Identification of host genes involved in geminivirus infection using a reverse genetics approach

• Published in: PloS one Vol. 6; no. 7; p. e22383
• Main Authors: Lozano-Durán, Rosa, Rosas-Díaz, Tábata, Luna, Ana P, Bejarano, Eduardo R
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.07.2011; Public Library of Science (PLoS)
• Subjects: Analysis; Antiviral agents; Apium graveolens; Biology; Cloning, Molecular; Enzymes; Feasibility studies; Geminiviridae - physiology; Gene expression; Gene Silencing; Genes; Genes, Plant - genetics; Genetic Association Studies; Genetic engineering; Genetic research; Genetically modified plants; Genetics; Genomes; Green Fluorescent Proteins - metabolism; Health aspects; Host plants; Host-Pathogen Interactions - genetics; Infection; Infections; Kinases; Leaves; Machinery and equipment; Medical research; Molecular modelling; Nicotiana - genetics; Nicotiana - virology; Phenotype; Plant diseases; Plant Diseases - genetics; Plant Diseases - virology; Plant viruses; Plants, Genetically Modified; Proteins; Research parks; Reverse Genetics - methods; Studies; Tobacco; Tomatoes; Transgenic plants; Viral infections; Viruses; Yellow leaf

Geminiviruses, like all viruses, rely on the host cell machinery to establish a successful infection, but the identity and function of these required host proteins remain largely unknown. Tomato yellow leaf curl Sardinia virus (TYLCSV), a monopartite geminivirus, is one of the causal agents of the d...