State-of-the-art analytical methods of viral infections in human lung organoids

• Published in: PloS one Vol. 17; no. 12; p. e0276115
• Main Authors: Baumgardt, Morris, Hülsemann, Maren, Löwa, Anna, Fatykhova, Diana, Hoffmann, Karen, Kessler, Mirjana, Mieth, Maren, Hellwig, Katharina, Frey, Doris, Langenhagen, Alina, Voss, Anne, Obermayer, Benedikt, Wyler, Emanuel, Dökel, Simon, Gruber, Achim D., Tölch, Ulf, Hippenstiel, Stefan, Hocke, Andreas C., Hönzke, Katja
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 20.12.2022; Public Library of Science (PLoS)
• Subjects: ACE2; Alveoli; Analysis; Analytical methods; Angiotensin; Angiotensin-converting enzyme 2; Angiotensin-Converting Enzyme 2 - metabolism; Biology and life sciences; Cell culture; Cell cycle; Control; Coronaviruses; COVID-19; COVID-19 - metabolism; Diagnosis; E protein; Epidemics; Flow cytometry; Furin; Furin - metabolism; Gene expression; Gene sequencing; Germany; Human performance; Humans; Hybridization; Immunohistochemistry; Impact analysis; Infections; Lab Protocol; Lung - metabolism; Medical research; Medicine and health sciences; mRNA; Organoids; Pandemics; Peptidyl-dipeptidase A; Plaque assay; Proteins; Replication; Research and Analysis Methods; Ribonucleic acid; RNA; RNA processing; SARS-CoV-2; Serine proteinase; Severe acute respiratory syndrome coronavirus 2; Tropism; Viral diseases; Viral infections; Virulence; Virulence (Microbiology); Virus diseases; Viruses; Germany

Human-based organ models can provide strong predictive value to investigate the tropism, virulence, and replication kinetics of viral pathogens. Currently, such models have received widespread attention in the study of SARS-CoV-2 causing the COVID-19 pandemic. Applicable to a large set of organoid m...