Comparison of Inducibility of Multidrug Resistance (MDR)1, Multidrug Resistance-Associated Protein (MRP)1, and MRP2 mRNAs by Prototypical Microsomal Enzyme Inducers in Primary Cultures of Human and Cynomolgus Monkey Hepatocytes

• Published in: Biological & Pharmaceutical Bulletin Vol. 31; no. 11; pp. 2068 - 2072
• Main Authors: Nishimura, Masuhiro, Koeda, Akiko, Morikawa, Hiroshi, Satoh, Tetsuo, Narimatsu, Shizuo, Naito, Shinsaku
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 01.11.2008; Pharmaceutical Society of Japan; Japan Science and Technology Agency
• Subjects: Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1 - biosynthesis; Cells, Cultured; Cryopreservation; cynomolgus monkey; Dexamethasone - pharmacology; Enzyme Induction; Female; hepatocyte; Hepatocytes - drug effects; Hepatocytes - enzymology; Hepatocytes - metabolism; Humans; induction; Macaca fascicularis; Microsomes, Liver - drug effects; Microsomes, Liver - enzymology; multidrug resistance 1; multidrug resistance-associated protein 1; multidrug resistance-associated protein 2; Multidrug Resistance-Associated Proteins - biosynthesis; Omeprazole - pharmacology; Reverse Transcriptase Polymerase Chain Reaction; Rifampin - pharmacology; RNA, Messenger - biosynthesis; Species Specificity

This study investigated the changes in the mRNA levels of the ATP binding cassette (ABC) transporters multidrug resistance 1 (MDR1), multidrug resistance-associated protein 1 (MRP1), and multidrug resistance-associated protein 2 (MRP2) following exposure to the prototypical microsomal enzyme inducer...