PI 3 kinase related kinases-independent proteolysis of BRCA1 regulates Rad51 recruitment during genotoxic stress in human cells

• Published in: PloS one Vol. 5; no. 11; p. e14027
• Main Authors: Hammond-Martel, Ian, Pak, Helen, Yu, Helen, Rouget, Raphael, Horwitz, Andrew A, Parvin, Jeffrey D, Drobetsky, Elliot A, Affar, El Bachir
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 17.11.2010; Public Library of Science (PLoS)
• Subjects: Adducts; Alkylation; Analysis; Antineoplastic Agents, Alkylating - pharmacology; Apoptosis; Apoptosis - drug effects; Apoptosis - radiation effects; Ataxia; Ataxia Telangiectasia Mutated Proteins; Background radiation; Biochemistry/ and Repair; BRCA1 protein; BRCA1 Protein - metabolism; Breast cancer; Cell Biology/Cell Signaling; Cell Biology/Nuclear Structure and Function; Cell cycle; Cell Cycle - drug effects; Cell Cycle - radiation effects; Cell Cycle Proteins - metabolism; Cell Line, Tumor; Cells (Biology); Cells, Cultured; Chromatin; Chromatin - metabolism; Critical components; Deoxyribonucleic acid; Dismantling; DNA; DNA adducts; DNA Damage; DNA repair; DNA-Activated Protein Kinase - metabolism; DNA-Binding Proteins - metabolism; Down-Regulation - drug effects; Down-Regulation - radiation effects; Drug dosages; Genes; Genotoxicity; HCT116 Cells; HEK293 Cells; HeLa Cells; Homologous recombination; Homology; Humans; Immunoblotting; Inhibition; Ionizing radiation; Kinases; Localization; Male; Medicine; Methyl Methanesulfonate - pharmacology; Molecular Biology/DNA Repair; Mutagens; Mutation; Ovarian cancer; Phosphatidylinositol 3-Kinases - metabolism; Phosphorylation; Phosphotransferases; Proteasomes; Protein-Serine-Threonine Kinases - metabolism; Proteins; Proteolysis; Rad51 Recombinase - metabolism; Rad52 protein; Radiation; Radiation damage; Radiation effects; Recruitment; Signal transduction; Signaling; Stress (Physiology); Studies; Transcription factors; Tumor suppressor genes; Tumor Suppressor Proteins - metabolism; Ubiquitin-Protein Ligases - metabolism; Ultraviolet Rays; Womens health; San Francisco California; California; United States > US; Massachusetts

The function of BRCA1 in response to ionizing radiation, which directly generates DNA double strand breaks, has been extensively characterized. However previous investigations have produced conflicting data on mutagens that initially induce other classes of DNA adducts. Because of the fundamental an...