Monitoring of Antibiotic-Induced Alterations in the Human Intestinal Microflora and Detection of Probiotic Strains by Use of Terminal Restriction Fragment Length Polymorphism

• Published in: APPLIED AND ENVIRONMENTAL MICROBIOLOGY Vol. 71; no. 1; pp. 501 - 506
• Main Authors: Jernberg, Cecilia, Sullivan, Åsa, Edlund, Charlotta, Jansson, Janet K
• Format: Journal Article Publication
• Language: English
• Published: Washington, DC American Society for Microbiology 2005
• Subjects: analysis; Anti-Bacterial Agents; Anti-Bacterial Agents - pharmacology; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - growth & development; Bacteria - isolation & purification; Bacteriology; Biological and medical sciences; classification; clindamycin; Clindamycin - pharmacology; Colony Count, Microbial; community structure; DNA Fingerprinting; DNA Fingerprinting - methods; DNA, Bacterial; DNA, Bacterial - analysis; drug effects; Eubacterium; Eubacterium - genetics; Eubacterium - isolation & purification; feces; Fundamental and applied biological sciences. Psychology; genetics; growth & development; Humans; ingestion; Intestines; Intestines - drug effects; Intestines - microbiology; isolation & purification; Lactobacillus; Lactobacillus - genetics; Lactobacillus - isolation & purification; methods; microbial communities; Microbiology; monitoring; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; pharmacology; placebos; Polymorphism, Restriction Fragment Length; Probiotics; Public Health Microbiology; restriction fragment length polymorphism; volunteers; Human; Probiotic; Lactic acid bacteria; Antibiotic; Restriction fragment length polymorphism; Gut; Bacteria; Lactobacillaceae; Microflora; Detection; Lactobacillus

Terminal restriction fragment length polymorphism (T-RFLP) was investigated as a tool for monitoring the human intestinal microflora during antibiotic treatment and during ingestion of a probiotic product. Fecal samples from eight healthy volunteers were taken before, during, and after administratio...