Constructing a human complex type N-linked glycosylation pathway in Kluyveromyces marxianus

• Published in: PloS one Vol. 15; no. 5; p. e0233492
• Main Authors: Lee, Ming-Hsuan, Hsu, Tsui-Ling, Lin, Jinn-Jy, Lin, Yu-Ju, Kao, Yi-Ying, Chang, Jui-Jen, Li, Wen-Hsiung
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29.05.2020; Public Library of Science (PLoS)
• Subjects: Biodiversity; Biological activity; Biological properties; Biology and Life Sciences; Biotechnology; Composition; CRISPR; CRISPR-Cas systems; Deoxyribonucleic acid; DNA; Genes; Genetic aspects; Genetic engineering; Genomics; Glycan; Glycoproteins; Glycosylation; Growth rate; Homologous recombination; Homology; Immunogenicity; Kluyveromyces marxianus; Mannose; Mannosidase; Methods; Microbial genetic engineering; Microorganisms; N-Acetylglucosamine; N-Acetylglucosinyldiphosphodolichol N-acetylglucosaminyltransferase; Physical Sciences; Probiotics; Protein engineering; Proteins; Research and Analysis Methods; Yeast; Yeasts; Yeasts (Fungi); Taiwan; China

Glycosylation can affect various protein properties such as stability, biological activity, and immunogenicity. To produce human therapeutic proteins, a host that can produce glycoproteins with correct glycan structures is required. Microbial expression systems offer economical, rapid and serum-free...