Massive-scale RNA-Seq analysis of non ribosomal transcriptome in human trisomy 21

Hybridization- and tag-based technologies have been successfully used in Down syndrome to identify genes involved in various aspects of the pathogenesis. However, these technologies suffer from several limits and drawbacks and, to date, information about rare, even though relevant, RNA species such...

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Published inPloS one Vol. 6; no. 4; p. e18493
Main Authors Costa, Valerio, Angelini, Claudia, D'Apice, Luciana, Mutarelli, Margherita, Casamassimi, Amelia, Sommese, Linda, Gallo, Maria Assunta, Aprile, Marianna, Esposito, Roberta, Leone, Luigi, Donizetti, Aldo, Crispi, Stefania, Rienzo, Monica, Sarubbi, Berardo, Calabrò, Raffaele, Picardi, Marco, Salvatore, Paola, Infante, Teresa, De Berardinis, Piergiuseppe, Napoli, Claudio, Ciccodicola, Alfredo
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 20.04.2011
Public Library of Science (PLoS)
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Abstract Hybridization- and tag-based technologies have been successfully used in Down syndrome to identify genes involved in various aspects of the pathogenesis. However, these technologies suffer from several limits and drawbacks and, to date, information about rare, even though relevant, RNA species such as long and small non-coding RNAs, is completely missing. Indeed, none of published works has still described the whole transcriptional landscape of Down syndrome. Although the recent advances in high-throughput RNA sequencing have revealed the complexity of transcriptomes, most of them rely on polyA enrichment protocols, able to detect only a small fraction of total RNA content. On the opposite end, massive-scale RNA sequencing on rRNA-depleted samples allows the survey of the complete set of coding and non-coding RNA species, now emerging as novel contributors to pathogenic mechanisms. Hence, in this work we analysed for the first time the complete transcriptome of human trisomic endothelial progenitor cells to an unprecedented level of resolution and sensitivity by RNA-sequencing. Our analysis allowed us to detect differential expression of even low expressed genes crucial for the pathogenesis, to disclose novel regions of active transcription outside yet annotated loci, and to investigate a plethora of non-polyadenylated long as well as short non coding RNAs. Novel splice isoforms for a large subset of crucial genes, and novel extended untranslated regions for known genes--possibly novel miRNA targets or regulatory sites for gene transcription--were also identified in this study. Coupling the rRNA depletion of samples, followed by high-throughput RNA-sequencing, to the easy availability of these cells renders this approach very feasible for transcriptome studies, offering the possibility of investigating in-depth blood-related pathological features of Down syndrome, as well as other genetic disorders.
AbstractList Hybridization- and tag-based technologies have been successfully used in Down syndrome to identify genes involved in various aspects of the pathogenesis. However, these technologies suffer from several limits and drawbacks and, to date, information about rare, even though relevant, RNA species such as long and small non-coding RNAs, is completely missing. Indeed, none of published works has still described the whole transcriptional landscape of Down syndrome. Although the recent advances in high-throughput RNA sequencing have revealed the complexity of transcriptomes, most of them rely on polyA enrichment protocols, able to detect only a small fraction of total RNA content. On the opposite end, massive-scale RNA sequencing on rRNA-depleted samples allows the survey of the complete set of coding and non-coding RNA species, now emerging as novel contributors to pathogenic mechanisms. Hence, in this work we analysed for the first time the complete transcriptome of human trisomic endothelial progenitor cells to an unprecedented level of resolution and sensitivity by RNA-sequencing. Our analysis allowed us to detect differential expression of even low expressed genes crucial for the pathogenesis, to disclose novel regions of active transcription outside yet annotated loci, and to investigate a plethora of non-polyadenylated long as well as short non coding RNAs. Novel splice isoforms for a large subset of crucial genes, and novel extended untranslated regions for known genes--possibly novel miRNA targets or regulatory sites for gene transcription--were also identified in this study. Coupling the rRNA depletion of samples, followed by high-throughput RNA-sequencing, to the easy availability of these cells renders this approach very feasible for transcriptome studies, offering the possibility of investigating in-depth blood-related pathological features of Down syndrome, as well as other genetic disorders.
Hybridization- and tag-based technologies have been successfully used in Down syndrome to identify genes involved in various aspects of the pathogenesis. However, these technologies suffer from several limits and drawbacks and, to date, information about rare, even though relevant, RNA species such as long and small non-coding RNAs, is completely missing. Indeed, none of published works has still described the whole transcriptional landscape of Down syndrome. Although the recent advances in high-throughput RNA sequencing have revealed the complexity of transcriptomes, most of them rely on polyA enrichment protocols, able to detect only a small fraction of total RNA content. On the opposite end, massive-scale RNA sequencing on rRNA-depleted samples allows the survey of the complete set of coding and non-coding RNA species, now emerging as novel contributors to pathogenic mechanisms. Hence, in this work we analysed for the first time the complete transcriptome of human trisomic endothelial progenitor cells to an unprecedented level of resolution and sensitivity by RNA-sequencing. Our analysis allowed us to detect differential expression of even low expressed genes crucial for the pathogenesis, to disclose novel regions of active transcription outside yet annotated loci , and to investigate a plethora of non-polyadenilated long as well as short non coding RNAs. Novel splice isoforms for a large subset of crucial genes, and novel extended untranslated regions for known genes—possibly novel miRNA targets or regulatory sites for gene transcription—were also identified in this study. Coupling the rRNA depletion of samples, followed by high-throughput RNA-sequencing, to the easy availability of these cells renders this approach very feasible for transcriptome studies, offering the possibility of investigating in-depth blood-related pathological features of Down syndrome, as well as other genetic disorders.
Hybridization- and tag-based technologies have been successfully used in Down syndrome to identify genes involved in various aspects of the pathogenesis. However, these technologies suffer from several limits and drawbacks and, to date, information about rare, even though relevant, RNA species such as long and small non-coding RNAs, is completely missing. Indeed, none of published works has still described the whole transcriptional landscape of Down syndrome. Although the recent advances in high-throughput RNA sequencing have revealed the complexity of transcriptomes, most of them rely on polyA enrichment protocols, able to detect only a small fraction of total RNA content. On the opposite end, massive-scale RNA sequencing on rRNA-depleted samples allows the survey of the complete set of coding and non-coding RNA species, now emerging as novel contributors to pathogenic mechanisms. Hence, in this work we analysed for the first time the complete transcriptome of human trisomic endothelial progenitor cells to an unprecedented level of resolution and sensitivity by RNA-sequencing. Our analysis allowed us to detect differential expression of even low expressed genes crucial for the pathogenesis, to disclose novel regions of active transcription outside yet annotated loci, and to investigate a plethora of non-polyadenilated long as well as short non coding RNAs. Novel splice isoforms for a large subset of crucial genes, and novel extended untranslated regions for known genes-possibly novel miRNA targets or regulatory sites for gene transcription-were also identified in this study. Coupling the rRNA depletion of samples, followed by high-throughput RNA-sequencing, to the easy availability of these cells renders this approach very feasible for transcriptome studies, offering the possibility of investigating in-depth blood-related pathological features of Down syndrome, as well as other genetic disorders.
Audience Academic
Author Leone, Luigi
Donizetti, Aldo
Infante, Teresa
Gallo, Maria Assunta
Calabrò, Raffaele
Sommese, Linda
Costa, Valerio
Angelini, Claudia
Aprile, Marianna
Crispi, Stefania
Sarubbi, Berardo
Casamassimi, Amelia
Napoli, Claudio
Mutarelli, Margherita
Ciccodicola, Alfredo
Salvatore, Paola
Rienzo, Monica
De Berardinis, Piergiuseppe
D'Apice, Luciana
Picardi, Marco
Esposito, Roberta
AuthorAffiliation 3 Institute of Protein Biochemistry, CNR, Naples, Italy
4 Telethon Institute of Genetics and Medicine (TIGEM), Naples, Italy
1 Institute of Genetics and Biophysics “A. Buzzati-Traverso”, CNR, Naples, Italy
2 Istituto per le Applicazioni del Calcolo, Mauro Picone, CNR, Naples, Italy
Fondazione Telethon, Italy
10 Department of Cellular and Molecular Biology and Pathology “L. Califano”, University of Naples “Federico II” and Ceinge Biotecnologie Avanzate s.c.a.r.l., Naples, Italy
7 Centro Diagnostico San Ciro, Portici, Italy
6 Section of Microbiology, Department of Experimental Medicine, 1st School of Medicine, Second University of Naples, Naples, Italy
11 Fondazione-SDN (Institute of Diagnostic and Nuclear Development), IRCCS, Naples, Italy
8 Cardiology Department of Second University of Naples, “Monaldi Hospital”, Naples, Italy
5 Department of General Pathology and Excellence Research Centre on Cardiovascular Diseases, 1st School of Medicine, Second University of Naples, Naples, Italy
9 Department
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/21533138$$D View this record in MEDLINE/PubMed
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Cites_doi 10.1186/1471-2350-7-24
10.1038/nature08062
10.1093/bioinformatics/btp120
10.1093/hmg/11.26.3249
10.1111/j.1582-4934.2008.00639.x
10.1152/physiolgenomics.00125.2007
10.1371/journal.pbio.1000371
10.1186/1755-8794-3-40
10.1073/pnas.0813248106
10.1136/heart.89.6.597
10.1152/ajprenal.2000.279.2.F383
10.1016/j.bbrc.2008.03.120
10.1038/nrg2776
10.1371/journal.pone.0004108
10.1016/j.ygeno.2009.08.010
10.1126/science.1158441
10.1038/76469
10.1186/gb-2010-11-10-r106
10.1038/nmeth768
10.1016/S0888-7543(03)00035-1
10.1038/nature05874
10.1093/bioinformatics/btp459
10.1038/nmeth.1226
10.1038/nmeth.1223
10.1016/j.jacc.2006.09.050
10.1371/journal.pone.0016266
10.1161/hc1102.106792
10.1186/1471-2105-7-276
10.1093/bioinformatics/btp612
10.1126/science.1162228
10.1073/pnas.0803602105
10.1101/gr.079558.108
10.1161/ATVBAHA.107.155960
10.1016/j.ygeno.2008.05.011
10.1126/science.1160342
10.1016/S1470-2045(00)00435-6
10.1093/nar/gkl842
10.1182/blood-2002-09-2797
10.1073/pnas.2136655100
10.1016/j.crvi.2006.06.004
10.2174/138161207782341303
10.1186/1471-2164-7-246
10.1038/nature07672
10.1006/meth.2001.1262
10.1093/nar/gkn875
10.1139/G07-100
10.1093/bioinformatics/btl048
10.1186/gb-2010-11-2-r14
10.1093/nar/gkn828
10.1093/jb/mvm060
10.1093/nar/gkp1019
10.1038/nsmb.1841
10.1038/nmeth.1315
10.1016/j.braindev.2010.05.001
10.1161/ATVBAHA.109.189407
10.2144/000112900
10.1182/blood-2006-08-043471
10.1161/hh1301.093953
10.1006/bbrc.2000.2272
10.1126/science.270.5235.484
10.1016/j.molmed.2009.02.001
10.1097/GIM.0b013e3181772111
10.1261/rna.1528909
10.1016/j.mcn.2007.06.009
10.1093/hmg/ddm322
10.1161/CIRCULATIONAHA.108.769646
10.1093/bioinformatics/btp616
10.3233/JAD-2007-12402
10.1126/science.1191244
10.1155/2010/853916
10.1038/nrg2683
10.1016/j.exphem.2006.10.013
10.1007/s00277-005-0045-5
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DocumentTitleAlternate rRNA-Depleted Transcriptome in Down Syndrome
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Conceived and designed the experiments: VC CA LD'A CN A. Ciccodicola. Performed the experiments: VC CA LD'A MM A. Casamassimi MR LS MAG MA RE LL AD SC. Analyzed the data: VC CA LD'A MM A. Casamassimi MAG MA RE BS RC PS TI PDB CN A. Ciccodicola. Contributed reagents/materials/analysis tools: CA MM LS MAG AD SC BS RC MP PS PDB CN A. Ciccodicola. Wrote the paper: VC CA LD'A MM A. Casamassimi SC CN A. Ciccodicola.
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References MD Robinson (ref81) 2010; 26
T Takumi (ref35) 2010; 32
NA Twine (ref75) 2011; 6
H van Bakel (ref73) 2010; 8
KJ Livak (ref78) 2001; 25
MN Bainbridge (ref57) 2006; 7
G Esposito (ref21) 2008; 17
C Napoli (ref8) 2002; 105
F Hsu (ref63) 2006; 22
M Guttman (ref68) 2009; 458
VE Velculescu (ref4) 1995; 270
C Brochier (ref16) 2008; 33
L Wang (ref79) 2010; 26
T Yamashita (ref10) 2000; 269
J Lindberg (ref32) 2010; 95
AL Duker (ref36) 2010; Jun 30
CA Sommer (ref14) 2008; 51
GP Diller (ref40) 2008; 117
S Brenner (ref5) 2000; 18
M Vasa (ref50) 2001; 89
E Shantsila (ref46) 2007; 49
MA El-Meanawy (ref9) 2000; 279
MD Young (ref71) 2010; 11
C Napoli (ref49) 2007; 13
G Krenning (ref43) 2009; 15
W Malagó Jr (ref15) 2005; 46
D Langenberger (ref69) 2010; 2010
A Zampetaki (ref45) 2008; 78
S Hashimoto (ref28) 2009; 4
KH Baek (ref65) 2009; 459
MJ Okoniewski (ref1) 2006; 7
A Jacquier (ref33) 2009; 10
DR FitzPatrick (ref24) 2002; 11
E Pauws (ref11) 2000; 85
N Cloonan (ref59) 2009; 25
M Sultan (ref29) 2008; 321
E Grünblatt (ref17) 2007; 12
H Gremmels (ref77) 2011; Feb 14
DV Gnatenko (ref20) 2003; 101
V Costa (ref12) 2010; 3
F Sabatier (ref48) 2009; 13
S Anders (ref80) 2010
CM Li (ref22) 2006; 7
RJ Taft (ref67) 2009; 15
DE Kuhn (ref34) 2008; 370
H Mi (ref70) 2010; 38
V Costa (ref26) 2010; 2010
F Tang (ref27) 2009; 6
TJ Hubbard (ref64) 2009; 37
(ref25) 2007; 447
RJ Taft (ref66) 2010; 17
U Nagalakshmi (ref52) 2008; 320
CP Ponting (ref72) 2010; Sep 20
L Abildgaard (ref38) 2006; 85
C Napoli (ref2) 2003; 89
A Mortazavi (ref55) 2008; 5
B Jablonska (ref42) 2006; 329
RM Kuhn (ref62) 2009; 37
Z Yang (ref19) 2009; 29
LJ Core (ref53) 2008; 322
H Keren (ref74) 2010; 11
KK Hirschi (ref44) 2008; 28
A Casamassimi (ref58) 2007; 141
C Trapnell (ref76) 2009; 25
JO Korbel (ref13) 2009; 106
DK Holmes (ref41) 2006; 34
KD Pruitt (ref60) 2007; 35
EA Bruford (ref61) 2008; 36
MC Yoder (ref47) 2007; 109
T Shiraki (ref6) 2003; 100
JC Marioni (ref31) 2008; 18
R Rosenkranz (ref56) 2008; 92
PT Xu (ref18) 2007; 36
N Cloonan (ref30) 2008; 5
M Mangone (ref51) 2010; 329
P Salvatore (ref7) 2008; 105
R Mao (ref23) 2003; 81
R Morin (ref54) 2008; 45
AE Lin (ref37) 2008; 10
M Harbers (ref3) 2005; 2
H Hasle (ref39) 2001; 2
References_xml – volume: 7
  start-page: 24
  year: 2006
  ident: ref22
  article-title: Cell type-specific over-expression of chromosome 21 genes in fibroblasts and fetal hearts with trisomy 21.
  publication-title: BMC Med Genet
  doi: 10.1186/1471-2350-7-24
  contributor:
    fullname: CM Li
– volume: 459
  start-page: 1126
  year: 2009
  ident: ref65
  article-title: Down's syndrome suppression of tumour growth and the role of the calcineurin inhibitor DSCR1.
  publication-title: Nature
  doi: 10.1038/nature08062
  contributor:
    fullname: KH Baek
– volume: 25
  start-page: 1105
  year: 2009
  ident: ref76
  article-title: TopHat: discovering splice junctions with RNA-Seq.
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btp120
  contributor:
    fullname: C Trapnell
– volume: 11
  start-page: 3249
  year: 2002
  ident: ref24
  article-title: Transcriptome analysis of human autosomal trisomy.
  publication-title: Hum Mol Genet
  doi: 10.1093/hmg/11.26.3249
  contributor:
    fullname: DR FitzPatrick
– volume: 2010
  start-page: 80
  year: 2010
  ident: ref69
  article-title: Identification and classification of small rnas in transcriptome sequence data.
  publication-title: Pac Symp Biocomput
  contributor:
    fullname: D Langenberger
– volume: Sep 20
  year: 2010
  ident: ref72
  article-title: Transcribed dark matter: meaning or myth?
  publication-title: Hum Mol Genet
  contributor:
    fullname: CP Ponting
– volume: 13
  start-page: 454
  year: 2009
  ident: ref48
  article-title: Circulating endothelial cells microparticles and progenitors: key players towards the definition of vascular competence.
  publication-title: J Cell Mol Med
  doi: 10.1111/j.1582-4934.2008.00639.x
  contributor:
    fullname: F Sabatier
– volume: 33
  start-page: 170
  year: 2008
  ident: ref16
  article-title: Quantitative gene expression profiling of mouse brain regions reveals differential transcripts conserved in human and affected in disease models.
  publication-title: Physiol Genomics
  doi: 10.1152/physiolgenomics.00125.2007
  contributor:
    fullname: C Brochier
– volume: 8
  start-page: e1000371
  year: 2010
  ident: ref73
  article-title: Most “dark matter” transcripts are associated with known genes.
  publication-title: PLoS Biol
  doi: 10.1371/journal.pbio.1000371
  contributor:
    fullname: H van Bakel
– volume: 78
  start-page: 413
  year: 2008
  ident: ref45
  article-title: Vascular Repair by Endothelial Progenitor Cells Cardiovasc Res
  publication-title: Vascular Repair by Endothelial Progenitor Cells Cardiovasc Res
  contributor:
    fullname: A Zampetaki
– volume: 3
  start-page: 40
  year: 2010
  ident: ref12
  article-title: Impairment of circulating endothelial progenitors in Down syndrome.
  publication-title: BMC Med Genomics
  doi: 10.1186/1755-8794-3-40
  contributor:
    fullname: V Costa
– volume: 106
  start-page: 12031
  year: 2009
  ident: ref13
  article-title: The genetic architecture of Down syndrome phenotypes revealed by high-resolution analysis of human segmental trisomies.
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.0813248106
  contributor:
    fullname: JO Korbel
– volume: 89
  start-page: 597
  year: 2003
  ident: ref2
  article-title: Microarray analysis: a novel research tool for cardiovascular scientists and physicians.
  publication-title: Heart
  doi: 10.1136/heart.89.6.597
  contributor:
    fullname: C Napoli
– volume: 279
  start-page: F383
  year: 2000
  ident: ref9
  article-title: Use of serial analysis of gene expression to generate kidney expression libraries.
  publication-title: Am J Physiol Renal Physiol
  doi: 10.1152/ajprenal.2000.279.2.F383
  contributor:
    fullname: MA El-Meanawy
– volume: 370
  start-page: 473
  year: 2008
  ident: ref34
  article-title: Human chromosome 21-derived miRNAs are overexpressed in down syndrome brains and hearts.
  publication-title: Biochem Biophys Res Commun
  doi: 10.1016/j.bbrc.2008.03.120
  contributor:
    fullname: DE Kuhn
– volume: Jun 30
  year: 2010
  ident: ref36
  article-title: Paternally inherited microdeletion at 15q11.2 confirms a significant role for the SNORD116 C/D box snoRNA cluster in Prader-Willi syndrome.
  publication-title: Eur J Hum Genet
  contributor:
    fullname: AL Duker
– volume: 11
  start-page: 345
  year: 2010
  ident: ref74
  article-title: Alternative splicing and evolution: diversification, exon definition and function.
  publication-title: Nat Rev Genet
  doi: 10.1038/nrg2776
  contributor:
    fullname: H Keren
– volume: 4
  start-page: e4108
  year: 2009
  ident: ref28
  article-title: High-resolution analysis of the 5 -end transcriptome using a next generation DNA sequencer.
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0004108
  contributor:
    fullname: S Hashimoto
– volume: 95
  start-page: 1
  year: 2010
  ident: ref32
  article-title: The plasticity of the mammalian transcriptome.
  publication-title: Genomics
  doi: 10.1016/j.ygeno.2009.08.010
  contributor:
    fullname: J Lindberg
– volume: 320
  start-page: 1344
  year: 2008
  ident: ref52
  article-title: The transcriptional landscape of the yeast genome defined by RNA sequencing.
  publication-title: Science
  doi: 10.1126/science.1158441
  contributor:
    fullname: U Nagalakshmi
– volume: 18
  start-page: 630
  year: 2000
  ident: ref5
  article-title: Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays.
  publication-title: Nat Biotechnol
  doi: 10.1038/76469
  contributor:
    fullname: S Brenner
– year: 2010
  ident: ref80
  article-title: Differential expression analysis for sequence count data.
  doi: 10.1186/gb-2010-11-10-r106
  contributor:
    fullname: S Anders
– volume: 2
  start-page: 495
  year: 2005
  ident: ref3
  article-title: Tag-based approaches for transcriptome research and genome annotation.
  publication-title: Nat Methods
  doi: 10.1038/nmeth768
  contributor:
    fullname: M Harbers
– volume: 81
  start-page: 457
  year: 2003
  ident: ref23
  article-title: Global up-regulation of chromosome 21 gene expression in the developing Down syndrome brain.
  publication-title: Genomics
  doi: 10.1016/S0888-7543(03)00035-1
  contributor:
    fullname: R Mao
– volume: 447
  start-page: 799
  year: 2007
  ident: ref25
  article-title: Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project.
  publication-title: Nature
  doi: 10.1038/nature05874
– volume: 25
  start-page: 2615
  year: 2009
  ident: ref59
  article-title: RNA-MATE: a recursive mapping strategy for high-throughput RNAsequencing data.
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btp459
  contributor:
    fullname: N Cloonan
– volume: 5
  start-page: 621
  year: 2008
  ident: ref55
  article-title: Mapping and quantifying mammalian transcriptomes by RNA-Seq.
  publication-title: Nat Methods
  doi: 10.1038/nmeth.1226
  contributor:
    fullname: A Mortazavi
– volume: 5
  start-page: 613
  year: 2008
  ident: ref30
  article-title: Stem cell transcriptome profiling via massive-scale mRNA sequencing.
  publication-title: Nat Methods
  doi: 10.1038/nmeth.1223
  contributor:
    fullname: N Cloonan
– volume: 49
  start-page: 741
  year: 2007
  ident: ref46
  article-title: Endothelial progenitor cells in cardiovascular disorders.
  publication-title: J Am Coll Cardiol
  doi: 10.1016/j.jacc.2006.09.050
  contributor:
    fullname: E Shantsila
– volume: 6
  start-page: e16266
  year: 2011
  ident: ref75
  article-title: Whole transcriptome sequencing reveals gene expression and splicing differences in brain regions affected by Alzheimer's disease.
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0016266
  contributor:
    fullname: NA Twine
– volume: 36
  start-page: D445
  issue: Database issue
  year: 2008
  ident: ref61
  article-title: The HGNC Database in 2008: a resource for the human genome.
  publication-title: Nucleic Acids Res
  contributor:
    fullname: EA Bruford
– volume: 105
  start-page: 1360
  year: 2002
  ident: ref8
  article-title: Maternal hypercholesterolemia during pregnancy promotes early atherogenesis in LDL receptor-deficient mice and alters aortic gene expression determined by microarray.
  publication-title: Circulation
  doi: 10.1161/hc1102.106792
  contributor:
    fullname: C Napoli
– volume: 7
  start-page: 276
  year: 2006
  ident: ref1
  article-title: Hybridization interactions between probesets in short oligo microarrays lead to spurious correlations.
  publication-title: BMC Bioinformatics
  doi: 10.1186/1471-2105-7-276
  contributor:
    fullname: MJ Okoniewski
– volume: 26
  start-page: 136
  year: 2010
  ident: ref79
  article-title: DEGseq: an R package for identifying differentially expressed genes from RNA-seq data.
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btp612
  contributor:
    fullname: L Wang
– volume: 322
  start-page: 1845
  year: 2008
  ident: ref53
  article-title: Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters.
  publication-title: Science
  doi: 10.1126/science.1162228
  contributor:
    fullname: LJ Core
– volume: 105
  start-page: 9427
  year: 2008
  ident: ref7
  article-title: Detrimental effects of Bartonella henselae are counteracted by L-arginine and nitric oxide in human endothelial progenitor cells.
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.0803602105
  contributor:
    fullname: P Salvatore
– volume: 18
  start-page: 1509
  year: 2008
  ident: ref31
  article-title: RNA-Seq: an assessment of technical reproducibility and comparison with gene expression arrays.
  publication-title: Genome Res
  doi: 10.1101/gr.079558.108
  contributor:
    fullname: JC Marioni
– volume: 28
  start-page: 1584
  year: 2008
  ident: ref44
  article-title: Assessing identity phenotype and fate of endothelial progenitor cells.
  publication-title: Arterioscler Thromb Vasc Biol
  doi: 10.1161/ATVBAHA.107.155960
  contributor:
    fullname: KK Hirschi
– volume: 92
  start-page: 187
  year: 2008
  ident: ref56
  article-title: Characterizing the mouse ES cell transcriptome with Illumina sequencing.
  publication-title: Genomics
  doi: 10.1016/j.ygeno.2008.05.011
  contributor:
    fullname: R Rosenkranz
– volume: 46
  start-page: 647
  year: 2005
  ident: ref15
  article-title: Gene expression profile of human Down syndrome leukocytes.
  publication-title: Croat Med J
  contributor:
    fullname: W Malagó Jr
– volume: 321
  start-page: 956
  year: 2008
  ident: ref29
  article-title: A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome.
  publication-title: Science
  doi: 10.1126/science.1160342
  contributor:
    fullname: M Sultan
– volume: 2
  start-page: 429
  year: 2001
  ident: ref39
  article-title: Pattern of malignant disorders in individuals with Down's syndrome.
  publication-title: Lancet Oncol
  doi: 10.1016/S1470-2045(00)00435-6
  contributor:
    fullname: H Hasle
– volume: 35
  start-page: D61
  issue: Database issue
  year: 2007
  ident: ref60
  article-title: NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes transcripts and proteins.
  publication-title: Nucleic Acids Res
  doi: 10.1093/nar/gkl842
  contributor:
    fullname: KD Pruitt
– volume: 101
  start-page: 2285
  year: 2003
  ident: ref20
  article-title: Transcript profiling of human platelets using microarray and serial analysis of gene expression.
  publication-title: Blood
  doi: 10.1182/blood-2002-09-2797
  contributor:
    fullname: DV Gnatenko
– volume: 100
  start-page: 15776
  year: 2003
  ident: ref6
  article-title: Cap analysis gene expression for high-throughput analysis of transcriptional starting point and identification of promoter usage.
  publication-title: Proc Natl Acad Sci U S A
  doi: 10.1073/pnas.2136655100
  contributor:
    fullname: T Shiraki
– volume: 329
  start-page: 726
  year: 2006
  ident: ref42
  article-title: The growth capacity of bone marrow CD34 positive cells in culture is drastically reduced in a murine model of Down syndrome.
  publication-title: C R Biol
  doi: 10.1016/j.crvi.2006.06.004
  contributor:
    fullname: B Jablonska
– volume: 13
  start-page: 3245
  year: 2007
  ident: ref49
  article-title: Therapeutic approaches in vascular repair induced by adult bone marrow cells and circulating progenitor endothelial cells.
  publication-title: Curr Pharm Des
  doi: 10.2174/138161207782341303
  contributor:
    fullname: C Napoli
– volume: 7
  start-page: 246
  year: 2006
  ident: ref57
  article-title: Analysis of the prostate cancer cell line LNCaP transcriptome using a sequencing-by-synthesis approach.
  publication-title: BMC Genomics
  doi: 10.1186/1471-2164-7-246
  contributor:
    fullname: MN Bainbridge
– volume: 458
  start-page: 223
  year: 2009
  ident: ref68
  article-title: Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals.
  publication-title: Nature
  doi: 10.1038/nature07672
  contributor:
    fullname: M Guttman
– volume: 25
  start-page: 402
  year: 2001
  ident: ref78
  article-title: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
  publication-title: Methods
  doi: 10.1006/meth.2001.1262
  contributor:
    fullname: KJ Livak
– volume: 37
  start-page: D755
  issue: Database issue
  year: 2009
  ident: ref62
  article-title: The UCSC Genome Browser Database: update 2009.
  publication-title: Nucleic Acids Res
  doi: 10.1093/nar/gkn875
  contributor:
    fullname: RM Kuhn
– volume: 51
  start-page: 19
  year: 2008
  ident: ref14
  article-title: Identification of dysregulated genes in lymphocytes from children with Down syndrome.
  publication-title: Genome
  doi: 10.1139/G07-100
  contributor:
    fullname: CA Sommer
– volume: 22
  start-page: 1036
  year: 2006
  ident: ref63
  article-title: The UCSC Known Genes.
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btl048
  contributor:
    fullname: F Hsu
– volume: 11
  start-page: R14
  year: 2010
  ident: ref71
  article-title: Gene ontology analysis for RNA-seq: accounting for selection bias.
  publication-title: Genome Biol
  doi: 10.1186/gb-2010-11-2-r14
  contributor:
    fullname: MD Young
– volume: 37
  start-page: D690
  issue: Database issue
  year: 2009
  ident: ref64
  article-title: Ensembl 2009.
  publication-title: Nucleic Acids Res
  doi: 10.1093/nar/gkn828
  contributor:
    fullname: TJ Hubbard
– volume: 141
  start-page: 503
  year: 2007
  ident: ref58
  article-title: Comparison between total endothelial progenitor cell isolation versus enriched CD133+ culture.
  publication-title: J Biochem
  doi: 10.1093/jb/mvm060
  contributor:
    fullname: A Casamassimi
– volume: 38
  start-page: D204
  issue: Database issue
  year: 2010
  ident: ref70
  article-title: PANTHER version 7: improved phylogenetic trees orthologs and collaboration with the Gene Ontology Consortium.
  publication-title: Nucleic Acids Res
  doi: 10.1093/nar/gkp1019
  contributor:
    fullname: H Mi
– volume: 85
  start-page: 1923
  year: 2000
  ident: ref11
  article-title: Serial analysis of gene expression as a tool to assess the human thyroid expression profile and to identify novel thyroidal genes.
  publication-title: J Clin Endocrinol Metab
  contributor:
    fullname: E Pauws
– volume: 17
  start-page: 1030
  year: 2010
  ident: ref66
  article-title: Nuclear-localized tiny RNAs are associated with transcription initiation and splice sites in metazoans.
  publication-title: Nat Struct Mol Biol
  doi: 10.1038/nsmb.1841
  contributor:
    fullname: RJ Taft
– volume: 6
  start-page: 377
  year: 2009
  ident: ref27
  article-title: mRNA-Seq wholetranscriptome analysis of a single cell.
  publication-title: Nat Methods
  doi: 10.1038/nmeth.1315
  contributor:
    fullname: F Tang
– volume: 32
  start-page: 753
  year: 2010
  ident: ref35
  article-title: A humanoid mouse model of autism.
  publication-title: Brain Dev
  doi: 10.1016/j.braindev.2010.05.001
  contributor:
    fullname: T Takumi
– volume: 29
  start-page: 1213
  year: 2009
  ident: ref19
  article-title: Cardiovascular inflammation and lesion cell apoptosis: a novel connection via the interferon-inducible immunoproteasome.
  publication-title: Arterioscler Thromb Vasc Biol
  doi: 10.1161/ATVBAHA.109.189407
  contributor:
    fullname: Z Yang
– volume: 45
  start-page: 81
  year: 2008
  ident: ref54
  article-title: Profiling the HeLa S3 transcriptome using randomly primed cDNA and massively parallel short-read sequencing.
  publication-title: BioTechniques
  doi: 10.2144/000112900
  contributor:
    fullname: R Morin
– volume: 109
  start-page: 1801
  year: 2007
  ident: ref47
  article-title: Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals.
  publication-title: Blood
  doi: 10.1182/blood-2006-08-043471
  contributor:
    fullname: MC Yoder
– volume: 89
  start-page: E1
  year: 2001
  ident: ref50
  article-title: Number and migratory activity of circulating andothelial progenitor cells inversely correlate with risk factors for coronary artery disease.
  publication-title: Circ Res
  doi: 10.1161/hh1301.093953
  contributor:
    fullname: M Vasa
– volume: 269
  start-page: 110
  year: 2000
  ident: ref10
  article-title: Comprehensive gene expression profile of a normal human liver.
  publication-title: Biochem Biophys Res Commun
  doi: 10.1006/bbrc.2000.2272
  contributor:
    fullname: T Yamashita
– volume: 270
  start-page: 484
  year: 1995
  ident: ref4
  article-title: Serial analysis of gene expression.
  publication-title: Science
  doi: 10.1126/science.270.5235.484
  contributor:
    fullname: VE Velculescu
– volume: 15
  start-page: 180
  year: 2009
  ident: ref43
  article-title: Endothelial progenitor cell-based neovascularization: implications for therapy.
  publication-title: Trends Mol Med
  doi: 10.1016/j.molmed.2009.02.001
  contributor:
    fullname: G Krenning
– volume: 10
  start-page: 469
  year: 2008
  ident: ref37
  article-title: Adults with genetic syndromes and cardiovascular abnormalities: clinical history and management.
  publication-title: Genet Med
  doi: 10.1097/GIM.0b013e3181772111
  contributor:
    fullname: AE Lin
– volume: 15
  start-page: 1233
  year: 2009
  ident: ref67
  article-title: Small RNAs derived from snoRNAs.
  publication-title: RNA
  doi: 10.1261/rna.1528909
  contributor:
    fullname: RJ Taft
– volume: 36
  start-page: 313
  year: 2007
  ident: ref18
  article-title: A SAGE study of apolipoprotein E3/3 E3/4 and E4/4 allele-speciWc gene expression in hippocampus in Alzheimer disease.
  publication-title: Mol Cell Neurosci
  doi: 10.1016/j.mcn.2007.06.009
  contributor:
    fullname: PT Xu
– volume: 17
  start-page: 440
  year: 2008
  ident: ref21
  article-title: Genomic and functional profiling of human Down syndrome neural progenitors implicates S100B and aquaporin 4 in cell injury.
  publication-title: Hum Mol Genet
  doi: 10.1093/hmg/ddm322
  contributor:
    fullname: G Esposito
– volume: Feb 14
  year: 2011
  ident: ref77
  article-title: Transcriptome Analysis in Endothelial Progenitor Cell Biology.
  publication-title: Antioxid Redox Signal
  contributor:
    fullname: H Gremmels
– volume: 117
  start-page: 3020
  year: 2008
  ident: ref40
  article-title: Circulating endothelial progenitor cells in patients with Eisenmenger syndrome and idiopathic pulmonary arterial hypertension.
  publication-title: Circulation
  doi: 10.1161/CIRCULATIONAHA.108.769646
  contributor:
    fullname: GP Diller
– volume: 26
  start-page: 139
  year: 2010
  ident: ref81
  article-title: edgeR: a Bioconductor package for differential expression analysis of digital gene expression data.
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btp616
  contributor:
    fullname: MD Robinson
– volume: 12
  start-page: 291
  year: 2007
  ident: ref17
  article-title: Comparison analysis of gene expression patterns between sporadic Alzheimer's and Parkinson's disease.
  publication-title: J Alzheimers Dis
  doi: 10.3233/JAD-2007-12402
  contributor:
    fullname: E Grünblatt
– volume: 329
  start-page: 432
  year: 2010
  ident: ref51
  article-title: The landscape of C. elegans 3′UTRs.
  publication-title: Science
  doi: 10.1126/science.1191244
  contributor:
    fullname: M Mangone
– volume: 2010
  start-page: 853916
  year: 2010
  ident: ref26
  article-title: Uncovering the complexity of transcriptomes with RNA-Seq.
  publication-title: J Biomed Biotechnol
  doi: 10.1155/2010/853916
  contributor:
    fullname: V Costa
– volume: 10
  start-page: 833
  year: 2009
  ident: ref33
  article-title: The complex eukaryotic transcriptome: unexpected pervasive transcription and novel small RNAs.
  publication-title: Nat Rev Genetics
  doi: 10.1038/nrg2683
  contributor:
    fullname: A Jacquier
– volume: 34
  start-page: 1611
  year: 2006
  ident: ref41
  article-title: Hematopoietic progenitor cell deficiency in fetuses and children affected by Down's syndrome.
  publication-title: Exp Hematol
  doi: 10.1016/j.exphem.2006.10.013
  contributor:
    fullname: DK Holmes
– volume: 85
  start-page: 275
  year: 2006
  ident: ref38
  article-title: Optimal treatment intensity in children with Down syndrome and myeloid leukaemia: data from 56 children treated on NOPHO-AML protocols and a review of the literature.
  publication-title: Ann Hematol
  doi: 10.1007/s00277-005-0045-5
  contributor:
    fullname: L Abildgaard
SSID ssj0053866
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Snippet Hybridization- and tag-based technologies have been successfully used in Down syndrome to identify genes involved in various aspects of the pathogenesis....
Hybridization- and tag-based technologies have been successfully used in Down syndrome to identify genes involved in various aspects of the pathogenesis....
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StartPage e18493
SubjectTerms Alternative Splicing
Alzheimer's disease
Analysis
Biochemistry
Bioinformatics
Biology
Biophysics
Brain
Cardiology
Cells (biology)
Collaboration
Consortia
Dark matter
Down Syndrome
Down's syndrome
Endothelium
Feasibility studies
Gene Expression
Gene Expression Profiling
Gene sequencing
Genes
Genetic disorders
Genomes
Humans
Hybridization
Introns
Isoforms
Mammals
Medicine
miRNA
Non-coding RNA
Nucleic Acid Conformation
Ontology
Pathogenesis
Pathology
Phylogenetics
Ribonucleic acid
RNA
RNA - chemistry
RNA - genetics
RNA sequencing
rRNA
Sensitivity analysis
Stem cells
Transcription
Transcription (Genetics)
Trisomy
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Title Massive-scale RNA-Seq analysis of non ribosomal transcriptome in human trisomy 21
URI https://www.ncbi.nlm.nih.gov/pubmed/21533138
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http://dx.doi.org/10.1371/journal.pone.0018493
Volume 6
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