A Comparison between Droplet Digital and Quantitative PCR in the Analysis of Bacterial 16S Load in Lung Tissue Samples from Control and COPD GOLD 2

• Published in: PloS one Vol. 9; no. 10; p. e110351
• Main Authors: Sze, Marc A., Abbasi, Meysam, Hogg, James C., Sin, Don D.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.10.2014; Public Library of Science (PLoS)
• Subjects: Airway management; Analysis; Assaying; Background noise; Bacteria; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - pathogenicity; Biology and Life Sciences; Chronic obstructive lung disease; Chronic obstructive pulmonary disease; Coefficient of variation; Control methods; Correlation analysis; Cystic fibrosis; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; E coli; Efficiency; Escherichia coli; Ethics; Gold; Histology; Humans; Laboratories; Lung - microbiology; Lung - pathology; Lung diseases; Medicine; Medicine and Health Sciences; Microbiomes; Microbiota - genetics; New technology; Noise reduction; Obstructive lung disease; Pathology; Polymerase Chain Reaction; Primers; Pulmonary Disease, Chronic Obstructive - genetics; Pulmonary Disease, Chronic Obstructive - microbiology; Pulmonary Disease, Chronic Obstructive - pathology; Research and Analysis Methods; RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Surgery; Tissues; Canada

Low biomass in the bacterial lung tissue microbiome utilizes quantitative PCR (qPCR) 16S bacterial assays at their limit of detection. New technology like droplet digital PCR (ddPCR) could allow for higher sensitivity and accuracy of quantification. These attributes are needed if specific bacteria w...