.2382-2388.2000 contributor: fullname: JK Rasheed – volume: 65 start-page: 872 year: 2010 ident: ref47 article-title: Characterizatin of multidrug-resistant, qnrB2-positive and extended-spectrum-β-lactamase-producing Salmonella Concord and Salmonella Senftenberg isolates publication-title: J Antimicrob Chemother doi: 10.1093/jac/dkq049 contributor: fullname: K Veldman – volume: 72 start-page: 727 year: 2010 ident: ref46 article-title: Molecular analysis of antimicrobial resistance in Gram-negative bacteria isolated from fish farms in Egypt publication-title: J Vet Med Sci doi: 10.1292/jvms.09-0538 contributor: fullname: Y Ishida – volume: 1 start-page: 195 year: 1995 ident: ref53 article-title: Molecular epidemiology of integron-associated antibiotic-resistance genes in clinical isolates of Enterobacteriaceae publication-title: Microb Drug Resist doi: 10.1089/mdr.1995.1.195 contributor: fullname: B Sallen – volume: 50 start-page: 2038 year: 2006 ident: ref7 article-title: DNA microarray for detection of macrolide resistance genes publication-title: Antimicrob Agents Chemother doi: 10.1128/AAC.01574-05 contributor: fullname: M Cassone – volume: 41 start-page: 3542 year: 2003 ident: ref25 article-title: Outbreak of extended-spectrum β-lactamase VEB-1-producing isolates of Acinetobacter baumannii in a French hospital publication-title: J Clin Microbiol doi: 10.1128/JCM.41.8.3542-3547.2003 contributor: fullname: L Poirel – volume: 136 start-page: 397 year: 2009 ident: ref43 article-title: Genetic analysis of antimicrobial resistance in Escherichia coli isolated from diarrheic neonatal calves publication-title: Vet Microbiol doi: 10.1016/j.vetmic.2008.11.021 contributor: fullname: AM Ahmed – volume: 59 start-page: 750 year: 2010 ident: ref66 article-title: Detection of Enterobacteriaceae isolates carrying metallo-β-lactamase - United States, 2010 publication-title: MMWR Morb Mortal Wkly Rep – volume: 2 start-page: e7 year: 2006 ident: ref55 article-title: Comparative genomics of multidrug resistance in Acinetobacter baumannii publication-title: PLoS Genet doi: 10.1371/journal.pgen.0020007 contributor: fullname: PE Fournier – volume: 20 start-page: 213 year: 2011 ident: ref38 article-title: Sequence analysis of blaCTX-M genes carried by clinically significant Escherichia coli isolates in Kuwait hospitals publication-title: Med Princip Pract doi: 10.1159/000321242 contributor: fullname: G Al Hashem – volume: 13 start-page: 558 year: 2010 ident: ref1 article-title: Alarming β-lactamase-mediated resistance in multidrug-resistant Enterobacteriaceae publication-title: Curr Opin Microbiol doi: 10.1016/j.mib.2010.09.006 contributor: fullname: K Bush – volume: 57 Suppl 1 start-page: 94 year: 2010 ident: ref6 article-title: Use of a bacterial antimicrobial resistance gene microarray for the identification of resistant Staphylococcus aureus publication-title: Zoonoses Public Health doi: 10.1111/j.1863-2378.2010.01358.x contributor: fullname: P Garneau – ident: ref14 |
| SSID | ssj0053866 |
| Score | 2.253669 |
| Snippet | Molecular methods that enable the detection of antimicrobial resistance determinants are critical surveillance tools that are necessary to aid in curbing the... |
| SourceID | plos doaj pubmedcentral proquest gale crossref pubmed |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database |
| StartPage | e69507 |
| SubjectTerms | Acinetobacter baumannii Acinetobacter baumannii - drug effects Acinetobacter baumannii - enzymology Acinetobacter baumannii - genetics Acinetobacter baumannii - isolation & purification Acinetobacter Infections - drug therapy Acinetobacter Infections - epidemiology Acinetobacter Infections - microbiology Algorithms Amines Analysis Anti-Bacterial Agents - pharmacology Antibiotic resistance Antibiotics Antiinfectives and antibacterials Antimicrobial agents Antimicrobial resistance Bacteria beta-Lactamases - genetics beta-Lactamases - metabolism Biology Deoxyribonucleic acid Determinants DNA DNA microarrays DNA sequencing DNA, Bacterial - analysis DNA, Bacterial - genetics Drug resistance Drug Resistance, Multiple, Bacterial - genetics E coli Egypt - epidemiology Enterobacteriaceae Enterococcus faecalis Epidemiology Escherichia coli Escherichia coli - drug effects Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli Infections - drug therapy Escherichia coli Infections - epidemiology Escherichia coli Infections - microbiology Evolution Genes Genomes Genomics Humans Klebsiella Klebsiella Infections - drug therapy Klebsiella Infections - epidemiology Klebsiella Infections - microbiology Klebsiella pneumoniae Klebsiella pneumoniae - drug effects Klebsiella pneumoniae - enzymology Klebsiella pneumoniae - genetics Klebsiella pneumoniae - isolation & purification Laboratories Medical research Medicine Microbial drug resistance Multidrug resistance Multidrug resistant organisms Nucleotide sequence Oligonucleotide Array Sequence Analysis - methods Polymerase Chain Reaction Quaternary Sequence Analysis, DNA Staphylococcus aureus Staphylococcus epidermidis Staphylococcus infections Strains (organisms) Streptothricin Urogenital system |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELbQnrggyquBFgxCAg6hieO1nWNBVAWpIAFFvUW2Y29XgmS12T30wH9nxnHCBlWCA7doPYmy87C_UWa-IeS5KJGXSulUl1akcB7L1GjD0tJmrgB8KkqP_c5nH8XpOf9wMb_YGfWFNWE9PXCvuCNeS55Zx3zu55DKSaOFd7mZq0IbBRdh983nQzLV78EQxULERrlC5kfRLq9XbeOwkquc4_jYnYMo8PWPu_Js9b3troOcf1ZO7hxFJ7fJrYgh6XH_7nvkhmvukL0YpR19GamkX90lP8-G6bfUjszMfeMlbT0N1YT1erugkHQjkGw29DIOEqFL8EoEohRL4xcUgCKF9eWPZaBuAoHhHuto_buohgYBvV7rq3vk_OTd17enaRy4kFqw2SZlDpTLBaQc3iiwkyprx5kV3GfKO2ek98banBmhC8cK62Wmee0BpmnAfUIV98msARXvE8q8QiJ4kSmec8u15rLwUnPNXVkDykpIOmi_WvW8GlX4uCYhH-nVWKG1qmithLxBE42yyIodfgBfqaKvVH_zlYQ8QQNXfYvpGNvVMc4QKHFqcUKeBQlkxmiw9Gaht11Xvf_07R-EvnyeCL2IQr4FV7E6tjvAf0LGrYnkwUQS4ttOlvfRHQetdJCsManyAqAy3Dm46PXLT8dlfCiW0zWu3QYZZBYsBTz9Qe_Ro2aREw9HjydETnx9ovrpSrO8DMTkhWQ4b-Dh_7DVI3KThckjSCRyQGab9dYdAv7bmMch1H8Bw0hdBQ priority: 102 providerName: Directory of Open Access Journals – databaseName: ProQuest Databases dbid: BENPR link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3Nb9MwFLdGd-GCGF8rDDAICTiENolrJyfUjU0dUgcqDO0W2Y7dVYKkNOmBA_877zlOtqAJcYvilyh5H_ZL_N7vR8grniIuVSIDmWoewHosAiVVFKR6bGLIT3lqsd95fsZn5-zjxeRih8zaXhgsq2znRDdR56XGf-SjEDkhwhgShJFU-BdA16P3658B8kfhPqsn07hFdqOQ4Ybt7uHx2edFOytDXHPuW-diEY68pd6ty8JgbVc6QULZa0uTQ_Dv5unB-ntZ3ZSE_l1LeW1xOrlL7viskk4bN9gjO6a4R_Z83Fb0jQeXfnuf_J63fLj0qMNqbloxaWmp68fNN9slXZgKU8uipi21CD0FP8XUlLo6AwqpI50W9erHyoE5gUB7jTb0w1WZDZ2jgNxs5K8H5Pzk-OvRLPAUDIEGK9ZBZCS3jMNHiFUJWC5Jc8MizZkdJ9YYJaxVWoeR4jI2UaytGEuWW0jcJGSCPIkfkkEBKt4nNLIJQsPzccJCppmUTMRWSCaZSXPIu4YkaLWfrRukjcxttwn4QmnUmKG1Mm-tITlEE3WyiJPtTpSbZebDLmO5YGNtIhvaCeOpUPA6JlSTJJYqgYMheY4Gzpqm0y7asymyCqTIYzwkL50EYmUUWIyzlNuqyk4_ffsPoS-LntBrL2RL9F_pGyDgnRCDqyd50JOEiNe94X10x1YrVXYVG3Bl66I3D7_ohvGmWGBXmHLrZBBrMOVw90eNR3eaRZQ8JCMfEtHz9Z7q-yPF6tJBlcciQgaCx_9-rCfkduRYRhA05IAM6s3WPIVcr1bPfBj_AW0_V4U priority: 102 providerName: ProQuest – databaseName: Scholars Portal Open Access Journals dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Lb9QwELaq5cIFUV5NKWAQEnBIlTheOzkgtBSqFmlBKizqLXIce1mpTbZJVqIH_jszzgOCFqm31XpsyfOIP8sz3xDyUiTISxUrXyVa-HAeSz9TGfMTHZgI8KlILNY7zz-LkwX_dD493yF9z9ZOgfXWqx32k1pUF4c_r67fQcC_dV0bZNhPOlyXhcE8rWSK5eW3GI84-vycD-8KEN3u9RJRiy9YEHXFdP9bZXRYOU7_4cs9WV-U9TZY-m925V_H1fFdcqfDmXTWOsYu2THFPbLbRXJNX3d002_uk1_zvkMuPRrYm9viTFpa6ip082qzpGemRrBZNLRvNkJPwXMRrFKXeUABTNJZ0awuV47eCQT6OdrQD38Sb-gcBVRVqesHZHH88dvRid81ZfA12LXxmVHCcgHXEpvFYMs4yQ1nWnAbxNaYTFqbaR2yTKjIsEhbGSieW4ByCrChiKOHZFKAivcIZTZGsngRxDzkmivFZWSl4oqbJAck5hG_1366brk3UvcAJ-HO0qoxRWulnbU88h5NNMgic7b7o6yWaReIKc8lD7RhNrRTLhKZwXZMmE3jSGUx_PDIMzRw2pahDvGfzrDPQIKdjT3ywkkge0aB6TlLtanr9PTL9xsIfT0bCb3qhGwJrqJVVxIBe0JWrpHkwUgSvgF6NLyH7thrpYYLHZNxGAGchpm9i24ffj4M46KYcleYcuNkkH0wEbD6o9ajB80ibx62J_eIHPn6SPXjkWL1w5GXR5JhT4L9G2_9MbnNXAsSZBQ5IJOm2pgnAASb7KmL7d_rm15A priority: 102 providerName: Scholars Portal |
| Title | Molecular characterization of multidrug resistant hospital isolates using the antimicrobial resistance determinant microarray |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/23936031 https://www.proquest.com/docview/1427813851/abstract/ https://search.proquest.com/docview/1420160964 https://pubmed.ncbi.nlm.nih.gov/PMC3723915 https://doaj.org/article/4d740ce2f1f54697ba6fe1b583ab8e1b http://dx.doi.org/10.1371/journal.pone.0069507 |
| Volume | 8 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj9MwELZ2i0BcEFseW1iKQUjAIW0Tu3Zy7JaWXaQuq8Ki3iLHtUulbVL1cdgD_HZmnAcE7QFxsaJ4HDWeGftzM_MNIW9EhLxUofJUpIUH-7H0EpUEXqR7hgE-FZHFfOfJhTi74p9m_dkB6Ze5MC5oXyfLTnq96qTL7y62cr3S3TJOrHs5GTIZIK9595AcSsbKI3q-_IIDC1HkyDHpdwuVdNZZajCIKwIAhAzALGJYYLm2HTnW_mptbqyvs-1twPPv-Mk_NqTxQ_KgQJJ0kP_iI3Jg0ia5m9eWvGmSe5Piq3mTHBUOvKXvCpbp94_Ij0lZGJcOK9LmPCeTZpa6xNz5Zr-gU7NFjJnuaFljhJ6DwSJGpS7ggAKGpIN0t1wtHasTCJRjtKEffsfb0AkKqM1G3TwmV-PR1-GZV9Ri8DSoc-cFRgnLBZxGbBKCCsNobnigBbe90BqTSGsTrf0gEYqZgGkre4rPLSA4BZBQhOwJaaSggmNCAxsiR7zohdznmivFJbNSccVNNAcA1iJeqZJ4nVNuxO67m4SjSj63MWozLrTZIqeot0oWCbPdjWyziAuziflc8p42gfVtn4tIJvA6xk_6IVNJCBct8hK1HufZp5XbxwMsLxBhQeMWee0kkDQjxaichdpvt_H552__IPRlWhN6WwjZDOxHqyITAt4Jybhqkic1SXB9Xes-RhstZ2UL57hAhj4DFA0jS7u9vftV1Y0PxUi71GR7J4Okg5GApz_Nzbya2dJpWkTWHKA29fUecGHHWV647LP_Hvmc3A9cJRIkFjkhjd1mb14AHtwlbVgFZhLacOhjO_7YJndORxeX07b7hwXaCQ-x_Tlqu7XiFzVlaLU |
| link.rule.ids | 230,315,733,786,790,870,891,2115,2236,12083,12250,12792,21416,24346,27957,27958,31754,31755,33301,33302,33408,33409,33779,33780,43345,43614,43635,43840,53827,53829,74102,74371,74392,74659 |
| linkProvider | National Library of Medicine |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3Nb9MwFLegHOCCGF8rDGYQEnAIy4drJydUClML65DGhnazHMculSApTXvgwP_Oe46TLWhC3KL4JUreh_2cvPf7EfKCZ4hLlapAZZoHsB6LIFd5HGQ6NAnkpzyz2O88P-bTM_bxfHTuP7jVvqyynRPdRF1UGr-RH0TICRElkCC8Xf0MkDUK_656Co3r5AZLYKHBTvFJV-IBscy5b5dLRHTgrfNmVZUG67myEZLIXlqOHGp_NzcPVt-r-qrE8-_6yUsL0uEdcttnknTcmH6HXDPlXbLjY7Wmrzyg9Ot75Pe85cClkw6fuWm_pJWlrge3WG8X9MTUmE6WG9rSidAZ-Camo9TVFlBIF-m43Cx_LB2AEwi012hD31-U1tA5Cqj1Wv26T84OP5xOpoGnXQg0WG4TxEZxyzhsPGyegrXSrDAs1pzZMLXG5MLaXOsozrlKTJxoK0LFCgvJmoLsj6fJAzIoQcW7hMY2RTh4HqYsYpopxURihWKKmayAXGtIglb7ctWga0j3i03ArqRRo0RrSW-tIXmHJupkERvbnajWC-lDTbJCsFCb2EZ2BJt_kcPrmCgfpYnKUzgYkn00sGwaTbsIl2NkEsiQu3hInjsJxMcosQBnobZ1LWefv_6H0JeTntBLL2QrcBWtfNMDvBPibvUk93qSEOW6N7yL7thqpZYX8QBXti569fCzbhhvikV1pam2TgbxBTMOd3_YeHSnWUTGQwLyIRE9X--pvj9SLr85ePJExMg68Ojfj7VPbk5P50fyaHb86TG5FTuWEQQN2SODzXprnkCut8mfuoD-A7_OVR0 |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1bb9MwFLagSIgXxLitMJhBSMBDaC6unTyhsjGtQAcaDPXNchy7VIKkNO0DD_x3znGcbEET4q2qT6LmXOzPzfH3EfKMZ8hLlapAZZoHsB6LIFd5HGQ6NAngU55ZPO88O-HHZ-zdfDz3_U-1b6ts50Q3UReVxv_IRxFqQkQJAISR9W0Rnw6PXq9-BqgghW9avZzGVXINVskQ1QzEvNt8QV1z7o_OJSIa-Ui9WlWlwd6ubIyCsheWJsfg383Tg9X3qr4MhP7dS3lhcTq6RW56VEknTRrskCumvE12fN3W9IUnl355h_yetXq49KDjam6OYtLKUncet1hvF_TU1Agtyw1tpUXoFPIUoSl1fQYUoCOdlJvlj6UjcwKD9hpt6OF5mw2doYFar9Wvu-Ts6O2Xg-PASzAEGqK4CWKjuGUcNiE2TyFyaVYYFmvObJhaY3Jhba51FOdcJSZOtBWhYoUF4KYACfI0uUcGJbh4l9DYpkgNz8OURUwzpZhIrFBMMZMVgLuGJGi9L1cN04Z0r9sE7FAaN0qMlvTRGpI3GKLOFnmy3RfVeiF92UlWCBZqE9vIjhnPRA6PY6J8nCYqT-HDkOxjgGVz6LSrdjlBVYEMdYyH5KmzQK6MErNuobZ1Lacfv_6H0efTntFzb2QrSBWt_AEIeCbk4OpZ7vUsoeJ1b3gX07H1Si3PawOubFP08uEn3TDeFBvsSlNtnQ1yDWYc7n6_yejOs8iSh2LkQyJ6ud5zfX-kXH5zVOWJiFGB4MG_f9Y-uQ61LD9MT94_JDdiJziC_CF7ZLBZb80jgH2b_LGr5z8O_Vl- |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Molecular+Characterization+of+Multidrug+Resistant+Hospital+Isolates+Using+the+Antimicrobial+Resistance+Determinant+Microarray&rft.jtitle=PloS+one&rft.au=Leski%2C+Tomasz+A&rft.au=Vora%2C+Gary+J&rft.au=Barrows%2C+Brian+R&rft.au=Pimentel%2C+Guillermo&rft.date=2013-07-25&rft.pub=Public+Library+of+Science&rft.issn=1932-6203&rft.eissn=1932-6203&rft.volume=8&rft.issue=7&rft.spage=e69507&rft_id=info:doi/10.1371%2Fjournal.pone.0069507&rft.externalDocID=A478891014 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon |