Transgene Excision Has No Impact on In Vivo Integration of Human iPS Derived Neural Precursors

• Published in: PloS one Vol. 6; no. 9; p. e24687
• Main Authors: Major, Tamara, Menon, Jayanthi, Auyeung, Gordon, Soldner, Frank, Hockemeyer, Dirk, Jaenisch, Rudolf, Tabar, Viviane
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.09.2011; Public Library of Science (PLoS)
• Subjects: Animals; Astrocytes; Astrocytes - cytology; Astrocytes - metabolism; B cells; Biology; C cells; c-Myc protein; Cancer; Cell Differentiation; Cell Movement; Cells, Cultured; Cloning; Corpus Striatum - surgery; Cues; Differentiation; Dopamine; Embryos; Epigenetics; Female; Fibroblasts; Gene expression; Glial Fibrillary Acidic Protein - analysis; Grafting; Humans; Immunohistochemistry; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Lymphocytes B; Medicine; Microtubule-Associated Proteins - analysis; Myc protein; Neostriatum; Neural stem cells; Neural Stem Cells - cytology; Neural Stem Cells - metabolism; Neurons; Neurons - cytology; Neurons - metabolism; Neuropeptides - analysis; Neurosciences; Neurosurgery; Olfactory bulb; Oligodendrocytes; Oligodendroglia - cytology; Oligodendroglia - metabolism; Pluripotency; Precursors; Progeny; Rats; Rats, Sprague-Dawley; Rodents; Stem cell transplantation; Stem Cell Transplantation - methods; Stem cells; Studies; Subventricular zone; Teratoma; Transcription factors; Transgenes; Transgenes - genetics; Transplantation, Heterologous; Tubulin - analysis; Viral genetics; New York; United States > US; Massachusetts

The derivation of induced human pluripotent stem cells (hiPS) has generated significant enthusiasm particularly for the prospects of cell-based therapy. But there are concerns about the suitability of iPS cells for in vivo applications due in part to the introduction of potentially oncogenic transcr...