Increased threshold of short-latency motor evoked potentials in transgenic mice expressing Channelrhodopsin-2

• Published in: PloS one Vol. 12; no. 5; p. e0178803
• Main Authors: Wu, Wei, Xiong, Wenhui, Zhang, Ping, Chen, Lifang, Fang, Jianqiao, Shields, Christopher, Xu, Xiao-Ming, Jin, Xiaoming
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 31.05.2017; Public Library of Science (PLoS)
• Subjects: Action potential; Acupuncture; Amplitudes; Animals; Assessments; Biology; Biology and Life Sciences; Brain research; Brain stem; Channelrhodopsins; Cortex (motor); Current injection; Electric Stimulation; Electrical stimuli; Electrodes; Evoked potentials; Evoked Potentials, Motor; Excitability; Firing; Genes; Genetic aspects; Genetic engineering; Genetically modified mice; Health aspects; Injection; Laboratory animals; Latency; Magnetic fields; Medicine; Medicine and Health Sciences; Mice; Mice, Inbred C57BL; Mice, Transgenic; Morphology; Motor evoked potentials; Motor task performance; Nervous system; Neural networks; Neurons; Neurosciences; Patch-Clamp Techniques; Pyramidal cells; Pyramidal tracts; Research and Analysis Methods; Reticulospinal tract; Reticulospinal tracts; Rhodopsin; Rodents; Surgery; Transcranial magnetic stimulation; Transcription; Transgenic animals; Transgenic mice; Traumatic brain injury; Walking; Indiana; United States > US; Indianapolis Indiana; Kentucky

Transgenic mice that express channelrhodopsin-2 or its variants provide a powerful tool for optogenetic study of the nervous system. Previous studies have established that introducing such exogenous genes usually does not alter anatomical, electrophysiological, and behavioral properties of neurons i...